Survey infection of Genetic diversity of E. coli strains isolated from beef cattle in sanandaj

Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)

Hiva karimi darhabi ¹

1 - Assistant Professor of Food Hygiene Department, College of Veterinary Science, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.

Received: 2022-08-05 Accepted : 2022-10-17 Published : 2022-11-22

Keywords: Escherichia coli, Phylogenetic groups, : beef cattle,

Abstract :

Escherichia coli often lead to foodborne pathogen and generally considered public health importance. Escherichia coli can be produce one or more Shiga toxins, which may produce diarrhea, hemorrhagic colitis and life-threatening hemolytic uremic syndromein humans and animals .The aim of the present work was to investigate the Escherichia coli groups A, B1, B2 and D of 80 samples from beef cattle in sanandaj . result shows that 34 samples(42.5%) E. coli isolated and identified based on standard procedures and PCR method with uidA gene .phylogenetic group of each strain was determined by using multiplex PCR method with chuA ,yjaA and segment of DNA TspE4C2. In this study, Among the strains isolated e.coli from beef cattle were allocated into phylogenetic group A (61.76%), B2 (5.8%), D(26.62%) and group B 1 (8.82%). Detection of E.coli isolation is very important and shows that food of animal origin can be of a reservoir that potentially could be transferred to humans the food chain.