List of Articles Ochratoxin

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  1 - Evaluating the relationship of fungal contamination and ochratoxin A content in nonalcoholic beers from different climatic regions
  Somayeh Alsadat Hosseini Khorami Reza Mahdavi Abolhasan Kazemi
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  2 - Evaluation of the effects of cold argon plasma jet on the clearance of Ochratoxin A produced by different isolates of Aspergillus nigri
  Hassanpour, S., Bayat, M., Chaichinosrati, A., Ghorannevis, M., Hashemi, S.J. .
  Mycotoxins are secondary metabolites of the fungi, and often arrange as cyclic hydrocarbons structurally. The aim of this study was an evaluation of the effects of cold argon plasma jet on the clearance of Ochratoxin A produced by isolates of Aspergillus nigri. food sou More

  Mycotoxins are secondary metabolites of the fungi, and often arrange as cyclic hydrocarbons structurally. The aim of this study was an evaluation of the effects of cold argon plasma jet on the clearance of Ochratoxin A produced by isolates of Aspergillus nigri. food sources including corn, wheat, oatmeal, rice and flour products were sampled of Iran. The fungal species were cultured for identification on CHAPK medium and also on (SB + YE) and (SB + ME) media for obtaining Ochratoxin A. The mean initial concentration of Ochratoxin A in ME + SB medium was 22.23 μg / kg and at 60 and 360 seconds reached to 38.36 and 4.82 μg / kg concentrations respectively, and in the YE + SB medium being at 38 / 34 μg / kg reached to 25.88 and 2.47 mg / kg concentrations, respectively. In comparison with Log / Lin with Lin / Log, the initial concentrations of Ochratoxin A in ME + SB medium was 39.224 μg/kg and at 60 and 360 seconds reached 22.26 and 8.414 μg / kg, respectively, and in the YE + SB medium from 31.50 to 22.68 and 8.95 μg / kg concentrations, respectively. Statistical analysis showed significant reduction of Ochratoxin changes produced by fungal isolates. This change was significant until the increase of 360 seconds with Plasma jet treatment in the environment (YE + SB), a decrease in the amount of Ochratoxin. Therefore, the jet plasma system can be used to eliminate fungal mycotoxins in order to increase the quality of food. Manuscript profile
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  3 - Designing Competitive Nanobiosensor for Ochratoxin based on FRET using quantum dot
  Mozaffari, M., Bayat, M., Mohsenifar, A., Hashemi, S.J. .
  In this research, using FRET (fluorescence resonance energy transfer) from Cd/Te quantum dot (anti Ochratoxin A antibody immobilized on the external surface of quantum dot or QDs) to (Ochratoxin A labeled with Rhodamine 123 bound to albumin), a sensitive competitive imm More

  In this research, using FRET (fluorescence resonance energy transfer) from Cd/Te quantum dot (anti Ochratoxin A antibody immobilized on the external surface of quantum dot or QDs) to (Ochratoxin A labeled with Rhodamine 123 bound to albumin), a sensitive competitive immunoassay was developed for measuring Ochratoxin A. The highly specific immune-reaction between anti-Ochratoxin A antibody on the QDs and labeled Ochratoxin A brings the Rho fluorophore and the QDs in close spatial proximity, and following photo-excitation of the QDs, causes FRET to occur between the Rho fluorophore (acting as the acceptor) and the QDs (acting as the donor). In the absence of free Ochratoxin A, the immune reaction between labeled Ochratoxin and the anti-Ochratoxin antibody on the QDs induces emission and causes FRET to occur. In the presence of free Ochratoxin A, it competes with the labeled Ochratoxin A-albumin complex for binding to the antibody-QDs conjugate in the Nanobiosensor, leading to reduction in Rhodamine emission after FRET. The reduction in the fluorescence intensity of the Rhodamine acceptor directly correlates with the concentration of free Ochratoxin A in the sample. This method has a detection limit of 220pg per ml. It has also been used to measure Ochratoxin A in human serum samples. A linear relationship is found between the increase in the fluorescence intensity of Rho 123 at580 nm and the concentration of OTA in spiked samples over the 100-800 pg⋅mL−1 concentration range. This highly sensitive homogeneous competitive detection scheme is simple, rapid and efficient. It does not require multiple separation steps and excessive washing. Manuscript profile
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  4 - Contamination rate of ochratoxin A in rice on Isfahan retail market
  ابراهیم Rahimi امیر Shakerian محسن Jafarian محمد Kajbafi
     Ochratoxin A (OTA) is a mycotoxin that possess a risk to human health due to its nephrotoxic, immunotoxic, mutagenic, teratogenic and carcinogenic consequences. This study was undertaken to determine the presence and levels of OTA in different types of rice More

     Ochratoxin A (OTA) is a mycotoxin that possess a risk to human health due to its nephrotoxic, immunotoxic, mutagenic, teratogenic and carcinogenic consequences. This study was undertaken to determine the presence and levels of OTA in different types of rice distributed in Isfahan.  For this, a total sample of 120 from 8 various types of rice was obtained from retail markets of Isfahan. Using ELISA method the samples were analyzed for the presence and contamination level of OTA. According to the kit manufacturer’s instruction, the recovery percentage of OTA in spiked rice samples at concentration of 5 ng/g was estimated at 84.9% and the detection limits for OTA was 0.625 ng/g. Analytical results showed a frequency of contamination of 20.8% of total rice samples. Levels of OTA in positive samples ranged from 1.07 to 10.83 ng/g and the average contamination level of samples was estimated at 3.5 ng/g. Among the samples, 3.3% were exceeded the permissible limits of 5 ng/g set by the European Regulations for OTA in cereals. According to the results, the daily intake of OTA via contaminated rice was estimated at 5.6 ng/kg bw/day.  Manuscript profile
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  5 - Survey of ochratoxin A in raisin produced in Hamadan province factories and relationship between contamination with defective seeds
  علی Heshmati علی اصغر Vahidinia مرضیه Jafari
     Ochratoxin A is one of the most common of raisin mycotoxins which is potential nephrotoxin in human and a teratogenic, mutagenic, carcinogenic and immunosuppressive toxin. The aim of this study was to determine the amount of ochratoxin in raisin produced in More

     Ochratoxin A is one of the most common of raisin mycotoxins which is potential nephrotoxin in human and a teratogenic, mutagenic, carcinogenic and immunosuppressive toxin. The aim of this study was to determine the amount of ochratoxin in raisin produced in Hamadan factories and its relationship with product quality (percentage of defective seeds). Sixty six raisin samples from 22 raisin producing plants in Hamadan province gathered in 2011-2012. Ochratoxin A from samples was extracted, cleaned up by immunoaffinity column and quantified with HPLC. Defective seed contents (in percentage) were determined by dividing unripe, damaged, moldy and sugary seed weight to sample weight (100 g). Ochratoxin average value and defective seed percentage was 1.72 μg/kg and 3.49%, respectively. Twenty three sampled had no ochratoxin; in 38 samples, Ochratoxin level was less than the national standard level (5 μg/kg); 5 samples had ochratoxin level more than 5 μg/kg. In all samples, defective seed percentage was less than the maximum allowable amount (10%). The apparent quality of raisin, defective seed percentage, was not significantly correlated with the amount of ochratoxin (P< 0.05).  Manuscript profile
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  6 - Determination of Ochratoxin A in the rainbow trout (Oncorhynchus mykiss) feed in Chaharmahal Va Bakhtiary province by ELISA assay
  فیروز Fadaeifard مهدی Raiisy حمید Koorangi ابراهیم Rahimi راحله Pirzadeh
     Ochratoxins are considered as the significant mycotoxins found in animal feeds. Amongst, Ochratoxin A has high pathological consequences on the humans and animals. The aim of present study was to determine the amount of Ochratoxin A in rainbow trout feed pr More

     Ochratoxins are considered as the significant mycotoxins found in animal feeds. Amongst, Ochratoxin A has high pathological consequences on the humans and animals. The aim of present study was to determine the amount of Ochratoxin A in rainbow trout feed produced in Chaharmahal Va Bakhtiary province. For this, four major producers of trout feed were chosen and four different sizes of feed together with one wheat flour sample were obtained from each factory. The samples were transferred to Food Analysis Lab of Shahre-Kord Islamic Azad University. The samples were obtained in three replicates and a total of 60 samples were analyzed for the presence of Ochratoxin A. The analysis was performed by ELISA assay. Results revealed that the quantity of Ochratoxin A in all feed samples were lower than determined contamination level established by Iranian National Standard and EU commission (5µg/kg). However, the contamination levels in all wheat flour samples were higher than defined standard. The amount of Ochratoxin A in samples obtained from various producers was not statistically significant (p<0.05). It was concluded that the overall quality of trout feed produced in Chaharmahal Va Bakhtiary province were satisfactory. Manuscript profile
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  7 - Biocontrol activity optimization of low-fermenting yeast isolates against Aspergillus niger to remove toxin from grape juice
  Arash Babaei Mohsen Duzbakhshan Hadis Tavafi
  Background & Objectives: Grapes infected with Aspergillus can produce ochratoxin A (OTA) in the processing of beverages such as wine or grape juice. This study aimed to evaluate the antifungal biological potential of two low-fermenting native yeast isolates (A01 G01 More

  Background & Objectives: Grapes infected with Aspergillus can produce ochratoxin A (OTA) in the processing of beverages such as wine or grape juice. This study aimed to evaluate the antifungal biological potential of two low-fermenting native yeast isolates (A01 G01) and three standards (Saccharomyces cerevisiae, Candida guilliermondii, Metschnikowia agaves) yeast isolates against Aspergillus niger and their ability to remove OTA in grape juice and its products, without any considerable alcohol production during the process. Material & Methods: Two native yeast isolates (A01 and G01) were obtained from Malayer apples and grapes, respectively, and inoculated on the PDA culture medium. Native isolates were identified by sequencing D1 and D2 and ITS 1 and ITS2 regions of the ribosomal DNA gene.Results: The results of DNA sequencing identified both native isolates as Saccharomyces. All strains showed a significant ability in inhibition of A. niger growth both on grape berries and in culture media. Meanwhile, yeast isolates produced a trace amount of alcohol.Conclusion: Biological control of A. niger and OTA-decontamination using yeast is proposed as an approach to meet the Islamic dietary laws regarding the absence of alcohol in halal beverages. Manuscript profile
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  8 - Occurrence of Zearalenone and Ochratoxin A in Cereals and Cereal Based Products
  Reza Kazemi Darsanaki Saeid Rahbar Ahmad Tajehmiri
  10.22034/jchr.2015.544120
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  9 - Survey of Mycotoxins in Wheat from Iran by HPLC Using Immunoaffinity Column Cleanup
  Javad Feizy Hamed Reza Beheshti Zeynab Eftekhari Mahnaz Zhiany
  10.22034/jchr.2018.544053
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  10 - Dietary Exposure of Filipinos to Ochratoxin A and Glyphosate from Commonly-Consumed Foods using Theoretical Maximum Daily Intake (TMDI) Approach
  Ruby Apilado Rose Elaine Guilaran Rhilen Audrey Teodoro Elyss Billedo
  10.22034/jchr.2023.1942487.1464
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