List of Articles Microfluidic

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  1 - Microfluidics and improving the cell culture channel: evaluation of spermatogonial stem cells using microfluidic chips
  S. Naeemi A.M. Kajbafzadeh, A. Eidi, R. Khanbabaee, H. Sadri-Ardekani
  The main purpose of the present study was to investigate the treatment of male infertility, because infertility treatment is important in the group of cancer patients treated with gonadotoxic drugs. The main approach of the mentioned study is to compare two different gr More

  The main purpose of the present study was to investigate the treatment of male infertility, because infertility treatment is important in the group of cancer patients treated with gonadotoxic drugs. The main approach of the mentioned study is to compare two different groups of spermatogonia stem cell culture methods and to evaluate the efficiency of differentiation and proliferation of these group of cells. Successful transplantation of spermatogonia stem cells (SSCs) in laboratory studies requires a suitable microenvironment for proliferation and differentiation of these cells. The natural extracellular matrix provides a good environment for stem cell culture. In the present study, themain purpose was to evaluate the ability of spermatogonial stem cells proliferation and differentiationvia the utilizing the microfluidic device (ex vivo). On the other hand, in the present study, we compared the obtained resultswith culture conditions in conventional culture plates (in vitro) to compare the SSCs proliferation and differentiation ability. In in-vitro culture method first spermatogonia stem cells from neonatal mice were isolated, then the resulted cells were seeded in culture plates on a scaffold consisting of hyaluronic acid, chitosan and decellularized testicular tissue, furthermore, in ex-vivo study the extracted spermatogonial stem cells were cultured in the microfluidic system without a scaffold. In ex vivo study, spermatogonial stem cells were extracted from neonatal male NMRI mice. The extracted cells were transferred to a microfluidic chip that was designed without an external pump, thereafter, the culture process was evaluated by IHC evaluation after one-month culture. In examined samples, cell attachment to the seminiferous tubules, DAPI staining and immunohistochemistry were evaluated. The results of immunohistochemical studies showed a significant increase in the expression of PLZF and TEKT1 markers in ex-vivo models. Finally, the results revealed that the ability of spermatogonia stem cells to induce spermatogenesis and production of haploid cells under testicular tissue culture in the microfluidic system is much more significant than conventional culture conditions in laboratory plates for these cells. Manuscript profile
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  2 - Optimization of Oleuropein Extraction from Olive Leaves using Artificial Neural Network
  Mahnaz Yasemi
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  3 - Studying the Shear and Discharge Rate of Proteins in Microfluidic Junctions, Under Electrokinetic EffectsStudying the Shear and Discharge Rate of Proteins in Microfluidic Junctions, Under Electrokinetic Effects
  Babak kamali Doust Azad Sasan Asiaei Borhan Beigzadeh
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  4 - Label-Free Perfect Separation of Michigan Cancer Foundation-7 Cells from White Blood Cells using Both Wall-Induced Lift and Dielectrophoresis Forces
  Alireza Alaghemand Ali Fattah
  10.30486/admt.2022.1957227.1347
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  5 - Design and Simulation of Novel Optofluidic ring resonator for Biomolecular Detection
  Keyvan Amini Saman Amini Hadi Veladi
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  6 - Evaluation of flow patterns maps of diclofenac sodium solvent extraction in microfluidic systems based on dimensionless numbers
  Mahvash Ansarimehr Ahmad Rahbar kelishami Hadi Shayesteh
  10.30495/jacr.2022.688383
  In the present study, the diclofenac (DF) solvent extraction from aqueous solution using tetra-n-butyl ammonium bromide (TBAB) in Y-type microfluidic junctions with liquid−liquid two-phase flow patterns was studied. Reynolds, Weber, and Capillary dimensionless num More

  In the present study, the diclofenac (DF) solvent extraction from aqueous solution using tetra-n-butyl ammonium bromide (TBAB) in Y-type microfluidic junctions with liquid−liquid two-phase flow patterns was studied. Reynolds, Weber, and Capillary dimensionless numbers have been used to investigate the competition between forces affecting the flow patterns. In low capillary and Weber numbers, a slug flow is formed, which indicates that at low velocities of two-phase tension is the force affecting the two-phase flow. With increasing the total flow rate from 1.2 mL/h to 2 mL/h, which is in the range of slug flow, the extraction efficiency decreased from 93% to 90.4%. With increasing the total flow rate from 4 mL/h to 12 mL/h, the slug flow became parallel, and the extraction efficiency decreased from 64.2% to 45.2%. By examining the mass transfer function of microchannels, it can be said that the higher the total flow rate (from 1.2 to 2 mL/h), the more the internal rotations increase, and as a result, the mass transfer coefficient increases from 0.131 1/s to 0.191 1/s. In parallel flow, with an increasing flow rate from 4 mL/h to 12 mL/h due to decreasing residence time and increasing the driving force of concentration, the mass transfer coefficient increases from 0.241 1/s to 0.283 1/s. Manuscript profile
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  7 - Study of α-Amylase recovery by aqueous two-phase system in micro dimensions
  Farshad Raji َAhmad Rahbar-Kelishami
  10.30495/jacr.2022.692119
  The microfluidic aqueous two-phase system is a new method and is a suitable platform for the separation and recovery of biomaterials in the field of biotechnology. The combination of aqueous two-phase systems with microfluidic systems provides features that are not poss More

  The microfluidic aqueous two-phase system is a new method and is a suitable platform for the separation and recovery of biomaterials in the field of biotechnology. The combination of aqueous two-phase systems with microfluidic systems provides features that are not possible or difficult to achieve in macro methods. According to studies, aqueous two-phase system can be considered as a low-cost, environmentally friendly, and effective method for the separation of biomolecules, but its combination with microfluidic systems adds to its attractiveness. In the studied system, due to its dimensions, it showed the potential ability to accelerate the design and separation of biological processes. Alpha-amylase recovery was investigated with an aqueous two-phase polyethylene glycol/sodium citrate system on a glass microfluidic device made by the Co2-laser method. Effective parameters were tested with different values of concentration (125-150 mg / L) and flow rate (0.8-2 mL / h). The response surface method (RSM) was used to optimally determine the operational parameters. Transfer of alpha-amylase from the salt phase to the polyethylene glycol phase was performed with a parallel flow pattern. The values obtained at the optimal point also had a small error compared to the predicted value of the experimental design equations. The use of the microfluidic system studied in this study due to the micro dimensions will increase the recovery rate compared to macro systems, as well as the reduction of the time of this process in the micro dimensions compared to macro dimensions was significant. Manuscript profile
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  8 - Continuous and controlled production of dexamethasone nanoparticles in a microfluidic system
  payam zahedi maryam tabatabai morteza fathi pour amin sohrabi
  The aim of this work is to produce continuously dexamethasone nanoparticles (DEX NPs) in a microfluidic (MF) system via nanoprecipitation method to control particle size, possessing their physical structure, and enhancing the efficiency of this hydrophobic drug in physi More

  The aim of this work is to produce continuously dexamethasone nanoparticles (DEX NPs) in a microfluidic (MF) system via nanoprecipitation method to control particle size, possessing their physical structure, and enhancing the efficiency of this hydrophobic drug in physiological environments. In order to fabricate a MF chip, a series of microchannels with dimensions 1 cm in length, 200 μm in width, and 50 μm in depth are embedded using ultraviolet soft lithography on a sheet based on polydimethylsiloxane (PDMS), and then the laminar fluid flow ability is investigated through it. The effective factors on the optimized production of the drug NPs are determined by the design of experiment. In this line, the optimum values for drug solution concentration, surfactant concentration, drug solution flow rate, and non-solvent flow rate are 15 mg/ml, 1 mg/ml, 4.5 ml/h, and 8 ml/h, respectively. By adjusting these values the average sizes of DEX NPs are obtained 590 ± 20 nm based on the model and 500 ± 20 nm according to the experiments. In the following, the results of dynamic light scattering (DLS) test show the narrow size distribution of DEX NPs fabricated using the MF chip. Also, scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and X-ray diffraction (XRD) assays reveal that application of the MF system does not affect the crystallinity of the drug NPs and does not alter their structure after the process. Finally, MF-assisted DEX NPs sample shows the drug solubility rate of about 8-fold compared to the commercial powder ones Manuscript profile