Nowadays demands for clean power source enhanced. Bio-fuel cell (BFC) can convert chemical energy to electrical energy. The enzyme-based biofuel cell (BFC) is a special fuel cell using enzyme as catalyst and can directly convert chemical energy to electrical energy. Bio More
Nowadays demands for clean power source enhanced. Bio-fuel cell (BFC) can convert chemical energy to electrical energy. The enzyme-based biofuel cell (BFC) is a special fuel cell using enzyme as catalyst and can directly convert chemical energy to electrical energy. Bio-fuel cells are energy conversion devices based on bio-electrocatalysis leveraging on enzymes or microorganisms. Enzymatic fuel cells are promising low cost, compact and flexible energy resources. The basis of enzymatic fuel cells is transfer of electron from enzyme to the electrode surface and vice versa.. The DET of laccase enzyme has been studied using Multi-walled carbon nanotubes (MWCNTs)/Nafion polymer. Laccase was entrapped by nafion polymer . As Nafion is a proton exchange polymer but not an electron conductive, the MWCNTs were also used to facilitate electron transfer of laccase. Cyclic voltammetric results showed a well-defined redox peaks at 170 µA in a solution containing 10 µM o-dianisidine as a substrate for MWCNTs/Nafion composite. The cyclic voltammetric results showed that laccase immobilization on MWCNTs/Nafion is efficient. Therefore, this method can be used to fabricate biocathode of biofuels cellor laccase based biosensors.
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Background & Objectives: Escherichia coli is an important indicator in the quality control of pharmaceutical and real samples. This study compares the detection of this bacterium by regular method and a biosensor based on Multi-Walled Carbon Nanotubes (MWCNTs) on glassy More
Background & Objectives: Escherichia coli is an important indicator in the quality control of pharmaceutical and real samples. This study compares the detection of this bacterium by regular method and a biosensor based on Multi-Walled Carbon Nanotubes (MWCNTs) on glassy carbon electrodes (GCE) in pharmaceutical and water as real sample.
Materials and Methods: In this experimental study, the conventional culture method (pour plate) and modified biosensor based on Multi-Walled Carbon Nanotubes on glassy carbon electrode with the arrangement of GC/MWCNTs/AuNPs/Ab/BSA were used for the detection of E. coli. Dilutions of E. coli between (1 ×101–1×108 CFU/ml) were used in pharmaceutical and water samples, prepared in 0.1 M PBS (pH 7.4), mixed with 0.5mM acetaminophen. The efficiency of the designed biosensor was investigated using SEM, Cyclic Voltammetry, and Square-Wave Voltammetry electrochemical techniques, as well as interfering bacteria.
Results: The results of E. coli detection using the conventional culture and designed biosensor were not statistically significant. The designed biosensor had a high sensitivity with accuracy in 3 minutes and LOD 3.02 CFU/ml for Escherichia coli.
Conclusion: Considering the time-consuming and influenced by environmental factors in the microbial monitoring of pharmaceuticals for E. coli detection in conventional methods and the risk of losing pharmaceutical products, the biosensor has good efficiency in detection with low cost and no need for enrichment in a small volume of samples.
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در دهه اخیر نانو سیالات پیشرفته ی جدیدی که از ذرات مختلف تشکیل شده اند مورد توجه محققان قرار گرفته اند. این گونه از نانوسیالات، که به نانوسیالات هیبریدی معروف هستند، عموما از ترکیب دوگونه مختلف نانوذرات ترکیب شده در سیال پایه به دست می آیند. در این مقاله، بررسی آ More
در دهه اخیر نانو سیالات پیشرفته ی جدیدی که از ذرات مختلف تشکیل شده اند مورد توجه محققان قرار گرفته اند. این گونه از نانوسیالات، که به نانوسیالات هیبریدی معروف هستند، عموما از ترکیب دوگونه مختلف نانوذرات ترکیب شده در سیال پایه به دست می آیند. در این مقاله، بررسی آزمایشگاهی اثر نانوذرات جامد هیبریدی نانولوله کربنی و اکسید منیزیم بر ضریب هدایت حرارتی اتیلن گلیکول ارائه شده است. آزمایشها در بازه دمایی 25 تا 50 درجه سانتی گراد برروی نمونه هایی با کسر حجمی 05/0٪، 1/0٪، 15/0٪، 2/0٪، 4/0٪ و 6/0٪ انجام شد. اندازه گیری ها نشان داد که با افزایش مقدار نانوذرات و افزایش دما، ضریب هدایت حرارتی تا 3/23٪ افزایش می یابد. در پایان یک رابطه تجربی جدید به منظور پیش بینی ضریب هدایت حرارتی ارائه شد و تحلیل حاشیه انحراف برای آن پیشنهادی انجام شد. نتایج این تحلیل ها نشان داد که حداکثر حاشیه انحراف 95/0٪ بود که بیانگر دقت قابل قبول رابطه پیشنهادی برای پیش بینی مقادیر ضریب هدایت حرارتی نانو سیال است
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