List of Articles Lipase

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  1 - The Effect of Enzyme-Based Improver on the Rheological Properties and Gluten Quality of Dough Obtained from Flour with Weak Gluten Index Bread
  M. Sarparast B. Ghiassi Tarzi
  Introduction: In recent years, the quality of Iranian bread has been drastically reduced. One of the reasons is the poor quality of gluten that has been produced from domestic wheat flour, which should be improved by special methods. Materials and Methods: In this resea More

  Introduction: In recent years, the quality of Iranian bread has been drastically reduced. One of the reasons is the poor quality of gluten that has been produced from domestic wheat flour, which should be improved by special methods. Materials and Methods: In this research, combination of three enzymes consisting of transglutaminase, glucose oxidase and phospholipase was used. In order to select the best combination of these three enzymes, Response Surface Methodology (RSM) optimal design was applied. The rheological properties of the dough were measured by Mixolab. The gluten index response was evaluated by centrifuging wet gluten for all the samples examined. Results: The results indicated that by increasing glucose oxidase and phospholipase enzymes simultaneously, the dough development time (C1) had a significant increase (p < 0.01), which indicates the synergistic effect of these two enzymes on C1 of the samples. Also, by increasing the level of transglutaminase enzyme, the dough development time, required torque for breaking the protein network (C2) and water absorption had a significant increase (p < 0.05( which indicates the positive effect of this enzyme on the improvement of rheological properties of the dough. Gluten index also had a significant increase with the increase of the(p < 0.01) and glucose oxidase concentrations (p < 0.05) that indicates the conversion of soft gluten with a high elasticity to tight gluten with desirable elasticity. Conclusion: It is therefore concluded that the optimum concentrations of the enzymes were 30 ppm for transglutaminase, 15 ppm for glucose oxidase, and 13.16 ppm for phospholipase. Manuscript profile
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  2 - Optimization of lipase Enzyme production in the native strain Halophile Haloarchaea Isolated from Iran southern soil
  hossein vahidinejad Ahmadali purbabaei Mohammad hossein razavian
  AbstractArchaea metabolites and enzymes widely used in various industries ,Because of the extremophiles nature and solidity And appropriate activities in the Hard industrial conditions. This study has been conducted According to the importance of lipolytic enzymes toler More

  AbstractArchaea metabolites and enzymes widely used in various industries ,Because of the extremophiles nature and solidity And appropriate activities in the Hard industrial conditions. This study has been conducted According to the importance of lipolytic enzymes tolerance saline and alkaline conditions In the food, pharmaceutical and health Industry .Therefore Survey Influence Type and percent of substrate, percent of salt, pH, temperature and incubation time on the growth of Arkia strain Also Lipase Enzyme production was The aim of this study. Optimal conditions for strain growth and lipase enzyme production with a Taguchi method was concluded Respectively in the pH8 and 8, substrate 0.5 % & 1.5% ,time 6 and 7 day , salt 19% & 25% , temperature 45 ºC & 35 ºC in the presence of tween oil and sweet Almond oil as the sole carbon source.According to above results ,can use this strain in the tanning industry and extraction of oil. Manuscript profile
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  3 - Optimization of lipase enzyme activity and variables of fermentation conditions containing grape juice substrate in Aspergillus niger culture medium
  Nima Zargaran Reza Habibipour Narges Ghobadi
  Objective: Lipase enzyme is one of the important enzymes in food, detergent and textile industries. Also, today, industries related to this enzyme such as processing of organic materials, synthesis of biosurfactants, beverages and cosmetic, pharmaceutical and food indus More

  Objective: Lipase enzyme is one of the important enzymes in food, detergent and textile industries. Also, today, industries related to this enzyme such as processing of organic materials, synthesis of biosurfactants, beverages and cosmetic, pharmaceutical and food industries have expanded significantly. In this regard, the aim of the current research is to optimize the activity of lipase enzyme and the variables of fermentation conditions containing grape juice substrate in Aspergillus niger culture medium. Materials and methods: In this research, for the first time, in order to achieve the maximum activity of the enzyme in the culture medium containing the carbon source of grape juice, the effective variables in the fermentation process of Aspergillus niger, i.e. temperature, acidity, concentration of grape juice and nitrogen source (yeast extract-peptone) with the help of Design Expert software was optimized. Findings: The acidity equal to 7.5, the amount of concentration of grape juice and the combination of yeast extract and peptone (with a ratio of two to one) were obtained with 1.5 and 0.75%, respectively. Lipase activity after optimizing the conditions of the culture medium was equal to 17.694 U/ml. Conclusion: Aspergillus niger records significant lipase activity in the culture medium with the factors of temperature, concentration of grape juice and yeast extract and peptone in its optimal amount and acidity equivalent to 7.5. Therefore, the carbon and nitrogen sources used in this research are suitable sources for use in the culture medium of this microorganism to produce lipase enzyme. Manuscript profile
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  4 - Antihyperlipidemic activity of a unani formulation in high fat diet-induced obese murine model
  Md Rafiul Haque Shahid Hussain Ansari
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  5 - Biodiesel Production from Castor Oil in the Presence of Lipase/calcium alginate Biocatalyst; Optimizing and Evaluation of Temperature, Catalyst Amount, and Methanol to Oil Ratio Effects
  Arash Davoodimehr Alireza Shakeri Mohammad Barati
  10.30495/jacr.2023.1978271.2089
  In this study, biodiesel was produced from castor oil in the presence of lipase/calcium alginate biocatalyst. Porcine pancreatic lipase was immobilized on calcium alginate and used in the esterification of castor oil to fatty acid methyl esters (FAMEs). The synthesized More

  In this study, biodiesel was produced from castor oil in the presence of lipase/calcium alginate biocatalyst. Porcine pancreatic lipase was immobilized on calcium alginate and used in the esterification of castor oil to fatty acid methyl esters (FAMEs). The synthesized calcium alginate was characterized using FTIR, FESEM and BET analysis. Also, after lipase immobilization, the protein content of the synthesized bio-catalyst, as well as the hydrolysis activity and the activity esterification activity were evaluated. In order to optimize the reaction of biodiesel production, the design of the experiment was carried out in SAS JMP Pro software. For this purpose, three factors of reaction temperature, amount of catalyst and ratio of methanol to oil were considered and data analysis was done using RSM. The results showed that the highest biodiesel yield (86.96%) is obtained at a temperature of 48.2oC, the amount of catalyst is 0.32 g, and the ratio of methanol to oil is 0.50. It was also found that high amounts of each of these factors have negative effect on the efficiency of biodiesel production, which is due to the inherent characteristics of the enzyme, including inactivation at higher temperatures and high methanol amounts, as well as coagulation and conformational changes in the high catalyst concentrations. Manuscript profile
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  6 - Molecular identification and optimization of lipase- producing strain Bacillus thuringiensis L26
  Farzaneh Karimian Mohammad Hassan Ghorbani Sayed Hossein Mirdamadian
  Background & Objectives: Lipases are important hydrolytic enzymes produced by various microorganisms such as bacteria. This enzyme is applied in various industries including food and pharmaceutical industries. This study aimed to isolate and identify lipase-producin More

  Background & Objectives: Lipases are important hydrolytic enzymes produced by various microorganisms such as bacteria. This enzyme is applied in various industries including food and pharmaceutical industries. This study aimed to isolate and identify lipase-producing bacteria from various sources to use in different industries.Materials & Methods: Sampling and screening of lipase- producing bacteria were carried out from wastewater and soils of Habib-abad refinery, wastewater and slugs of Golbahar oil factory, sheep’s tail fat, and sesame meal. To evaluate the enzymatic activity, bacterial isolates were cultured in lipid-based media, where the supernatant was used for the next assay. A quantitative assessment of enzymatic activity was performed using a spectrophotometer, in the presence of para-nitrophenol acetate at the temperature of 28°C. The identification of bacterial isolates was carried out by macroscopic, microscopic and molecular analysis.Results: Screening bacterial isolates and the results of enzymatic activity assays showed strain 31 as the superior one. Molecular analysis results identified this strain as Bacillus thuringiensis L26. The highest enzymatic activity and stability were obtained at the temperature of 48°C, pH value of 8.5, and in the presence of sodium chloride, calcium chloride, potassium chloride, magnesium chloride, zinc sulfate, and manganese chloride, respectively.Conclusion: Our results showed the stability of tested lipase enzymes under alkaline conditions and the presence of different cations. Therefore, further complementary tests are recommended o assess practical uses. Manuscript profile
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  7 - Effect of Exercise Type and Adenosine on the Concentration of Lipoprotein Lipase, Triglyceride and Very Low Lipoprotein in High Fat Diets Fed Rats
  Narjes Zarei Nematollah Nemati Tahereh Bagherpour
  10.22034/jchr.2019.665824
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  8 - Scrutiny Flora and identify the dominant fungal and bacterial rhizosphere microorganisms in the soil fertile productive chitinase and phospholipase enzymes in Iranshahr
  hadi hosseini iman hormozi najmeh shakib
  The aim of this study was to identify and isolate bacteria and fungi that produce phospholipase and chitinase enzymes in Iranshahr fertile logic soil to identify and produce biological fertilizers for farmers in the province using microorganisms compatible with the geog More

  The aim of this study was to identify and isolate bacteria and fungi that produce phospholipase and chitinase enzymes in Iranshahr fertile logic soil to identify and produce biological fertilizers for farmers in the province using microorganisms compatible with the geographical conditions of the region (secondary target). Sampling of soil from agricultural fields in fertile areas of Iranshahr city and soil sampling areas were diluted by 10+ 5. For Chitin, Shrimp substation, Sigma Aldrich USA and sterile egg yolk were used for phospholipase substrate. After merging the dedicated substrates bygeneral media culture, positive enzyme microstructures were identified and isolated by halo production. Enzymes were identified and confirmed by spectrophotometry testing and measurement of light absorption at 410 and 280 nm wavelengths. Samples were sent to South Korea's Macrogen Company for molecular identification. The predominant bacteria and fungi producing phospholipase were Bacillus subtilis and Mucor hiemalis, respectivelythe highest production of chitinase enzyme was observed in Pseudomonas putida and Mucor hiemalis microsatellites. Changes in pH showed that an increase in pH to alkalinity greatly reduced enzyme secretion. The highest levels of both enzymes were found in Mucor hiemalis Manuscript profile