List of Articles HeLa cells

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  1 - Evaluation of cytotoxic effect of Ruta graveolens L. Hydro alcoholic extract in human cervical cancer cells (HeLa)
  seyed milad mousavi jazayeri elahe ebrahimi Masoud parsania
  Abstract Introduction Cervical cancer is one of the most common cancers in women. In some cases, the lack of appropriate response to treatment with chemical drugs and the side effects of using synthetic drugs show necessary to achieve a natural drug that has minimal si More

  Abstract Introduction Cervical cancer is one of the most common cancers in women. In some cases, the lack of appropriate response to treatment with chemical drugs and the side effects of using synthetic drugs show necessary to achieve a natural drug that has minimal side effects. This study was evaluated effects of Hydroalcoholic extracts Ruta graveolens L. against of HeLa cell. Material and Methods HeLa cells were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum. Preparation of extract were done by maceration technique. This study were assessed the effects of different concentrations (250, 500, 750, 1000 and 1500 μg/ml) of Ruta graveolens L. on the proliferation of HeLa cells at 24, 48 and 72 hours after treatment of cells with extract and medium containing 1% serum by Trypan blue and MTT methods. Finding The cytotoxic concentration 50% (CC50) of the extract was determined on HeLa cell 1500 ug/ml. The results showed that concentrations of 1000 ug/ml of Ruta graveolens L. extract, after 72 hours showed a significant decrease in the proliferation of HeLa cells compared with control. Conclusion: The results of this study showed Ruta graveolens L. extract can inhibit the proliferation of HeLa cells. Keyword: Cytotoxic effect, Ruta graveolens L. extract, HeLa cells. Manuscript profile
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  2 - Effects of Cytotoxicity of Nanoparticles of Ag/Si_O_P /Gelatin on Uterus Cancer Cell Lines (Hela)
  سیده مبینا موسوی سروینه باغی عباسعلی دهپور جویباری صدف فرخنده
  Nowadays, cancer is the second cause of mortality in the world and among its variants; the most common cancer in women is the ovary cancer. One of the methods for cancer treatment is chemical therapy. Some nanoparticles can be considered as a sensitizer because they enh More

  Nowadays, cancer is the second cause of mortality in the world and among its variants; the most common cancer in women is the ovary cancer. One of the methods for cancer treatment is chemical therapy. Some nanoparticles can be considered as a sensitizer because they enhance the cytotoxicity due to oxidative stress and increase the free radicals, especially reactive oxygen species (ROS), within cells resulting in cell death. In this study, elevated synergistic effect of Ag/Si_O_P /gelatin nanoparticles as radiosensitizer was evaluated in presence of cobalt-60 gamma rays on human ovary cancer cell line. In this experimental and laboratories study ,after duplication and farming the Hela cell class, gelatin silver nanoparticles with10, 20, 40, 60, 80, 160 densities are effected by Hela cancer cells, and after 72 hours of using MTT, the cytotoxicity is evaluated and the data are analyzed by Elisa Reader and Anova. The Results shown that silver nanoparticles of (p≤5%) 20, 40, 80 density after 72hours, the cell growth is significantly decreased according to controlled group. Silver nanoparticles of different sizes and density of this study is shown that gelatinous nanoparticle have effective cell relation or cancer cell (Hela) and highly induced apoptosis in cells and caused the death of cancer cell and have the lower poisonous to natural body cells. Manuscript profile
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  3 - Assessing the levels of CREB after siRNA mediated knockdown in K562 cells
  زهرا دیلمی خیابانی مهدی بنان علی محمد اصغریان جلال قره سوران غلامرضا جوادی کیمیا کهریزی حسین نجم آبادی
  Background: CREB is an important downstream protein for many signaling pathways. By having efficient siRNAs against CREB, it may be possible to assess the role of molecules involved in signaling pathways in different cell types. In this research the efficiency of CREB k More

  Background: CREB is an important downstream protein for many signaling pathways. By having efficient siRNAs against CREB, it may be possible to assess the role of molecules involved in signaling pathways in different cell types. In this research the efficiency of CREB knockdown by 2 different siRNAs in HeLa cells has been studied. Materials and methods: HeLa cells were transfected by siRNAs using Lipofectamine 2000. RNA extraction and cDNA synthesis have been done. The efficiency of CREB knockdown has been assessed by quantitative relative real time PCR. According to our results only one of the siRNAs has a high level of inhibitory effect on CREB gene expression in HeLa cells. The expression of CREB by this siRNA was knocked-down by 64.4% in HeLa cells. Conclusion: By using efficient siRNA specifically for knocking down of CREB gene, we can apply this efficient siRNA in different cell types due to study of signaling pathway which CREB may be involved. Manuscript profile