• List of Articles Goldfish

      • Open Access Article

        1 - Sub-lethal effects of nano zinc oxide (ZnO NPs) on some hematological indices of goldfish (Carassius auratus)
        Seyed Aliakbar Hedayati Abdolreza Jahanbakhshi
        Background and Objective: Extensive development of nanotechnology and applications of nano-particles in different industries has caused devastating effects of nano-materials on organisms. This study aimed to evaluate the effects of nano zinc oxide on hematological facto More
        Background and Objective: Extensive development of nanotechnology and applications of nano-particles in different industries has caused devastating effects of nano-materials on organisms. This study aimed to evaluate the effects of nano zinc oxide on hematological factors of goldfish. Methods: In this paper we determine the range of concentrations of zinc oxide nano-particles to determine LC50 of gold fish at 24, 48, 72 and 96 hours respectively. Then, according to the LC50, separate experiments were designed to induce sub-lethal concentration of this substance on Hematological parameters were studied. Blood samples of goldfish fry were taken in the 7 days following exposure to lethal concentrations (50% concentration) of nano-ZnO nano-materials with fish that were not exposed. Findings: The results showed that the nano-particles could changes in blood parameters of gold fish by increasing red blood cell (RBC), hemoglobin, hematocrit, MCV in treatment was influenced by the nano-particles, was (P>0.05) and reduced white blood cell (WBC) in the treatment of fish exposed to lethal concentrations of nano-particles compared to the control group (P>0.05) but did not show significant change in MCH, MCHC indices. Discussion and Conclusions: In conclusion sub-lethal concentration of nano zinc oxide could affect hematological parameters of goldfish and this fish have more resistance to this kind of pollutant.  Manuscript profile
      • Open Access Article

        2 - Effects of Different Level Zinc Sulfate(ZnSO4) in Diet on the Hematocrit and Hematology Parameters in Gold fish(Carassius auratus)
        عبدالرضا Jahanbakhshi علی شعبانی شهاب قاضی حامد کلنگی میاندره
        v:* {behavior:url(#default#VML);} o:* {behavior:url(#default#VML);} w:* {behavior:url(#default#VML);} .shape {behavior:url(#default#VML);} >1024x768Normal0falsefalsefalseEN-USX-NONEAR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal More
        v:* {behavior:url(#default#VML);} o:* {behavior:url(#default#VML);} w:* {behavior:url(#default#VML);} .shape {behavior:url(#default#VML);} >1024x768Normal0falsefalsefalseEN-USX-NONEAR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} >Inroduction & ObjectiveMineral materials are the most important part of the fish nutrition subjects diet because they represent a very low quantity in the diet but they have a high effect on fish physiology and metabolism. The aim of this study was investigate the effect of different level of zinc sulfate (ZnSo4) on hematocrit and hematology for assess the effects of this mineral matter on goldfish health.Materials and Methods:Experimental diets prepared with four levels(four treatments and each treatment include three repetitions) 0, 25, 75 and 125 mg/kg. Healthy fish with average weight 3.3 ± 0.10 and average length 3.5 ± 0.2 was maintained in 400 liter tanks withtemperature 28 ± 1, pH 7.5 ± 0.5 and hardness 286 ± 1.2 and feed for 60 days and end the trial hematocrit and hematology parameter was measured.ResultsResult of analysis the hematological parameter of fish feed with different levels of zinc showed that white blood cells (WBC), mean corpuscular volume (MCV), mean cell hemoglobin (MCH) and mean cell hemoglobin concentration (MCHC) in our experimental treatments did not show a significant difference (p≥0.05) but in red blood cells (RBC), hematocrit (Ht) and hemoglobin (Hb) there was significant difference between treatments (p≤0.05). Red blood cell count, hematocrit and hemoglobin in zinc sulfate treatments (treatments 2, 3 and 4) had higher values and the highest value was in group 4 (150 mg kg zinc sulfate), which there was significant difference between2 and 3 treatments also hada significant difference with control group.Conclusion:The results shows that zinc in the diet stimulate the immune system and can have positive effects on the health and hematological factors of goldfish. Manuscript profile
      • Open Access Article

        3 - Histopathological study of gill in common carp (Cyprinus carpio) and goldfish (Carassius auratus) during exposure to lethal concentrations of nano-zinc oxide, nano copper oxide and nano titanium dioxide
        Aliakbar Hedayati Fatemeh Darabitabar hassan rezaei
        Apart from skin, gills are the first place of direct exposure of fish to toxins or pollutants therefore study of changes in gill structure is a suitable indicator of toxins or pollutants under stressful conditions. The aim of this study was to investigate pathological a More
        Apart from skin, gills are the first place of direct exposure of fish to toxins or pollutants therefore study of changes in gill structure is a suitable indicator of toxins or pollutants under stressful conditions. The aim of this study was to investigate pathological alterations of gill tissue in common carp during exposure to different nanoparticles. For this study, 210 fry common carp with a total length of 7.36±0.30 cm and goldfish with a total length of 6.50±0.43 cm in 6 treatments with 3 replicates in each treatment were placed in 60 liter aquariums in groups of 11 individuals. Gill tissue samples of common carp and goldfish were taken 7 days after exposure to 50% lethal concentration of nano-zinc oxide, nano copper oxide and titanium dioxide nanoparticles. Histological changes were evident in samples exposed to nano-zinc oxide, nano copper oxide and titanium dioxide nanoparticles while no detectable changes were observed in control gill. The symptoms observed in both fish consisted of gill hyperplasia, adhesion of secondary lamellae, increased levels of mucus and hyperemia. The most common complication observed was gill hyperplasia and an increase in the amount of mucus. The results of this study indicate that 50% lethal concentration of nanoparticles of titanium, copper and zinc can cause tissue damage and destruction. Also, sub-lethal toxicity of nano-zinc oxide is higher than nano copper oxide and nano titanium dioxide and causes much wider effects on gill tissue of common carp and goldfish.  Manuscript profile
      • Open Access Article

        4 - Molecular detection of Aeromonas hydrophila in the aquarium gold fish and cultured rainbow trout in Chaharmahal va Bakhtiary province
        firouz Fadaeifard
           Aeromonas hydrophilia   is the etiologic agent of motile aeromonas septicaemia, one of the most important bacterial diseases of fresh and marine water fishes. The aim of the present study was detection of A. hydrophilia in the aquarium goldfish an More
           Aeromonas hydrophilia   is the etiologic agent of motile aeromonas septicaemia, one of the most important bacterial diseases of fresh and marine water fishes. The aim of the present study was detection of A. hydrophilia in the aquarium goldfish and cultured rainbow trout in Chaharmahal va Bakhtiary province.  In this study 50 goldfish from aquarium fish shops and 60 rainbow trouts suspected of having the disease from 6 farms (10 fish in each farm) were randomly collected. The average weight in goldfish and rainbow trout samples were 3-5 g and 10-20 g, respectively. Sampling was performed from kidney and liver, and inoculated into blood agar and incubated at 22°C for 24 hours. Pure colonies which are grown on the mediums were tested by catalase, oxidase and gram staining, then those of gram-negative, catalase and oxidase positive were diagnosed, and cultured on Shotts-Rimler medium (as selective medium for A. hydrophila). These mediums were incubated at 22 °C for 24-48 h. The typical colonies were tested by using oligonucleotide primers of lip gene by PCR method. In light of molecular analysis of all specimens, 9 and 6 isolates from rainbow trout and gold fishes were identified as A. hydrophila respectively. Due to the detection of A. hydrophila in both cultured rainbow trout and aquarium goldfish, the bacteria can lead to septicemia with mortality if the health management principles are not observed in fish farming. Manuscript profile
      • Open Access Article

        5 - Effects of Gonadotropin Releasing Hormone (GnRHa) and Carp Pituitary Extract (CPE) on Spawning Success, Fecundity, Fertilization Success and Survival Rate in Goldfish Carassius auratus
        M. K. Ra'iatpishe B. Mojazi Amiri S. H. Mousavi Sabet Z. Moradkhani S. Mohebbi
        In this investigation effectiveness of a Gonadotropin hormone (GnRHa) and Carp pituitary extract (CPE) on spawning success, fecundity, fertilization success and survival rate in Goldfish Carassius auratus were studied.  The brood stocks of Goldfish were divided to More
        In this investigation effectiveness of a Gonadotropin hormone (GnRHa) and Carp pituitary extract (CPE) on spawning success, fecundity, fertilization success and survival rate in Goldfish Carassius auratus were studied.  The brood stocks of Goldfish were divided to 5 treatments were injected as follows: 10µg/kg BW GnRHa and 20µg/kg BW GnRHa and 3mg/kg BW CPE and 5mg/kg BW CPE. The females were injected in 2 times (with 12 hours distance) and the males were injected once (simultaneous with second injection of females). Finally, in the fishes that received hormones, all of them were ready for spawning, but non of control group’s fishes was ready for spawning. Total achievable eggs in hormonal treatment fishes on sequence in 10 µg/kgBW GnRHa, 20 µg/kgBW GnRHa, 3 mg/kgBW CPE & 5 mg/kgBW CPE   were ±211057.81, ±255741.51, ±196255.07 & 65±2429.01. Fertilizibility of the treatments on sequence were1399.2±47.55, 1772.7848±.47, 1179.5±49.19 & 1590.3±38.52. Hatchability of the treatments on sequence were 1206±32.96, 1491.4±36.83, 982.8±53.53 &1395.5±32.23 and the survival rates of larvae were 1092.9±33.41, 1336.17±41.3, 880.62±54.43 & 1230.74±32.89. Results showed that hormonal (CPE and GnRHa) induced propagation of Goldfish was successful. Relative fecundity, fertilizibility, hatchability and survival rates of larvae were not significantly different between treatments 1 and 3 and between treatments 2 and 4 (P<0.05) respectively. Dunnet test was showed significant differences among control and treatment groups.   Manuscript profile
      • Open Access Article

        6 - Dietary Effects of the Synbiotic Biomin Imbo on Survival and Growth Performance of the Oranda GoldfishCarassius auratus v
        سمانه بشکاردانا بابک مقدسی حامد منوچهری
        Dietary effects of the synbiotic “Biomin Imbo” on survival and growth performance of the Oranda Goldfish (Carassius auratus), was studied. Increasing of the fish biomass in shorter time and less prime cost was the main aim of the recent research. For 2 month More
        Dietary effects of the synbiotic “Biomin Imbo” on survival and growth performance of the Oranda Goldfish (Carassius auratus), was studied. Increasing of the fish biomass in shorter time and less prime cost was the main aim of the recent research. For 2 months, 150 young Oranda Goldfish were fed with the symbiotic “Biomin Imbo” (0, 0.5, 1, 1.5 and 2 g/kg in dry food), Standard length and body weight were being meseared twice a mounth (erery 2weeks) and growth indices including Body Weight Growth (BWG), Daily Growth Rate (DGR), Condition Factor (CF) and Special Growth Rate (SGR) were calculated at the end of the 2nd mounth. Results showed that the synbiotic “Biomin Imbo” increased the growth performance of the treatements and the best dosage of the symbiotic (BWG 53.76%, DGR 0.12%, CF 20.97 and SGR 0.76) in the Oranda Goldfish dry food was 2g/kg. Manuscript profile