Iran is rich in medicinally important plants and has an ancient herbal treatment methods. Ourwork is based on the study of some indigenous plants which show inhibitory effect of glucoseutilization, and are in use as hypoglycemic agent in traditional system of medicine. More
Iran is rich in medicinally important plants and has an ancient herbal treatment methods. Ourwork is based on the study of some indigenous plants which show inhibitory effect of glucoseutilization, and are in use as hypoglycemic agent in traditional system of medicine. Gymnemasylvestre, Momordica charantia and Eugenia jumbolana have been shown to possesshypoglycemic activity of varying degree. After preparation of glucose solutions and mentionedherbal aqueous extracts, quantative measurement of glucose was done by o-toluidine andglucose oxidase methods in various PH. The results in three different media revealed that,hypoglycemic activity is more prominent in neutral and basic media as compared to acidicmedium.
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Introduction: In recent years, the quality of Iranian bread has been drastically reduced. One of the reasons is the poor quality of gluten that has been produced from domestic wheat flour, which should be improved by special methods. Materials and Methods: In this resea More
Introduction: In recent years, the quality of Iranian bread has been drastically reduced. One of the reasons is the poor quality of gluten that has been produced from domestic wheat flour, which should be improved by special methods. Materials and Methods: In this research, combination of three enzymes consisting of transglutaminase, glucose oxidase and phospholipase was used. In order to select the best combination of these three enzymes, Response Surface Methodology (RSM) optimal design was applied. The rheological properties of the dough were measured by Mixolab. The gluten index response was evaluated by centrifuging wet gluten for all the samples examined. Results: The results indicated that by increasing glucose oxidase and phospholipase enzymes simultaneously, the dough development time (C1) had a significant increase (p < 0.01), which indicates the synergistic effect of these two enzymes on C1 of the samples. Also, by increasing the level of transglutaminase enzyme, the dough development time, required torque for breaking the protein network (C2) and water absorption had a significant increase (p < 0.05( which indicates the positive effect of this enzyme on the improvement of rheological properties of the dough. Gluten index also had a significant increase with the increase of the(p < 0.01) and glucose oxidase concentrations (p < 0.05) that indicates the conversion of soft gluten with a high elasticity to tight gluten with desirable elasticity. Conclusion: It is therefore concluded that the optimum concentrations of the enzymes were 30 ppm for transglutaminase, 15 ppm for glucose oxidase, and 13.16 ppm for phospholipase.
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Abstract The glucose-oxidase enzyme is widely used in industry. Glucose oxidase is an oxido-reductase enzyme that catalyses the oxidation of glucose to hydrogen peroxide and gluconic acid. In this study, the glucose oxidase enzyme, its structure and function, tragacanth More
Abstract The glucose-oxidase enzyme is widely used in industry. Glucose oxidase is an oxido-reductase enzyme that catalyses the oxidation of glucose to hydrogen peroxide and gluconic acid. In this study, the glucose oxidase enzyme, its structure and function, tragacanth polymer, different methods of enzyme immobilization and statistical modeling methods have been introduced. To investigate the enzyme activity of glucose oxidase using design of experiments (DOE) and response surface methodology (RSM), the results of 19 experiments, have been conducted in previous research, were used. For modeling and optimization of enzymatic activity, three variables of pH, temperature and immobilization time were selected and examined using the Design Expert software. Then, based on statistical modeling, a correlation was proposed that be able to determine the enzyme activity of the glucose oxidase. Using response surface methodology, maximum enzyme activity (1116.2 μmol/min) of the glucose oxidase was achieved at pH of 5.98, 37 ° C and 33.28 minutes. Finally we observed that our model results are accurately agreed with the experimental results.
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In the present research, nylon 6-silver (NS)/ Chitosan (CS)/Rosemary master-batch nanofibers was provided in order to be used in packaging food. Morphology and structure of the nanofibers was investigated using the Scanning Electron Microscope (SEM) and Fourier-transfor More
In the present research, nylon 6-silver (NS)/ Chitosan (CS)/Rosemary master-batch nanofibers was provided in order to be used in packaging food. Morphology and structure of the nanofibers was investigated using the Scanning Electron Microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR). The antibacterial activity of synthetic nanofibers was evaluated for E. Coli and S. Aureus bacteria. The antioxidant activity of oxidant glucose of glucose oxidase and Rosemary as well as the function of the provided membranes in packaging and maintaining strawberry was investigated. Morphology and diameter of synthetic nanofibers were determined with SEM images. According to the results of the carried out investigations on the deterrent effect of silver on the activity of glucose oxidase of 70/30 NS-CS sample was selected as the optimum for subsequent studies, and based on the results, the highest enzyme activity (79.5%) with 0.05 grams of glucose oxidase was obtained. The antibacterial test of the membranes showed the perfect antibacterial activity of such membranes against the negative and gram-positive and gram-negative bacteria. In addition, the FTIR spectroscopy approved the presence of glucose oxidase, silver, and Rosemary in synthetic membranes.
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The present investigation was aimed to develop an intelligent closed-loop insulin delivery system based on carboxymethyl cellulose-g-polyacrylamide )CMC-g-PAAm( hydro gel. To control the release of insulin in response to the concentration of glucose, glucose oxidase )GO More
The present investigation was aimed to develop an intelligent closed-loop insulin delivery system based on carboxymethyl cellulose-g-polyacrylamide )CMC-g-PAAm( hydro gel. To control the release of insulin in response to the concentration of glucose, glucose oxidase )GOx( was immobilized in the hydro gel. The hydro gels were prepared by polymerization of AAm in the presence of CMC via a free radical polymerization method. Fourier-transform infrared spectroscopy )FTIR(, thermo gravimetric analysis )TGA/DTG(, and scanning electron microscope )SEM( were used to characterize the structure of the insulin loaded hydro gels. The sensitivity of the intelligent drug carrier to glucose concentration was investigated by UV-Vis Spectroscopy at 280nm.Optimization of the hydro gels loading characteristics was studied by full factorial design. Finally, insulin release from the synthesized drug delivery system was investigated by various kinetic models
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Background & Objectives: Glucose oxidase has a wide range of application in medical, pharmaceutical, food, textile, and environmental industries. Heterologous expression of the enzyme has been investigated in yeast hosts due to several industrial-scale limitations i More
Background & Objectives: Glucose oxidase has a wide range of application in medical, pharmaceutical, food, textile, and environmental industries. Heterologous expression of the enzyme has been investigated in yeast hosts due to several industrial-scale limitations in enzyme production by Aspergillus niger. The present study aimed to investigate bioinformatics characteristics of recombinant glucose oxidase in Yarrowia lipolytica Po1g-GOX and to optimize the production conditions of the enzyme by Taguchi experimental design method. Materials & Methods: Protein sequence, primary, secondary and tertiary structures and glycosylation rate of recombinant glucose oxidase enzyme were studied via bioinformatics tools. Optimization of recombinant glucose oxidase for glucose, peptone, yeast extract and pH at four levels in YPD media with thiamine were defined to Qualitek-4 software. The level of enzyme production was measured and analyzed by the software.Results: Recombinant glucose oxidase had 605 amino acids, with 29% α-helix, 16% β sheet and 54% coil in its secondary structure showing similarity to that of native glucose oxidase of A. niger. Eight glycosylation sites have been located in this recombinant protein, and the tertiary structure had one domain showing high similarity to native glucose oxidase. The highest level of recombinant glucose oxidase production was obtained in medium containing 30 g/L glucose, 5 g/L yeast extract, 15 g/L peptone, and pH 7.Conclusion: Recombinant glucose oxidase produced in Yarrowia has a suitable potential to be used in pharmaceutical and food industries due to high similarity to native glucose oxidase.
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