List of Articles ESBL

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  1 - ESBL prevalence and molecular characterization of beta-lactamase gene blaTEM in urinary isolates of Klebsiella pneumoniae in Qom
  منصوره نرگسیان Mohsen Zargar Mahmoud Saffari
  Klebsiella is opportunistic pathogen and one of the most common causes of nosocomial infections this pathogen cause a variety of diseases including urinary tract infections.Today, the prevalence of ESBL-producing pathogens (ESBLS) is important, so these infections are a More

  Klebsiella is opportunistic pathogen and one of the most common causes of nosocomial infections this pathogen cause a variety of diseases including urinary tract infections.Today, the prevalence of ESBL-producing pathogens (ESBLS) is important, so these infections are associated with prevalence of diseases and increase healthcare costs associated. The aim of this study was to determine the prevalence of genes blaTEM isolates of Klebsiella pneumoniae isolated from urinary tract infections in Qom.After identification of isolates using culture and biochemical methods, 140 isolates of Klebsiella pneumoniae was identified. The sensitivity of the isolates to different antibiotics was performed by disk diffusion method based on the standard (CLSI2013). The phenotypic confirmatory test ESBL strains have done followed by PCR method to detection of blaTEM gene. Out of 300 samples, 140 isolate identified as Klebsiella pneumoniae that 52 (%37.14) isolates were ESBL. The most antibiotic resistance was related to ceftazidime (%50.72). Out of 52 ESBL positive strains, 32 (61.53%) strains were shown to have blaTEM gene.Considering the high prevalence of ESBL-producing isolates in hospitals, early detection and follow-up to prevent the spread of resistant isolates it all the more essential. It must also change in the pattern of antibiotics, hospital infection control measures are highly recommended. Manuscript profile
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  2 - Evaluation of the prevalence of Escherichia coli resistant euroopathogen in patients suffering from urinary tract infection in Qom hospitals
  zeynab adnan
  Review : Background: Urinary tract infection is one of the most commonly reported nosocomial infections caused by Escherichia coli bacteria. This bacterium is located in the urinary tract epithelium and can cause inflammation of the urethra and inflammation of the urete More

  Review : Background: Urinary tract infection is one of the most commonly reported nosocomial infections caused by Escherichia coli bacteria. This bacterium is located in the urinary tract epithelium and can cause inflammation of the urethra and inflammation of the ureter, bladder and kidney. This bacterium has the ability to form a biofilm. It is resistant to the third generation of antibiotics, which has become a complex problem in the medical community. Materials and Methods: 100 urine samples from patients with UTI were collected at Qom hospitals level and their antibiotic resistance was evaluated using Kirby-Bauer method. The hybrid disc method was used to determine the isolates of broad-spectrum beta-lactamases (ESBLs). Results: Out of 100 samples with common biochemical tests, 75 samples were identified as E.coli E.p. The results showed that Escherichia coli euroopatogen had the highest antibiotic resistance to gentamicin 93% and about 60% of the samples were ESBL. Conclusion: Considering the antibiotic resistance of isolated bacteria from the urinary system and the presence of strains resistant to antibiotics in broad-spectrum beta-lactamases, it is necessary to increase the level of information of the individuals in society as against the release of such bacteria Did something. Keyword: Escherichia coli europathogen, ESBL, Urinary tract infection Manuscript profile
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  3 - Antibiotic resistance to beta-lactams in bacteria: Beta- Lactamases
  Fatemeh Noorbakhsh
  10.30495/zisti.2023.1972119.1141
  Introduction: B- lactam antibiotics is one of the most important antibiotic used worldwide for bacterial infectiouse diseases. By producing beta-lactamase enzymes in bacteria the central ring in beta-lactam antibiotics is hydrolyzed, and antibiotic inactivate and develo More

  Introduction: B- lactam antibiotics is one of the most important antibiotic used worldwide for bacterial infectiouse diseases. By producing beta-lactamase enzymes in bacteria the central ring in beta-lactam antibiotics is hydrolyzed, and antibiotic inactivate and develop resistance.Aim: In this article, it is discussed.a brief review of various methods of inactivation of beta-lactam antibiotics by bacteria.Method: This review article has been written by studying the articles that have been published in scientific journals regarding the inactivation mechanisms of various beta-lactam antibiotics. Results and Conclusion: Beta-lactam antibiotics, by binding to the penicillin-binding protein present in the bacterial cell membrane, inhibit transpeptidases and break down the peptidoglycan, resulting in the destruction of the cell wall and the death of the bacteria. Beta-lactamase enzymes hydrolyze and inactivate beta-lactam antibiotics before they reach the penicillin-binding protein in the cytoplasmic membrane. So far, more than 140 different types of beta-lactamase have been identified, which are classified based on different criteria and effective on different classes of beta-lactam antibiotics. Manuscript profile
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  4 - Detection of bla TEM, bla OXA and bla SHV genes in Escherichia coli isolated from colibacillosis in poultry by multiplex-PCR
  zohreh mehranifar mitra salehi kiyoumars amini
  Abstract    Antimicrobial resistance exhibited by extended-spectrum b-lactamases (ESBLs) production by E. coli is considered to be a major threat for colibacillosis in poultry. The present study deals with the detection of bla TEM, bla OXA and bla SHV genes i More

  Abstract    Antimicrobial resistance exhibited by extended-spectrum b-lactamases (ESBLs) production by E. coli is considered to be a major threat for colibacillosis in poultry. The present study deals with the detection of bla TEM, bla OXA and bla SHV genes in E. coli isolated from colibacillosis by MPCR.A total of 60 strains of E. coli were recovered from the poultry in Kerman, Iran. Cellular DNA was extracted by CinnaPure-DNA (Cell culture, Tissues, Gram negative Bacteria and CSF) and MPCR was performed for the identification of the OXA, SHV and TEM genes.CDT test results showed that 45 strains (75%) were noted for ESBL production. A total of 19 and 13 strains (31.6% and 21.6%) were positive for blaOXA and bla aada, respectively and 7 isolates (11.6%) carried both OXA and aada genes. All isolates were negative for TEM and SHV genes.The results showed, although disk diffusion is a highly practical and cost effective method for identification of ESBLs, but the novel multiplex PCR assay designed in this study may be routinely used in veterinary diagnostic laboratory for identification of extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae. Manuscript profile
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  5 - Determining antibiotic resistance pattern in Enterobacter cloacae strains isolated from checken and sheep meat in Shahrekord city
  elahe barzam الهه تاج بخش Hassan Momtaz
  Usually, contaminated food is one of the main causes of human infections, and in this case, poultry meat and sheep are considered as the main causes. Enterobacter cloacae strains, having different virulence factors and multiple antibiotic resistance are mainly considere More

  Usually, contaminated food is one of the main causes of human infections, and in this case, poultry meat and sheep are considered as the main causes. Enterobacter cloacae strains, having different virulence factors and multiple antibiotic resistance are mainly considered as an opportunistic pathogen. In this research, the isolation of Enterobacter cloacae from chicken and sheep meat samples in Shahrekord in 2019 was done by microbial and molecular methods. Antibiotic resistance pattern was determined by disc diffusion method and microtitre plate method was used to check biofilm production. The ability to produce broad-spectrum β-lactamase enzymes was investigated through phenotypic and genotypic methods. Out of 384 examined samples, Enterobacter cloacae were identified in 25 samples (6.51%) which also confirmed in the presence of the hsp60 in molecular analysis. Among these, 18 samples were related to chicken meat (72%) and 7 samples (28%) were related to sheep meat. The highest antibiotic resistance to cotrimoxazole and cefotaxime was reported in 20 isolates (80%) and the lowest resistance to nitrofuranthein was reported in 15 isolates (23.8%). In microtiter method. 15 isolates (60%) showed strong biofilm reaction, 10 isolates (40%) showed moderate biofilm reaction. The present study indicates that ESBL-producing Enterobacter cloacae strains have a relatively high prevalence. The increase in the number of these strains is often caused by the irrational prescription of antibiotics, which requires the use of new antimicrobial agents,. Manuscript profile
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  6 - Investigation of ampC & esbl genes in Escherichia coli isolated from human and poultry
  Elham Farrokhnazar Pejvak Khaki Soheila Moradi Bidhendi
  Background & Objectives: Beta-lactam antibiotics are currently the most common treatment for bacterial infections. The production of beta-lactamase enzymes are the most important reason of bacterial resistance to these antibiotics. The aim of this study was to inves More

  Background & Objectives: Beta-lactam antibiotics are currently the most common treatment for bacterial infections. The production of beta-lactamase enzymes are the most important reason of bacterial resistance to these antibiotics. The aim of this study was to investigate the presence of ampC and esbls genes in E. coli isolated from human and poultry. Materials & Methods: In this cross-sectional study, 400 urine samples were collected from medical centers and also 200 swab poultry cloaca samples were collected from poultry farms located in Tehran province. Phenotypic identification of the beta-lactamase producing strains was performed using disk diffusion method. The presence of ampC and esbls genes in bacteria was studied using PCR approach. Results: A total of 120 (30%) human sample and 50 (25%) poultry samples were infected to E. coli. Phenotyping evaluation showed that 54 cases (45%) of the human samples carried esbls beta-lactamase gene while 2 cases (1.67%) carried ampC beta-lactamase gene. In poultry samples, 3 cases (21.4%) were confirmed for ESBLs enzymes and none of them carried ampC gene. Based on genotyping analysis 2 (1.67%) of the strains isolated from human samples carried ampC gene. Conclusion: Based on the results of this study, the ampC beta-lactamase gene was found in human samples, but more accurate studies are required for poultry. Due to high risk factor of the beta-lactamase producing organisms in nosocomial infections further studies is suggested to prevent their spread in community. Manuscript profile
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  7 - Frequency of cas genes in the CRISPR/Cas system in ESBL-producing Escherichia coli isolated from urinary tract infection
  Neda Merikhi Jamileh Nowroozi ali nazemi Mehrdad Hashemi Robab Rafiei Tabatabaei
  10.30495/jmw.2022.1934160.1987
  Background & Objectives: CRISPR  system (clustered regularly interspaced short palindromic repeats) and Cas portions is a part of the immune system in microorganisms. The cas genes could be involved in reducing antibiotic resistance in bacteria. The aim of the More

  Background & Objectives: CRISPR  system (clustered regularly interspaced short palindromic repeats) and Cas portions is a part of the immune system in microorganisms. The cas genes could be involved in reducing antibiotic resistance in bacteria. The aim of the study was to investigate the frequency of cas genes of the CRISPR/Cas system in Extended Spectrum        Beta-Lactamase (ESBL) producing Escherichia coli isolated from urine culture of patients with urinary tract infection. Materials & Methods: In this cross-sectional study, 437 positive urine culture samples were collected from Chalus hospitals. Escherichia coli strains were isolated based on standard biochemical tests and Enterobacteriaceae commercial diagnostic kit, as well as antibiotic sensitivity using disc diffusion method (Kerby Baer). Combined disk test was conducted for isolates that were resistant to at least one of the third-generation cephalosporins in the foregoing antibiotic susceptibility test. Molecular identification of cas1,cas2,cas3,cas7 and cas5 genes was performed using the PCR method. Results: Out of 437 urin culture samples, 106 samples (24.3%) had E.coli infection. The highest antibiotic resistance was associated with ampicillin (99%). Among the resistant isolates, thirty isolates (88.3%) were ESBL producing. cas1 gene had the highest frequency (96.2%) and other cas genes had almost the same frequency. Conclusion: The results of the present study showed that a significant percentage of E. coli isolates had ESBL phenotype, which may be due to the presence of broad-spectrum beta-lactamase genes in these samples. Besides,  it was shown that there is no relationship between the presence of ESBL phenotype and the distribution of cas genes.     Manuscript profile
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  8 - Frequence of ESBLs and Antibiotic Resistant Pattern in to E.coli and K.pneumoniae Strains Isolated of Hospitalized and Out patients Acquired Urinary Tract Infection (Esfahan/2008-2009)
  Shilla Jalalpoor Sina Mobasherizadeh
  Introduction and Objectives: Escherichia coli and Klebsiella pneumoniae are the most agent of urinary tract infection (UTI) and prevalence Extended-Spectrum Beta-lactamase (ESBLs) in these bacteria due to spread of antibiotic resistance and mortality and morbidity in pa More

  Introduction and Objectives: Escherichia coli and Klebsiella pneumoniae are the most agent of urinary tract infection (UTI) and prevalence Extended-Spectrum Beta-lactamase (ESBLs) in these bacteria due to spread of antibiotic resistance and mortality and morbidity in patients. The best manner for control of ESBLs in bacteria, are inhibition of spread these bacteria and use of standard method for recognizes ESBLs producer strains. Subject of this study was comparison frequency of ESBLs in Escherichia coli and Klebsiella pneumonia strain in UTI acquired patients with phenotypic test. Materials and Methods: This cross-sectional search was performed in Azzahra and Shariaty hospitals during of 2008-2009 years in Esfahan, according to statistical formula randomly selected 91 samples from urinary infections. Bacterial identification was performed with microbiological methods, ESBLs production was performed with screening and confirmatory test and survey antibiotics resistant pattern was performed with Kirby method. Results: Frequence of ESBLs in E.coli and K.pneumoniae strains was respectively 47.97% and 41.66% .According to antibiogram result respectively 59.2%, 54.9%, 30.3%, 27.8%, 19.5% and 16.7% of E.coli strains were resistant into Co-Trimoxazole, Nalidixic acid, Ciprofloxacin, Gentamicin, Ceftazidime and Nitrofurantoin and respectively 75%, 50%, 40%, 44.5%,37.5%, 37.5%, 22.3% and 0% of K.pneumoniae strains were resistant into Ampicillin, Co-Trimoxazole, Nitrofurantoin, Ceftazidime, Amikacin, Cephotaxime, Imipenem and Ciprofloxacin. Conclusion: The result showed high frequence of ESBLs, so  antibiotic resistant in isolated bacteria from hospitalized into out patience's that represent high spread  antibiotic resistant strains in hospitals. Manuscript profile