• List of Articles Dextran

      • Open Access Article

        1 - Chemical Modification of Lysozyme with Dextran by Using Maillard Reaction and Evaluating the Antimicrobial Properties of the Modified Enzyme
        Mahmood Aminlari Roghayeh Ramezani sedigheh amiri
        Introduction: Lysozyme is a natural enzyme with positive antimicrobial activity against Gram Positive  bacteria, but its action is limited on Gram Negative bacteria, which is assumed a restriction for its uses in industry. The purpose of this research was to glycos More
        Introduction: Lysozyme is a natural enzyme with positive antimicrobial activity against Gram Positive  bacteria, but its action is limited on Gram Negative bacteria, which is assumed a restriction for its uses in industry. The purpose of this research was to glycosylate lysozyme with dextran through Maillard-based reaction and to study the antimicrobial characteristics of lysozyme-dextran conjugate.Materials and Methods: Glycosylation of lysozyme with dextran was performed using a 1:5 weight ratio of protein to dextran, incubated at 60 MC for one week under the relative humidity of 79%. For evaluating conjugation of dextran to lysozyme, SDS-PAGE electrophoresis was conducted. Gel filtration chromatography with sephadox G-100 was used for separation of conjugated enzyme. Lytic activity, free amino group and antimicrobial activity of the modified enzyme were evaluated.Results: SDS-PAGE electrophoresis was used to follow the glycosylation process. Results indicated that 3.7 moles of dextran were coupled to one mole of lysozyme. The lytic activity of the conjugate was about 62% of that of the native lysozyme. Evaluation of antimicrobial activity of the lysozymedextran conjugate, indicated the effectiveness of modified enzyme against E. coli and a progressive increase in antimicrobial activity with an increase in enzyme-conjugate concentration. The antimicrobial action of lysozyme on S. aureus was not improved by conjugation with dextran as compared with that of E. coli.Conclusion: These results might increase the application of lysozyme as a natural antimicrobial ingredient in different food systems. Manuscript profile
      • Open Access Article

        2 - A Comparative Study Concerning Qualitative Characteristics that Influence the Refining Operation of Raw Imported Sugar and the Sugar Produced Domestically
        M. E Bahrami مسعود Honarvar
        of syrup. Raw cane sugars are most widely used and are important as raw materials in thesugar industry. The purpose of this study is to evaluate and compare the quality of raw canesugar and the factors influencing and controlling the refining process and compare the res More
        of syrup. Raw cane sugars are most widely used and are important as raw materials in thesugar industry. The purpose of this study is to evaluate and compare the quality of raw canesugar and the factors influencing and controlling the refining process and compare the resultswith the domestically produced sugars.Materials and Methods: This study evaluates the physical and chemical properties such asmoisture, ash, sucrose content, pH, refractive index, colour, invert content, dextran and starchconcentrations in the raw sugar.Results: The result indicated that the imported samples of raw sugars had higherconcentration of sucrose but lower percentages of invert, dextran, colour and ash as comparedto the domestic samples. This might affect the cost of the refining operation. It is worth tomention that the imported raw sugars had generally higher starch concentrations than thedomestic samples.Conclusion: The imported samples with high polarization and low impurities arerecommended to produce the white sugar. Manuscript profile
      • Open Access Article

        3 - One-step cathodic electro-synthesis and characterization of dextran coated magnetite nanoparticles
        Mustafa Aghazadeh Isa Karimzadeh
      • Open Access Article

        4 - One-step Cathodic Electrochemical Synthesis and Characterization of Dextran Coated Magnetite Nanoparticles
        Mustafa Aghazadeh Isa Karimzadeh
      • Open Access Article

        5 - Effect of dextran prebiotic on experimentally induced Coccidiosis in broilers (Ross Strain) by Lesion scoring technique
        S. S. R Shojaei حامد Hasani Valasejerdi کسری Esmaeilnia
        Cocciodiosis is one of the most important parasitic diseases of poultry that is caused by protozoa of the genuseimeria. Due to increasing regulations with the use of prophylactic drugs, high cost of vaccines, and escalatingconsumer’s interest on naturally-raised c More
        Cocciodiosis is one of the most important parasitic diseases of poultry that is caused by protozoa of the genuseimeria. Due to increasing regulations with the use of prophylactic drugs, high cost of vaccines, and escalatingconsumer’s interest on naturally-raised chickens, much interest has been devoted toward the development of alternative strategies to control avian coccidiosis. For example, prebiotics can be useful. Dextran, which is a glucosepolymer and has recently been under investigation as a prebiotic. dextran can promote the growth of benefcallactic acid bacteria in the intestines and boosting immune system, it can be speculated that, this effect promotes thehealth and productivity of poultry. There are not any studies about effect of this prebiotic on poultry coccidiosis.The hypothesis tested was that the feeding of dextran will suppress the lesion scores of a coccidiosis infectionin broilers. To evaluate the effect of dietary dextran, a total of One hundred and twenty 1-day old broiler chicks(Ross 308) were purchased from a local hatchery and randomly allocated to 6 groups of 20 birds each. Four groupsreceived the supplemented diet with dextran (experiment groups) and the other two groups were fed the basal diet(control groups). The experimental diet was prepared by adding a dextran preparation at a level of 1.5 g/kg diet.On day 21, Four groups fed the supplemented diet and one group fed the basal diet were challenged with 4 dilutedmixture of Eimeria containing sporulated oocysts of E. acervulina, E. maxima, E. tenella and E. necatrix. On day27 0f the experiment, 4 birds per group, were randomly selected, euthanized by cervical dislocation. The intestinewas removed, opened and coccidial intestinal lesion scored. The results of this experiment showed the mean oflesion score did differ signifcantly between the infected birds fed the diets without or with dextran in same dosesof sporulated oocyst inoculation. Manuscript profile