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        1 - Prevalence of Cryptosporidium and Giardia in rural herd and guard dogs of Mahabad region
        Ali Hayatrouhi Mohammad Sadaghian
        The genera Cryptosporidium and Giardia are among the most common protozoal parasites causing small intestinal abnormalities of dogs.  In this study, 200 fecal samples of herd and guard dogs were collected randomly from different rural areas around Mahabad city and More
        The genera Cryptosporidium and Giardia are among the most common protozoal parasites causing small intestinal abnormalities of dogs.  In this study, 200 fecal samples of herd and guard dogs were collected randomly from different rural areas around Mahabad city and referred to the laboratory after stabilization with formalin and polyvinyl alcohol solutions. Samples were microscopically studied using concentration method followed by the Trichrome-Gumurian and modified Ziehl-Neelsen staining techniques. In general, 9 (4.5%) and 31 (15.5%) of the studied dogs were infected with Cryptosporidium and Giardia respectively. The highest incidence of cryptosporidium was observed in dogs less than 6 months (10.43%) and the lowest incidence was in the age group more than 4 years (1.54%). The highest levels of Giardia infection were found in the 6-12 months age group (25%) and the lowest in the 1-4 years age group (8.62%). The prevalence of infection with cryptosporidium and giardia in male dogs was 4.67% and 14.95%, respectively, and 4.30% and 16.13% in female dogs, respectively. The prevalence of both protozoa in male and female dogs as well as in different age groups was not statistically significant. There were no significant relation between age and gender with prevalence of both parasites. However, the level of infection with both Cryptosporidium and Giardia protozoa in dogs with inappropriate sanitation was higher than those with proper sanitary status, but this difference was not statistically significant. Manuscript profile
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        2 - A survey on Cryptosporidium spp. infection in lambs with and without clinical signs of diarrhea in Jovein area
        Robabe keyvanloo Shahrestanakey Alireza Taghavi razavizadeh Gholamreza Razmi
        Cryptosporidium parvum is an enteric pathogen of a broad range of animals including ruminants. It is also important in terms of public health due to its transmission from animals to humans. The purpose of the present study was to determine the infection rate of this par More
        Cryptosporidium parvum is an enteric pathogen of a broad range of animals including ruminants. It is also important in terms of public health due to its transmission from animals to humans. The purpose of the present study was to determine the infection rate of this parasite in lambs raised in farms of Jovein area as a center of sheep production in khorasan-razavi province. At first, some sheep flocks were selected randomly, then fecal samples were collected from the rectum of 300 lambs with the age of 10 days to two months during four seasons between summer 1393 to spring 1394 (July 2014 to May 2015). After recording the sampling time, age, sex, and presence or absence of clinical signs of diarrhea in studied lambs, the samples were transported to the parasitology laboratory. Smears were prepared and stained by modified Ziehl-Neelsen method and examined by light microscopy using magnification of 100× (oil immersion). The results of 150 cases of diarrheic and 150 cases of non- diarrheic stools showed that the infection rate is 3% (9 positive samples). Two (1.3%) and 7 (4.6%) positive samples belonged to diarrheic and non- diarrheic lambs, respectively. The Chi-square test revealed no statistically significant correlation between the protozoan infection rate with diarrhea, age, sex and season of sampling. Although the cryptosporidium infection rate in lambs were relatively low in Jovein area, but sheep should not be overlooked as a possible source of infection that could threaten public health.   Manuscript profile
      • Open Access Article

        3 - Survey of Cryptosporidium parasite infection in poultry farms around Tabriz
        hossein hashemzade farhang parisa Shahbazi reza jafari
           Cryptosporidium is a genus of apicomplexan protozoa that can cause enteric infection or respiratory form of infection with up to 10% mortality in broiler flocks  .In the respiratory forms up to 50% of a flock may show clinical signs. In addition to the More
           Cryptosporidium is a genus of apicomplexan protozoa that can cause enteric infection or respiratory form of infection with up to 10% mortality in broiler flocks  .In the respiratory forms up to 50% of a flock may show clinical signs. In addition to the direct economic effects of the clinical disease, the damage caused to the immune system and interaction with other pathogens such as IBD virus has significant effects.  In the present study, 400 fecal samples from 20 broiler farms were prepared and examined. Based on our results, cryptosporidium infection was seen in 2 of 20 studied farms. Infection rate in two farms were 40% and 65% respectively. According to these results and other studies, cryptosporidium infection could be an important problem in poultry farms and it is necessary to carry out further studies about cryptosporidium infection in poultry farms. Manuscript profile
      • Open Access Article

        4 - The survey on Cryptosporidium parasite infection in Tabriz and suburb native poultry
        hossein hashemzadefarhang parisa shahbazi seyyed razi bahavarnia
           Cryptosporidium is a genus of apicomplexan protozoans that can cause gastrointestinal illness with diarrhea in domestic animals, poultry and humans. In addition to the direct economic effects of the clinical disease, the damage caused to the immune system a More
           Cryptosporidium is a genus of apicomplexan protozoans that can cause gastrointestinal illness with diarrhea in domestic animals, poultry and humans. In addition to the direct economic effects of the clinical disease, the damage caused to the immune system and interaction with other pathogens such as IBD virus has significant effects. The present study has been carried out with the purpose of determining infection to Cryptosporidium.in native poultry Tabriz region and its suburb the present study, 400 fecal samples from native poultry were prepared and examined. Based on our results, from 400 examined samples in 14% of cases cryptosporidium infection was seen. The highest infection vote with 15 positive samples (15%) was recorded during winter and the next highest infection with 9% was seen in autumn. Chi squared analysis of results indicated significant difference infection rate during difference seasons of the year (p<0/05). There was no significant difference in infection rate between malls and females. According to our results, native poultry cryptosporidium infection rate in Tabriz and suburb is high and this problem could be related to native poultry husband any methods and their free unhealthy nutrition and drinking in the environment. Manuscript profile
      • Open Access Article

        5 - A study on protozoan infections (Giardia, Entamoeba, Isoapora and Cryptosporidium) in stray dogs in Ilam province
        سیامک Kakekhani A.M Bahrami سلمان Ahmadai Asbchin علی Doosti
        Giardia, Entamoeba, Isospora and Cryptosporidium are important protozoan parastites that caused diarrhea in human and animals. In the present study, fecal samples were collected fresh, directly from the rectum of 112 stray dogs in Ilam province. Giardia and Entamoeba we More
        Giardia, Entamoeba, Isospora and Cryptosporidium are important protozoan parastites that caused diarrhea in human and animals. In the present study, fecal samples were collected fresh, directly from the rectum of 112 stray dogs in Ilam province. Giardia and Entamoeba were concentrated by using the formalin ether sedimentation method followed by the trichrome and iodine staining technique andCryptosporidium  oocysts  were  concentrated  by  using  the  formalin  ether  sedimentation  method  followed by the modified Ziehl-Neelsen staining technique. Of 112 stray dogs, protozoan infections were detected from feces of 46 dogs (41.07%) that Giardia infection was detected from feces of 21 dogs (18.75%), Isospora 17 (15.17%), Cryptosporidium 8 (7.14%) and synchronization infection to 2 protozoan in 9 dogs (8.03%) and to 3 protozoan in 3 (2.67%). In the present study not observed to Entamoeba. No statistically significant differences in prevalence of protozoan parasites occurred between female (34.21 %) and male (55.5 %) stray dogs (p>0/05). But statistically significant differences in prevalence occurred between 1≥0 and 0 ≥1 stray dogs (p>0/05). So that stray dogs of Ilam province can cause infection of human water and food sources.   Manuscript profile
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        6 - A Survey on frequency of equine Cryptosporidiosis in Ahvaz
        A.R Ghadrdan-Mashhadi حسین Hamidienjat پریسا Alizadehnia
        Cryptosporidia are protozoan parasites that have worldwide distribution and can cause diarrhea and mal-absorption in young horses. Sometimes, it can cause illness in neonatal foals, too. In present study, the frequency of equine Cryptosporidiosis in Ahvaz was investigat More
        Cryptosporidia are protozoan parasites that have worldwide distribution and can cause diarrhea and mal-absorption in young horses. Sometimes, it can cause illness in neonatal foals, too. In present study, the frequency of equine Cryptosporidiosis in Ahvaz was investigated. The samples were taken from 100 horses during spring and summer 2009. The ages of horses were from 1 month to 27 years old. Fecal samples were stained by modified Ziehl-Neelsen method. Results were analyzed with Chi-square Test and Fisher’s exact test. The results showed that the rate of infection to Cryptosporidium was 18%. There was no significant correlation between infection to Cryptosporidium and sex, age and fecal thickness. The rate of infection to Cryptosporidium in studied horses is very similar to studies done in other countries. Manuscript profile
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        7 - The Efficiency of Water Supply Facilities Susangerd the Removal of Giardia and Cryptosporidium Parasites
        Mohammadjavad Saeedifar Abdolkazem Neisi
        The aim of this study was to determine the efficiency of water treatment plants and water supply network Susangerd remove parasites Giardia lamblia (Giardilamblia) parvum and Cryptosporidium (Cryptosporidium parvum), respectively. The total number of samples taken on th More
        The aim of this study was to determine the efficiency of water treatment plants and water supply network Susangerd remove parasites Giardia lamblia (Giardilamblia) parvum and Cryptosporidium (Cryptosporidium parvum), respectively. The total number of samples taken on the basis of the population of 16 samples was 0.400 liters of water through the filter of 5 microns. The 5 micron filter mud samples collected from different areas in the laboratory by laboratory instruments, including brushes, spoons, sterile, man, Arlene was separated and distilled water. After sedimentation and centrifugation by Lam Sdvyk Raftr and parasites by microscopy to view Aynvryt were counted. Tests pH, turbidity, total dissolved solids, residual chlorine and fecal coliform were performed according to standard procedures in 1011. The average number of counted Lamylya Giardia lamblia and Cryptosporidium parvum in drinking water distribution Susangerd 3 Dddr Lytrbvd 9 per liter. Average turbidity and chlorine residual distribution network NTU6.6 network mg / l 8/0 and utilities output remains Klrbaqy 1.2mg / l respectively. The study showed that water treatment plants and water supply network in the removal of Giardia and Cryptosporidium Susangerd Parvvmbdlyl see these parasites in the water supply network and distribution network did not work Manuscript profile
      • Open Access Article

        8 - Survey on Prevalence Rate of Cryptosporidiosis in Sheep and Goats Population of Mahabad City
        Kiarash Dezhban Sohrab Rasouli
        Cryptosporidium is a small coccidian parasite invaded and replicated within the epithelial cell lining of digestive, respiratory, and conjunctiva organs of vertebrates. Cryptosporidium is an intracellular and exocytoplasmic organism. It has been shown to be an important More
        Cryptosporidium is a small coccidian parasite invaded and replicated within the epithelial cell lining of digestive, respiratory, and conjunctiva organs of vertebrates. Cryptosporidium is an intracellular and exocytoplasmic organism. It has been shown to be an important cause of enterocolitis and self-limited diarrhea in numbers of animals with defect in their immunity system. In this survey, we examined 419 fecal samples including sheep and goats population of Mahabad city, Iran. Forty nine samples of this examined animals (17 samples of sheep and goats are under 1 year old, 11 samples of sheep and goats between 1 and 2 years, 9 samples of sheep and goats between 2 and 3 years, 8 samples between 3 and 4 years old, and 4 samples of sheep and goats are over 4 years) are positive with a view of shedding of oocyst. There was significant statistical relationship between infection rate and sex P<0.05. There was significant statistical relationship between infection rate and age P<0.05. There was significant statistical relationship between infection rate and seasons of the year P<0.05 and there was no significant statistical relationship between sheep population and goat population in Mahabad city (P>0.05). Manuscript profile
      • Open Access Article

        9 - Study of Cryptosporidium infection in sheep in Saveh County
        mohammad hasan rabiee alireza shaghayegh shapourreza shojaei
         Cryptosporidium is a parasite that can cause diarrhea in sheep. Disease caused by Cryptosporidium is a zoonotic disease. Given its importance, this study carried out to evaluate the Cryptosporidium infection rate in sheep in Noubaran distinct of Saveh County in 20 More
         Cryptosporidium is a parasite that can cause diarrhea in sheep. Disease caused by Cryptosporidium is a zoonotic disease. Given its importance, this study carried out to evaluate the Cryptosporidium infection rate in sheep in Noubaran distinct of Saveh County in 2014.  In this cross-sectional study on 266 asymptomatic sheep, fecal samples were collected and transported to the laboratory and condensed. then samples were stained using modified Ziehl-Neelsen technique and examined by the use of an optical microscope.  The prevalence of Cryptosporidium infection in studied samples was 10.5%. The results indicate that the rate of infection in rams was higher than ewes, the rate of infection in less than 1 year old sheep was higher than over 1 year old ones, and the rate of infection in herds with over 100 sheep was higher than herds with less than 100 sheep. Of course, no statistically significant relation was seen between factors such as age, gender and the number of herds with disease (p>0.05) This study showed that Cryptosporidium infection in sheep in this area was noticeable. Meanwhile, as this study has been done on animals without clinical signs, asymptomatic sheep could be a potential source to spread the infection to humans and ultimately the community. Manuscript profile
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        10 - survey on prevalence rate of cryptosporidiosis in referring dogs to veterinarian hospitals of Alborz province
        Hossein Mahdavi Fekejvar Sohrab Rasouli omid mohammadpour Esmaeil Minaei
        Cryptosporidium is small coccidian parasites wich invade and replicate within the epithelial cell lining the digestive, respiratory and conjunctival organs of vertebrates.Cryptosporidium is a intracellular and extracyto plasmic organism.it is been shown to be an importa More
        Cryptosporidium is small coccidian parasites wich invade and replicate within the epithelial cell lining the digestive, respiratory and conjunctival organs of vertebrates.Cryptosporidium is a intracellular and extracyto plasmic organism.it is been shown to be an important parasite cause of entrocolitis and selflimited diarrhea in numbers of domestic and pet animals with defect in their immunity system. In this survey we examing 112 fecal samples including referring dogs to veterinarian hospitals in alborz province by Ziehl-Neelsen stain technique.7 samples of this examined animals (3 samples of dogs under 1 year old, 2 samples of dogs between 1 and 2 years, 1 samples of dogs between 2 and 3 years, 1 samples of dogs between 3 and 4 years old) are positive with a view of shedding of oocyst. There was no significant statical relationship between infection rate and sex (P>0.05), but there was significant statical relationship between infection rate and age (P Manuscript profile
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        11 - Detection of Rota virus, corona virus, E.coli (k99), cryptosporidium and salmonella in diarrheic calves (up to one month old) in Tehran province farms
        , F Moosakhani آریا Badiei A.H Asadi حامد Asadi علیرضا Shaghayegh محسن Mozaffari
             The aim of the current study was to estimate the prevalence of five major enteropathogens in under 1 month old diarrheic claves in Tehran province.also, the relationship between season with occurrence these Enteropathogens and comparison the result More
             The aim of the current study was to estimate the prevalence of five major enteropathogens in under 1 month old diarrheic claves in Tehran province.also, the relationship between season with occurrence these Enteropathogens and comparison the results with other geographical regions were evaluated.135 fecal samples from diarrheic calves (under 1 momnth old) collected from 25 farms in Tehran province between April 2010 to June 2011.samples were examined for rotavirus,corona virus,E.coli(k99) and cryptosporidium by using capture Elisa and culturing for samonella spp.salmonella infected samples were serotyped by standard antiserum.Chi-square test was used to determine season association with five enteric pathogens prevalence.totally infection rate from 135 fecal samples , cryptosporidium in 53 samples(39.2%), Rota virus in 43 samples(31.8%),Salmonella spp in 13 samples(9.6%), Corona virus in 12 samples(8.8%) and E.coli (k99) in 8 samples(5.9%) were detected.from 13 salmonella isolated samples,seven of them (5.18%) were typed as salmonella serogroup D and six of them(4.4%) were typed as salmonella serogroup B.the result of this study indicated that cryptosporidium and rotavirus were the most frequently identified pathogens in this study.also season had significant association with salmonella,rota virus and E.coli(k99) prevalence.(p-value<0.05-confident interval (C.I)=95%).     Manuscript profile