Molecular investigation of the frequency of Cryptosporidium parvum in feces of calves with diarrhea in Alborz province
Subject Areas : clinical veterinary science
رضا حسینی
1
,
Milad Hamzehali Tehrani
2
,
Navid Sadeghi
3
1 - استادیار، گروه آموزشی انگل شناسی،دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی،شهرکرد، ایران
2 - گروه پاتوبیولوژی- دانشکده دامپزشکی- واحد علوم و تحقیقات تهران- دانشگاه آزاد اسلامی نهران
3 - Department of Pathobiology, Islamic Azad University, Karaj Branch, Karaj, Iran.
Keywords: Calves of Alborz province, Cryptosporidium parvum, Feces, Karaj, Molecular investigation,
Abstract :
Background: Cryptosporidium is one of the main causes of disease and mortality in young livestock and is also one of the most important intestinal agents in mammals, especially domestic animals and humans. Clinical symptoms in affected animals are diarrhea, malabsorption, weight loss, and milk loss. Due to the zoonotic nature of this parasite, it is very dangerous for human societies. Nowadays, Cryptosiridium is considered as one of the etiological factors of infant diarrhea syndrome in ruminants, especially in calves. It is associated with a gastrointestinal infection or it causes diarrhea independently. Objectives: This study aimed to investigate the molecular prevalence of Cryptosporidium parvum in the feces of calves with diarrhea in Alborz province. Methods: After collecting 52 diarrhea samples from 12 calves from cattle ranches in Alborz province and transferring them to the laboratory, DNA extraction was performed, then PCR was performed using RFLP-PCR method. The test results were analyzed by SPSS software. Results: 7% of the samples out of 52 were infected with Cryptosporidium, after testing and analysis, it was found that 2 were parvum, also the probability of infection with Cryptosporidium parvum in calves under 1 months is more than that of 1 to 6 months of age, but the level is significant Not seen in this case. Conclusions: According to the results obtained in this study and the review of past reports, it shows that the prevalence of infection with Cryptosporidium parvum parasite in the cattle farms of Alborz province is low compared to other parts of Iran. However, due to the joint infection of humans and animals by this parasite, appropriate preventive and therapeutic measures should be taken to eradicate this parasite.
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