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      • Open Access Article

        1 - The Effectiveness of Alcoholic Rosa Damascene Extract on Lactobacilus Acidophilus and bifidobacterium lactis Probiotic Bacteria Survival in Doogh
        , B Akbari-adergani سمیرا Asadolahi fard, AA Anvar , F Shirkhan
        In this research, the effect of Rose alcoholic extract on the viability of probiotic bacteria Lactobacillus acidophilus (La-5) and Bifidobacterium lactis (Bb-12) in Doogh was studied. Probiotic bacteria Bifidobacterium bifidum and Lactobacillus acidophilus were inoculat More
        In this research, the effect of Rose alcoholic extract on the viability of probiotic bacteria Lactobacillus acidophilus (La-5) and Bifidobacterium lactis (Bb-12) in Doogh was studied. Probiotic bacteria Bifidobacterium bifidum and Lactobacillus acidophilus were inoculated into Doogh without Rose alcoholic extract (control) and containing Rose alcoholic extract (1% and 2%). Doogh produced during 4 weeks of storage in a refrigerator at 4° C during a week was investigated for microbial count, pH changes, acidity and survival during the shelf life and the organoleptic characteristics of the product were studied. The results of the statistical analysis indicated that the difference in survival percentage of La-5 and Bb-12 between the ordinary Doogh and the Doogh containing Rose alcoholic extract was significant (p≥0.05). Regarding taste, the results of this study showed that there is a significant difference between the types of Doogh in terms of taste (p<0.05). The study of organoleptic characteristics of Doogh containing Rose alcoholic extract showed that the flavor of this Doogh was improved compared to ordinary Doogh. The pH decreased during the shelf life and the acidity increased slightly. The highest and lowest levels of pH were respectively observed for samples containing 1% and 2% extract. Also, the effect of extract amount on acidity showed that the highest and lowest levels of acidity were in the samples containing 2% and 1% extract, respectively. Therefore, the production and consumption of Doogh containing Rose extract by maintaining organoleptic characteristics together with probiotic bacteria as a pragmatic food product are recommended. Manuscript profile
      • Open Access Article

        2 - Feasibility of vitamin D and Bifidobacterium Lactis loaded-alginate microcapsules for producing probiotic / vitaminated butter
        Sahar Saraghi Peyman Rajaei Kaveh Zargari
        Abstract   One of the new ways to improve probiotic bacteria persistence in food products, as well as the survival of bacteria while passing the digestive tract, is to microbialize the probiotic cells in order to safely transport them to the large intestine.&n More
        Abstract   One of the new ways to improve probiotic bacteria persistence in food products, as well as the survival of bacteria while passing the digestive tract, is to microbialize the probiotic cells in order to safely transport them to the large intestine. The aim of this study was to determine the effect of microsphere on the survival of Bifidobacterium lactis during pasteurized butter storage period. In this study, alginate microcapsules containing Bifidobacterium lactis were used in butter formulation with values ​​of 1, 2, 3 and 4 cc. Morphological, particle size, and survival tests were performed in similar conditions in the intestine and stomach. Pasteurized butter tests also included acidity assessment, peroxide value, anisidine index, oxidation resistance, melting point, survival rate of Bifidobacterium lactis, as well as mold and yeast populations in three replications. Sensory evaluation including (flavor, flavor, flavor, perfume, oral sensation and general acceptance) was performed by ten evaluated trained butterfly factory using a 5-point Hedonic method. The results were analyzed by two-way ANOVA and SAS software version 9.4. Non-parametric Kruskal-Wallis Mann-Whitney test was used to assess sensory results. The results showed that alginic microcapsules with a soft and average surface area had a particle size of 300 to 400 microns, and the maximum amount of bacteria in which the bacteria had fallen was 3/3 ± 0.05. Using alginate microcapsules, the index of peroxide, anisidine index decreased significantly as antioxidant properties of alginate as well as vitamin D, and the index of oxidation resistance as well as acidity significantly decreased. Also, by increasing the amount of micro-capsule application, the melting point of butter treatments decreased and the amount of mold and yeast was significantly decreased. Compared to the pasteurized trifoliate with free Bifidobacterium lactis, micro capsulation improved the Bifidobacterium lactis survival rate by two and a half cycles improved logarithmic conditions. Finally, Pasteurized butter treatment with 3 cc of alginate microcapsules was selected as the optimum treatment. Manuscript profile
      • Open Access Article

        3 - Possibility of the production of probiotic chocolate yogurt
        M. Sharifi Soltani G. Karim R. Pourahmad
        The aim of study was to produce a low-fat cacao containing probiotic yogurt. For this reason a combination of probiotic bacteria, lactobacillus acidophilus and Bifidobacterium lactis (3% w/w) together with 0.8% cacao powder, 5% sugar as a well as 1.2% cacao powder with More
        The aim of study was to produce a low-fat cacao containing probiotic yogurt. For this reason a combination of probiotic bacteria, lactobacillus acidophilus and Bifidobacterium lactis (3% w/w) together with 0.8% cacao powder, 5% sugar as a well as 1.2% cacao powder with 7% sugar were used to produce a synbiotic yogurt (0.5% and 1.5% fat). Populations of probiotics bacteria, physiochemical and sensory properties of the yogurt samples were evaluated throughout the storage period (1, 7, 14, and 21 days). Result showed that cacao as prebiotic could significantly increase the viability of L. acidophilus (P<0.01); besides no significant difference was observed between the populations of B. lactis in the chocolate yogurt and control samples. The highest quantity of the probiotic bacteria was observed at day 7, followed by a decrease in their number; however their populations remained above 7 log cfu/g. Moreover, pH value of the samples decreased over the time of storage, but pH value of the cacao-containing samples increased significantly (P<0.01).In comparison with the control group, no significant increase was recorded in the lipid content of the samples with cacao and sugar, meanwhile the amount of dry matter was higher than control samples (P<0.01). It could be concluded that apart from the prebiotic properties of cacao on L. acidophilus,it can improve the nutritional value of probiotic yogurt and increases the calorie of the product. However for achieving better sensory properties, changes should be considered in the formulation.   Manuscript profile
      • Open Access Article

        4 - The Effect of Extract and Fiber of Mint Stalk on Viability of Bifidobacterium Lactis Bacteria in Stirred Yogurt Using the Response Surface Method
        محسن وظیفه دوست امیر حسین الهامی راد مسعود شفافی زنوزیان محمد حسین حداد خداپرست محمد آرمین
        In today's world, there is a growing tendency towards producing healthy and nutritious food. Mint is a genus of plants that its healthy and nutritious effects have been proved. Adding probiotic bacteria to yogurt increases its nutritious benefits. This study aims at add More
        In today's world, there is a growing tendency towards producing healthy and nutritious food. Mint is a genus of plants that its healthy and nutritious effects have been proved. Adding probiotic bacteria to yogurt increases its nutritious benefits. This study aims at adding the extract and fiber of mint stalk to stirred yogurt containing Bifidobacterium Lactis bacteria (BB-12) as well as examining the viability of this bacterium. To do so, the response surface methodology and the central composite rotatable design were employed. The findings obtained from the analysis showed that there was a significant relationship between different concentrations of fiber and extract, and the viability of Bifidobacterium Lactis bacteria in the first day to the 21st day (P<0.01). Having applied the response surface method, the optimization depicted that the maximum viability of Bifidobacterium Lactis bacteria is only achieved when the concentration of extract and fiber are 0.1 and 1.3 percent, respectively. In these conditions, the utility of the model equals 0.94 and the number of Bifidobacterium Lactis bacteria is 7.11±0.005 log cfu/ml in the first day, 6.49± 0.035 log cfu/ml in the 7th day, 6.24 ± 0.055 log cfu/ml in the 14th day, and 5.41±0.17 log cfu/ml in the 21st. Since the produced yogurt under the condition of optimization contained at least 106 colony of bacteria per milliliter, the said product was called functional. Manuscript profile
      • Open Access Article

        5 - Survival of Lactobacillus acidophilus and Bifidobacterium lactis and quality properties of yogurt made from goat's milk
        Rahele Nezhad Razmjoui Akhgar Shahin Zomorodi
        Goat's milk is a proper alternative to cow's milk because of its higher digestibility and lower allergic characteristics, as well as its higher functional nutrient. On the other hand, its health benefits can be increased by the incorporation of probiotics into goat's mi More
        Goat's milk is a proper alternative to cow's milk because of its higher digestibility and lower allergic characteristics, as well as its higher functional nutrient. On the other hand, its health benefits can be increased by the incorporation of probiotics into goat's milk. This research aimed to investigate the survivability of Lactobacillus acidophilus and Bifidobacterium lactis (B. lactis) individually and in combination on the physicochemical and sensory properties of yogurt made of goat's milk during 21days storage. In this research, 4 treatments were prepared, including control (with no probiotic bacteria), La treatment containing Lactobacillus acidophilus, Bb treatment containing B. lactis, and LaBb treatment containing a combination of Lactobacillus acidophilus and B. lactis. The results showed that the count of Lactobacillus acidophilus, B. lactis, and their combination reached from 8.54 log cfu/g on the first day to 9.43, 10.06 and 10.75 log cfu/g at the end of the storage period, respectively. At the end of the storage period, the control sample had significantly the highest pH and syneresis and the lowest acidity, water holding capacity, viscosity, and overall acceptability among treatments. However, no significant difference between samples containing probiotic in acidity, pH, viscosity, syneresis, water holding capacity, and overall acceptability was observed. According to the results obtained of this study, yogurt made of goat's milk, in addition to being a suitable carrier for probiotics, its sensory properties improve as a result of the incorporation of these probiotics. Manuscript profile
      • Open Access Article

        6 - Detoxification of aflatoxin M1 by Bifidobacterium lactis and Streptococcus thermophilus in skimmed milk
        Masoud Atashpanjeh Seyed Amirali Anvar Amireghbal Khajehrahimi Maryam Tala نکیسا  Sohrabi Haghdoost
        Contamination of food products, especially dairy products, with aflatoxins, is one of the main problems in the food industry. This research aimed to remove aflatoxin M1 using two probiotics, Bifidobacterium lactis and Streptococcus thermophilus, either alone or in combi More
        Contamination of food products, especially dairy products, with aflatoxins, is one of the main problems in the food industry. This research aimed to remove aflatoxin M1 using two probiotics, Bifidobacterium lactis and Streptococcus thermophilus, either alone or in combination, in skimmed milk containing this toxin. The study investigated the effects of storage time, microbial strain, bacterial concentration, toxin concentration, and temperature on the removal of aflatoxin M1. Two probiotic bacteria, Bifidobacterium lactis and Streptococcus thermophilus, at dilutions of 108 and 1010 CFU/ml, were inoculated alone and in combination into skimmed milk contaminated with different concentrations of aflatoxin M1 (0.1, 0.25, and 0.5 μg/ml). The samples were then incubated at 4°C and 37°C for 0.5, 1, 2, and 24 hours. The detoxification percentage of aflatoxin M1 was measured using high-performance liquid chromatography (HPLC). The results showed an increase in the removal of aflatoxin M1 with time. The removal of aflatoxin M1 by these strains varied from 12% to 87% depending on the concentration of bacteria, storage time, toxin concentration, and bacterial strain, whether alone or in combination. The results of this study suggest that using probiotics can be an effective method for reducing or eliminating the concentration of aflatoxin M1 in the dairy industry. Manuscript profile