Drought is the most important non-biotic stress which decreases growth and the yield of crop plants. Quinoa is a plant with high nutritional value and drought tolerant. To evaluate the effect of cytokinin foliar application on morphological traits and yield of quinoa cu More
Drought is the most important non-biotic stress which decreases growth and the yield of crop plants. Quinoa is a plant with high nutritional value and drought tolerant. To evaluate the effect of cytokinin foliar application on morphological traits and yield of quinoa cultivars, a split factorial experiment based on randomized complete block design was conducted during during 2017 cropping season with four replications at the research farm of Agriculture Faculty, the University of Zanjan. Experimental treatments were levels of irrigation (optimal irrigation, with -0.4 MPa and drought stress with -1.5 MPa) as main factor and sub factors were four quinoa cultivars (Q 26, Q 29, Giza1 and Red Carina) and foliar cytokinin with three levels (0, 50 and 100 μM). Irrigation and cultivar treatment had significant effect on all evaluated traits. Foliar application with cytokinin had significant effect on all traits except length of main ear and greeness index (SPAD). The maximum and minimum of grain yield 2364.7 and 1701.8 kg.ha-1, obtained under optimal irrigation and drought stress conditions, respectively. Drought stress reduced seed yield by 28 percent. The highest yields obtained by Giza 1 (2746.7 kg.ha-1) and Red Carina (2589.7 kg.ha-1) with the application 100 µm of cytokinin. The foliar application of cytokinin did not affect seed yields of Q 26 and Q 29 cultivars significantly. According to the results, Giza 1 can be recommended as a suitable cultivar under both optimum and drought stress conditions at the experimental site.
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This study evaluates the effects of foliar application of auxins and cytokinins hormones on growth and photosynthetic pigment content in Adenium obsum. Experiments were performed in a completely randomized blocks with 8 treatments and a control group, 4 replications, an More
This study evaluates the effects of foliar application of auxins and cytokinins hormones on growth and photosynthetic pigment content in Adenium obsum. Experiments were performed in a completely randomized blocks with 8 treatments and a control group, 4 replications, and 8 stages of hormone application. Two types of auxin, IAA and IBA, 200 mg / l and of cytokinin, BAP and KIN, 400 mg / lit were used independently and in combination. The evaluated traits included leaf area, number of lateral buds and flowers, shoots and roots fresh and dry weight, chlorophyll a and b and carotenoids content. Different treatments containing BAP as well as KIN and IAA + KIN increased leaf area. Highest increase was related to IAA + BAP. The application of hormonal treatments did not have a significant effect on shoot length, except for IBA and IBA + BAP treatments but other treatments increased root length. IBA + BAP and especially IAA + BAP treatment caused a significant increase in the number of lateral buds. Stem fresh weight also increased significantly in IBA + BAP, IAA + BAP and IAA treatments, but stem dry weight increased only in IAA + BAP. The treatments had no effect on root fresh weight but its dry weight increased in all treatments except IAA and IBA + KIN.The highest number of flowers was obtained without significant difference with the control in IBA + KIN treatment. Independent or combined BAP treatments reduced the number of flowers. The content of chlorophylls a and b increased under IAA + BAP and KIN-treated treatments. IBA + BAP also increased chlorophyll b content. Carotenoid content was not affected by treatments. Morphologically, IAA + BAP clearly affected the shape of the leaves, the appearance of the veins and the general shape of the plant.
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The influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘Bianca’ and ‘El Torro’. Third, fourth and fifth nodal explants were cultured on the modified MS medium supplemented with 1. More
The influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘Bianca’ and ‘El Torro’. Third, fourth and fifth nodal explants were cultured on the modified MS medium supplemented with 1.0 and 5.0 mg/l BA. In both the cultivars, higher rate of proliferation (‘Bianca’ 3.75; ‘El Torro’ 2.65) were obtained from the explants distal to the apex than those of the proximal position with 5.0 mg/l BA. But proliferating shoots derived from the fifth nodal explant with 1.0 mg/l attained highest shoot length (‘Bianca’ 1.43 cm; ‘El Torro’ 1.19 cm) and produced higher number of leaves (‘Bianca’ 5.45; ‘El Torro’ 6.30) and fresh weight (‘Bianca’ 659.38 mg; ‘El Torro’ 255.95 mg) per explant than the third and fourth nodal explants. The fifth nodal explant with 1.0 mg/l BA was found the best treatment for the shoot regeneration of rose cvs. ‘Bianca’ and ‘El Torro’.
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Cadmium is a heavy metal that it’s poisonous forms can naturally exist in soil or can enter to soil in following ways:by industrial activities,mine probing and agricultural fertilizers.Phaseolus vulgaris L.is one of the plants that can tolerate poison of cadmium a More
Cadmium is a heavy metal that it’s poisonous forms can naturally exist in soil or can enter to soil in following ways:by industrial activities,mine probing and agricultural fertilizers.Phaseolus vulgaris L.is one of the plants that can tolerate poison of cadmium and Bean is one of the most important species in Fabaceae family that have high economic significance in human nutrition.Regeneration by tissue culture is one of the effective and appropriate methods for preserving and also propagation of plants.In this study,research of effects cadmium nitrate and growth regulators in Phaseolus vulgaris L.tissue culture,percentage of germination,rhizogenesis and callus generation of seed of Bean plant cultivar(cos 16)in mediums containing cadmium nitrate and2ip hormone evaluated.In the Beginning seeds were surface sterilized respectively with:etanol70%,distilled water and sodium hypocholorite5%.Sterilized seeds were cultured into different culture containing:T0(MS),T1(MS+ 2ip: 0.1 mg⁄l );T2(MS + 2ip: 0.1mg⁄l +CdNo3:0.05mg⁄l);T3( MS+ CdNo3: 0.05mg⁄l)for percentage of germination.Explants of leaf and hypocotyl were prepared from ten-day sterilized seedling cultured in T0 medium and moved in T0(MS),T1(MS+2ip: 3mg⁄l );T2(MS+2ip: 5mg⁄l).A number of hypocotyl explants were transferred intoT1(MS+ NAA: 0.5mg⁄l),T2 (MS+ BAP:2.5mg⁄l) ;T3(MS+NAA:0.5mg⁄l+BAP:2.5mg⁄l). Eventually calluses of hypocotyl explant subcultured fromT0 medium into T0(MS), T1(MS+CdNo3:0.05mg⁄l);T2(MS+CdNo3: 0.1mg⁄l).According to the above results,maximum and minimum percentage of germination in T0(MS),T3(MS+CdNo3: 0.05mg⁄l).Best percentage of callus generation inT0(MS),was observed in hypocotyl explants.Also most rhizogenesis inT2(MS+2ip:5 mg⁄l)in leaf explant and Best callus generation and rhizogenesis were seen inT3(MS+NAA: 0.5 mg⁄l +BAP:2.5mg⁄l)in hypocotyl explant.Finally In subculture of the callus of hypocotyl explant,the most callus generation and rhizogenesis was observed in absence of cadmium nitrate:T0(MS).
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