List of Articles Bacteriophage

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Isolation and screening of marine bacteriophages for use in the prevention of Vibriosis in Western white shrimp
  Samira Zarei Nima bahador maryam Mirbakhsh Mohammad Khalil pazir
  The aim of this study was to screen Vibrio species off the coast of Bushehr province and control this shrimp pathogen using phage.For this study, water was sampled in 1398 from areas close to the coast of Bushehr province. Bacteria that cause Vibriosis were determined u More

  The aim of this study was to screen Vibrio species off the coast of Bushehr province and control this shrimp pathogen using phage.For this study, water was sampled in 1398 from areas close to the coast of Bushehr province. Bacteria that cause Vibriosis were determined using biochemical tests and molecular sequencing. Bacterial selection was performed according to the results of antibiogram profiles of dominant bacteria and pathogens. TCBS was used to determine the phage titer of the culture medium and the plugs were counted 24 hours after incubation at 30 ° C.5 bacteria (Vibrio alginolyticus strain SeqID, Vibrio xuii strain Z-03Phage, Vibrio alginolyticus SZ / 10 gene, Vibrio rotiferianus strain SRPR-Phage, Vibrio harveyi strain Z05 were isolated from Bushehr wastewater contaminated waters). Vibrio alginolyticus strain SeqID was selected as the dominant bacterial species according to the results of antibiogram profile determination, growth halo diameter and bacterial biochemical test of the studied species. Based on the results of 16srDNA molecular analysis, the isolated strain of the bacterium was 100% similar to Vibrio alginolyticus. Based on the results of the host domain, Vibrio alginolyticus strain Samira Phage was able to form plugs.The isolated bacteriophage Vibrio alginolyticus strain Samira Phage had a bactericidal effect with a wide range against Vibrio bacteria identified from the shores of Bushehr, which indicates that this bacteriophage can be a good candidate for use in phage therapy Be against viberosis.Keywords: Isolation and screening, Bacteriophage, Vibriosis, Western white shrimp Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - Phage therapy and its application in plant diseases control
  Fatemeh Samiei
  Bacteriophage refers to viruses that specifically infect bacteria.that are mainly used in medicine and veterinary medicine and have recently been considered for control diseases. The use of phages in the biocontrol strategy of plant pathogens is due to their ability t More

  Bacteriophage refers to viruses that specifically infect bacteria.that are mainly used in medicine and veterinary medicine and have recently been considered for control diseases. The use of phages in the biocontrol strategy of plant pathogens is due to their ability to kill bacteria, as well as the observation of phages in the same bacterial host environment, the possibility of being combined with other bactericides and having a relatively low price. Although there are considerable doubts about the use of bacteriophages as effective biological control agents But the emergence of antibiotic-resistant bacteria and possibility of transferring antibiotic resistance to human pathogens and the emergence of copper-tolerant strains among plant bacteria have led to a renewed desire to control disease based on bacteriophages . So far, phage therapy has been successfully used against agents such as bacterial spot of mushrooms, beans leaf spot,soft rot , but the advantage of phages over antibiotics for treating pathogens is their ability to mutate . The major problem of phage-based biological control is the conversion of successful laboratory experiments into effective control methods against the pathogen at the field level. For this purpose, it is necessary to gain a complete understanding of the ecology and the complex host-phage interaction in different plant environments in order to maximize the use of phages as a biocontrol method. In this article, an attempt has been made to review the potential of bacteriophages in the biological control of plant pathogens and introduce its advantages and challenges Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  3 - Phage therapy and its application in plant diseases control
  Fatemeh Samiei
  10.30495/plant.2022.701982
  Bacteriophage refers to viruses that specifically infect bacteria. Bacteriophages are mainly used in medicine and veterinary medicine and have recently been considered for the control of plant diseases. The use of phages in the biocontrol strategy of plant pathogens is More

  Bacteriophage refers to viruses that specifically infect bacteria. Bacteriophages are mainly used in medicine and veterinary medicine and have recently been considered for the control of plant diseases. The use of phages in the biocontrol strategy of plant pathogens is due to their ability to kill bacteria, as well as the observation of phages in the same bacterial host environment, which indicates their ability to survive in the same host environment. Phages have been used as a part of the integrated management of diseases due to their easy and convenient use, the possibility of being combined with other bactericides or alternating use with pesticides, and having a relatively low price. Although there are considerable doubts about the use of bacteriophages as effective biological control agents, the emergence of antibiotic-resistant bacteria and the concern about the possibility of transferring antibiotic resistance from plant pathogens to human pathogens and the emergence of copper-tolerant strains among plant bacteria have led to a renewed desire to control disease based on bacteriophages in modern agriculture. So far, phage therapy has been successfully used against agents such as bacterial spot of edible mushrooms (Pseudomonas tolasi), bacterial leaf spot of mango beans, soft rot caused by Pectobacterium species, apple and pear blight (Erwinia amylovora), potato scab (Streptomyces scabies), geranium bacterial blight (Xantomonas hortorum pv. Pelargonii), tomato bacterial spot (Xantomonas campestris pv. vesicatoria) have been used. It is true that bacteria can become resistant to phages in a manner similar to that seen in antibiotics, but the advantage of phages over antibiotics for treating pathogens is their ability to mutate and infect new hosts. The major problem of phage-based biological control is the conversion of successful laboratory experiments into effective control methods against the pathogen at the field level. For this purpose, it is necessary to gain a complete understanding of the ecology and the complex host-phage interaction in different plant environments in order to maximize the use of phages as a biocontrol method. In this article, an attempt has been made to review the potential of bacteriophages in the biological control of plant pathogens and introduce its advantages and challenges Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  4 - Isolation Alicyclobacillusacidoterrestris specific bacteriophage, the causative agent of fruit juice spoilage, from soil
  Amir TajBakhsh S.Elham Rezatofighi Hossein Motamedi Solmaz Rafiae
  Abstract Alicyclobacillusacidoterrestris is the main bacterial agent of fruit juice spoilage and the methods for its elimination are much paid attention to. Recently, using this phage for controlling bacteria in food products has received special attention. The aim of More

  Abstract Alicyclobacillusacidoterrestris is the main bacterial agent of fruit juice spoilage and the methods for its elimination are much paid attention to. Recently, using this phage for controlling bacteria in food products has received special attention. The aim of this study was to isolate the specific bacteriophages of A. acidoterrestris from soil. In this study, the samples collected from the soil around the Cedruslibani (Cedar) trees were investigated after being centrifuged and filtered to seek the understudied phage. Bacterial culture equal to0.5 McFarland turbidity, isolated from fruit juice and confirmed by molecular and biochemical techniques, was prepared in AAB. Adding a certain amount of environmental samples allowed the phage to be replicated in the environmentcontainingbacteria. Then, the environment was centrifuged and the supernatant, which is the source of the phage, was filtered. The pure phage suspension was assessed in double agar method for studying the formation, shape and size of plaques. Plaque formation confirmed the presence of bacteriophages in the filtrate. Consequently, the heat and acid resistant bacteriophage was isolated from the soilaround the Cedruslibani (Cedar) trees. The results revealed the presence of specific bacteriophage of A. acidoterrestris. This phage can be used for eliminating of this bacterium in fruit juice. Stability of these bacteriophages in fruit juices its specific. Preserving the fruit quality side is of advantages of using this bacteriophage comparing to other methods. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  5 - Shigella sonnei bio-control in chicken meat by the application of a specific bacteriophage
  Khashayar Shahin Majid Bouzari Ran Wang
  Shigella sonnei is the main agent associated with shigellosis in developed as well as developing countries. Finding alternative methods to control Shigella  transmission (foods, drinking water and person to person contact) seems necessary due to the appearance of m More

  Shigella sonnei is the main agent associated with shigellosis in developed as well as developing countries. Finding alternative methods to control Shigella  transmission (foods, drinking water and person to person contact) seems necessary due to the appearance of multidrug-resistant strains among Shigella spp. This study aimed to evaluate the potential of vB_SsoS-ISF002 phage in bio-controlling of S. sonnei in food products. Changes in the S. sonnei (ATCC 9290) count, as well as the propagation of its specific bacteriophage (vB_SsoS-ISF002), were measured in two trial treatment and prevention phases for a period of 144 hours. The phage (108 PFU/g) was added to raw or cooked chicken breast after (in treatment phase) or before (in prevention phase) inoculation with S. sonnei at 2, 24 or 48 h. vB_SsoS-ISF002 phage was capable to reduce S. sonnei growth by at least two logs of viable in both treatment and prevention phases. It was shown that vB_SflS-ISF001 phage has a high potential to be used as a non-chemical preservative and the bio-controlling agent against S. sonnei contamination in the food industry. Manuscript profile