Isolation and screening of marine bacteriophages for use in the prevention of Vibriosis in Western white shrimp
Subject Areas :Samira Zarei 1 , Nima bahador 2 , maryam Mirbakhsh 3 , Mohammad Khalil pazir 4
1 - Department of Microbiology, College of Science, Shiraz Branch, Islamic University, Shiraz, Iran
2 - Department of Microbiology, College of Science, Shiraz Branch, Islamic University, Shiraz, Iran
3 - Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
4 - Iranian Shrimp Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bushehr, Iran.
Keywords: Western white shrimp, Vibriosis, Isolation and screening, Bacteriophage,
Abstract :
The aim of this study was to screen Vibrio species off the coast of Bushehr province and control this shrimp pathogen using phage.For this study, water was sampled in 1398 from areas close to the coast of Bushehr province. Bacteria that cause Vibriosis were determined using biochemical tests and molecular sequencing. Bacterial selection was performed according to the results of antibiogram profiles of dominant bacteria and pathogens. TCBS was used to determine the phage titer of the culture medium and the plugs were counted 24 hours after incubation at 30 ° C.5 bacteria (Vibrio alginolyticus strain SeqID, Vibrio xuii strain Z-03Phage, Vibrio alginolyticus SZ / 10 gene, Vibrio rotiferianus strain SRPR-Phage, Vibrio harveyi strain Z05 were isolated from Bushehr wastewater contaminated waters). Vibrio alginolyticus strain SeqID was selected as the dominant bacterial species according to the results of antibiogram profile determination, growth halo diameter and bacterial biochemical test of the studied species. Based on the results of 16srDNA molecular analysis, the isolated strain of the bacterium was 100% similar to Vibrio alginolyticus. Based on the results of the host domain, Vibrio alginolyticus strain Samira Phage was able to form plugs.The isolated bacteriophage Vibrio alginolyticus strain Samira Phage had a bactericidal effect with a wide range against Vibrio bacteria identified from the shores of Bushehr, which indicates that this bacteriophage can be a good candidate for use in phage therapy Be against viberosis.Keywords: Isolation and screening, Bacteriophage, Vibriosis, Western white shrimp
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