List of Articles Amniotic Fluid

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Evaluation of the Effects of Methamphetamine on Amniotic Sac Composition in Pregnant Mice and Their Offspring
  Milad Rezazadeh Ramin Hajikhani Gholamhassan Vaezi Maryam Bananej
  10.22034/ascij.2022.1934666.1266
  Methamphetamine is a central nervous system stimulant drug that has become very popular among adolescents and young people in recent years. Since the use of drugs including methamphetamine can have a potential effect on amniotic fluid, expression of genes can affect mot More

  Methamphetamine is a central nervous system stimulant drug that has become very popular among adolescents and young people in recent years. Since the use of drugs including methamphetamine can have a potential effect on amniotic fluid, expression of genes can affect mother and fetus, tissues, hormones, and other factors, this study was aimed at investigating the effects of methamphetamine on the amniotic sac of pregnant rat and its effects on subsequent generations. In this study, 60 adult Wistar female rats were assigned to six groups: control group, sham group, group receiving 1 mg/kg amphetamine, group receiving 2 mg/kg amphetamine, group receiving 3 mg/kg amphetamine, and group receiving 4 mg/kg amphetamine. At the end of the experimental period, blood samples were taken from the hearts of mice and the desired parameters (testosterone, glucose, LH, FSH, AST, ALT, WBC, and RBC) were measured. The rats were then dissected; amniotic fluid was isolated to evaluate the amount of protein, carbohydrates, fats, phospholipids, and urea. Intraperitoneal injection of methamphetamine caused significant changes in the amount of biochemical parameters measured such as LH, T.G, and testosterone compared to the control group. In addition, methamphetamine did not alter FSH. With increasing methamphetamine dose, expression of HOX and TUBB8 genes decreased. The results of this study show that even at low doses, methamphetamine injection increases or decreases ovarian activity and ovogenesis, resulting in impaired ovulation quality and oocyte maturation. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - Effect of Heroein on Amniotic Sac Compounds in Pregnant Rats and Ovarian of Their Female Offspring
  Fatemeh Osanloo Ramin Haji khani Gholam hassan Vaezi Maryam Bananaj
  10.22034/ascij.2022.1940732.1310
  Heroin is one of the most addictive drugs used among adolescents and young people in Iran. Since the use of drugs such as Heroin has detrimental effects on organs, tissues, hormones, amniotic fluid, and expression of genes affecting the mother and fetus, as well as othe More

  Heroin is one of the most addictive drugs used among adolescents and young people in Iran. Since the use of drugs such as Heroin has detrimental effects on organs, tissues, hormones, amniotic fluid, and expression of genes affecting the mother and fetus, as well as other factors, the aim of this study was to investigate the effects of methamphetamine on the amniotic sac of pregnant rat and its effects on subsequent generations. Sixty adult Wistar female rats were assigned to six groups: control group, sham group, group receiving 1 mg/kg heroin, group receiving 2 mg/kg heroin, group receiving 3 mg/kg heroin and the group receiving 4 mg/kg heroin. At the end of the experimental period, blood samples were taken from the hearts of the mice and the desired parameters such as testosterone, glucose, LH, FSH, AST, ALT, WBC, and RBC were measured. The rats were then dissected; amniotic fluid was isolated to assess the amount of carbohydrates, protein, phospholipids, fats, and urea. Finally, statistical analysis was performed. Intraperitoneal injection of heroin caused significant changes in testosterone and T.G parameters compared to the control group. However, no significant change was observed in LH and FSH levels. In addition, many changes such as weakening and thinning of the theca layer in the graph follicle, destruction of the underlying material and growing follicles following heroin use in the ovarian tissue. Moreover, increased gene expression was seen after increasing the dose of heroin. In this study, heroin injection, even in low doses, could increase or decrease ovarian activity and oogenesis, resulting in impaired oocyte maturation and ovulation quality. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  3 - The comparison of effect of spectral doppler with 2 dimensional sonography on frequency of fetal movements in the late of first trimester
  Amir Hossien Hashemi Attar Maryam Sadat Shakeri
  Background: Many anatomical disorders and fetal congenital abnormalities can be diagnosed using sonography, such as screening the first three months for Down syndrome (Nuchal Translucency (NT) measurement). The special fetus position is necessary for NT measurement, and More

  Background: Many anatomical disorders and fetal congenital abnormalities can be diagnosed using sonography, such as screening the first three months for Down syndrome (Nuchal Translucency (NT) measurement). The special fetus position is necessary for NT measurement, and the presence of the fetal movements accelerates the measuring process. The effect of the external factors on increasing the fetal movements in the last three months of pregnancy has already been investigated. However, there is not much information about the stimulating factors for the first three months of pregnancy. Therefore, this study investigates the effect of Doppler sonography on the fetal movements by the end of the first three months of pregnancy. Materials and Methods: In this study, 210 fetuses with gestational age of 11 to 14 weeks (based on Crown-rump length (CRL)) were examined. Cases such as multiple birth, pregnancy with smoking and drug usage history, mothers with more than 4 pregnancies, and abnormal level of Amniotic Fluid volume were excluded.  Three types of fetal movement (isolated arm, isolated foot and jumping) were counted by one sonologist using one sonography system. Fetuses were divided into two groups each examined for two minutes. The first group was subjected to 2D sonography waves, and the second group to one-minute 2D sonography, 10-second Doppler waves, and another 50-second 2D sonography in succession. In each group, fetal movements were counted continuously. Recorded data has been analyzed using SPSS software. Results:  The sameness of gestational age, CRL and NT in two groups were proved by statistical tests. The variation of the hand movements in the first group (mean=0/1, standard deviation = 2/67) and the second group (mean=1/4, standard deviation = 2/91) were compared, and the statistical significance has been found (statistic=3/12, p-value=0/002) which shows the increase in the hand movements due to the Doppler ultrasound.  Moreover, the variation of the foot movements in the first group (mean=0/08, standard deviation = 2/34) and the second group (mean=1/19, standard deviation = 2/71) had statistical significance (statistic=3/21, p-value=0/001). Additionally, the variation of the jumping movements in the first group (mean=0/22, standard deviation = 2/29) and the second group (mean=0/81, standard deviation = 2/75) had also the statistical significance with statistic of 2/41 and p-value of 0/016. Conclusion: It is observed that the Doppler ultrasound waves increased the fetal movements while the 2D ultrasound waves were ineffective.   Manuscript profile