List of Articles کلرید آهن

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - The effect of homologous and heterologous Bacterial DNA on the development of immune responses against Pasteurella multocida in BALB / c mice
  Yahya Tahamtan Maryam Homayoon Mohammad Kargar
  Background & Objectives: Pasteurella multocida is a principal pathogen of domestic animals causing agents of pneumonia and hemorrhagic septicemia in cattle, sheep, and goats, fowl cholera in chickens, and progressive atrophic rhinitis in swine and as well as an oppo More

  Background & Objectives: Pasteurella multocida is a principal pathogen of domestic animals causing agents of pneumonia and hemorrhagic septicemia in cattle, sheep, and goats, fowl cholera in chickens, and progressive atrophic rhinitis in swine and as well as an opportunistic pathogen of humans. In this study, we investigated the humoral and cellular immune responses and protective immunity conferred by an iron-inactivated vaccine with two different bacterial DNA as an adjuvant. Materials & Methods: P. multocida was grown in BHI broth, inactivated with FeCl3, adjuvanted with alum and P. multocida A (homologous DNA) and P. multocida B (heterologous DNA) bacterial DNAs. BALB/c mice were immunized with two whole-cell inactivated vaccine doses at 2 weeks apart. The animals were challenged 4 weeks after booster immunization. The serum antibodies titer was tested by ELISA. At 28 days post-immunization, cell mediates immunity responses were measured by assay of DTH and IL-6 and IL-12 in the serum samples. Results: Our results showed the levels of antibodies in bDNA adjuvant groups were higher than the alum adjuvant vaccine group. Peak antibody titers of 0.372 were obtained in the IIA+AbDNA group. The protection rate of the AbDNA adjuvant vaccine was better than of other adjuvant vaccines and they protected 100% of mice challenge groups. Peak serum IL-6 and IL-12 titers were achieved in the IIA+AbDNA groups. Conclusion: These studies indicate that bDNA is effective as immune adjuvants and because of its stimulating properties it can be used as an inducer of humoral and cellular immune responses for vaccination applications. The findings also showed a better ability of homologous bDNA to induce immune responses. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - The determination and measurement of some secondary metabolites of leaves, stems and roots of Dendrostellera lessertii(Wikstr)Van. Tigeh. and allelopathy effects on barley and mungbean plants
  B. Delnavaz O. Ataei M. Mojdehi
  Dendrostellera lessertii )Thymelaeaceae family) is one of the herb and medicinal plants with anti-cancer agents. In this study, active substance of roots, stems and leaves of D. lessertii was determined and assayed. Allelopathic effects of some concentration of alcoholi More

  Dendrostellera lessertii )Thymelaeaceae family) is one of the herb and medicinal plants with anti-cancer agents. In this study, active substance of roots, stems and leaves of D. lessertii was determined and assayed. Allelopathic effects of some concentration of alcoholic extract of D. lessertii were evaluated on barley (Hordeum vulgare L.) and mungbean(Vigna radiata L.). The treatments were include organs (leaves, root and stem), concentrations (0, 50 anf 100 mgml-1) and seeds (barley and mungbean). All experiments occurred in laboratories of Saveh University and in completely random blocks design.The present of effective compounds include dense tannins; hydrolyzed tannins and alkaloids identified using TLC analysis and the color reaction with Dragendorff and Ferro chloride reagents. The results showed all organs of D. lessertii are rich of phenols, tannins and alkaloids but in roots and leaves had the highest contents. Phenolic compounds of D. lessertii were very high and hydrolyzed tannins were more than dense tannins. To compare with other plants, alkaloids of D. lessertii organs were the high but phenols and hydrolyzed tannins were low. The results showed D. lessertii alcoholic extract reduce seedlings growth and 66% and 33% seeds germination of barely and mungbean sequentially. Alcoholic extract have allelopathic effects on seeds germination and seedlings growth of barley and mungbean but on barley was more inhibited. Manuscript profile