List of Articles کشت درون شیشه‌ای

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  1 - The Effect of Plant Growth Regulators and Different Explants on the Response of Tissue Culture and Cell Suspension Cultures of German Chamomile (Matricaria chamomilla L.)
  L. Koohi, N. Zare R. Asghari-Zakaria P. SheikhZadeh-Mosaddegh
  German chamomile (Matricaria chamomilla L.) is one of the most important medicinal plants that its essential oils used in different medicinal industries. In this study which was carried out in 2013 growing season at the Faculty of Agricultural Sciences of the University More

  German chamomile (Matricaria chamomilla L.) is one of the most important medicinal plants that its essential oils used in different medicinal industries. In this study which was carried out in 2013 growing season at the Faculty of Agricultural Sciences of the University of Mohaghegh Ardabili, the in vitro response of leaf and hypocotyl explants of German Chamomile in B5 medium supplemented with different levels of plant growth regulators including 2,4-D, naphthalene acetic acid (NAA), kinetin and 6-benzylaminopurine (BAP) were investigated in a factorial experiment based on completely randomized design (CRD).In addition, cell suspension cultures were established and characterized. Hypocotyl and leaf explants exhibited cell proliferation and produced callus within 1-2 weeks. The highest fresh weight of the callus (264.1 mg) was produced by leaf explants in the medium supplemented with 0.5 mg/l 2,4-D and 1 mg/l BAP. However, the leaf explants cultured on medium containing 1.5 mg/l 2,4-D showed the lowest cell proliferation and callus yield (40.42 mg). The highest percentage of root induction from leaf explants (58.73%) was observed on the medium containing 4 mg/l 2,4-D and 1 mg/l Kin, and from hypocotyl explants (48.61%) was observed on medium supplemented with 1.5 mg/l NAA. The 42.22% of calli derived from hypocotyl explants on B5 medium supplemented with 4 mg/l NAA and 3 mg/l BAP, were friable. Cell suspension cultures of German chamomile were established by transferring of hypocotyl-derived friable calli into the MS medium supplemented with 1.5 mg/l 2,4-D and 1 mg/l kinetin. The growth curve of cell proliferations started 4 days after culture and continued to grow until day 13th, where the cells entered stationary phase. Manuscript profile
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  2 - تاثیر تنش خشکی و پاکلوبوترازول بر میزان تجمع فلاونوئیدها و عناصر معدنی در گیاه کلزا (L. Brassica napus) در شرایط کشت درون شیشه
  مهری عموبیگی رویا رضوی زاده
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  3 - Study of effects of different plant growth regulators on explant's establishment of Aloe vera (Aloe barbadensis Mill.)
  A.B Barzgar مرضیه Shafiee HajiAbad محمود Abdy
  Aloe (Aloe barbadensis Mill) is one of the most important medicinal plants belonging to Liliaceae family that nowadays is propagated by in vitro culture in high-scale propagation. Because of the impotance of the establishment stage in in vito culture of this plant, this More

  Aloe (Aloe barbadensis Mill) is one of the most important medicinal plants belonging to Liliaceae family that nowadays is propagated by in vitro culture in high-scale propagation. Because of the impotance of the establishment stage in in vito culture of this plant, this study was conducted to investigate the best media culture for Aloe explants establishment. First, the prolifration rate of two kind of explants (Shoot tips and auxiliary buds) were evaluated on 8 media cultures and shoot tips selected as the best explants. Then, in order to determine the best media culture an experiment was conducted in factorail design with two factors: auxins in four levels (1 mgl-1 IAA, 0.5 mgl-1IAA, 1 mgl-1IBA, 0.5 mgl-1IBA) and cytokinins in two levels (BA and Kinetin at 1 mgl-1) in a complete randomised design with 3 replications. After 35 days the highest rate of prolifration earned on the media contained 0.5 mgl-1 IAA and 1 mgl-1 BA with 6.0 shoots per explant and 2.3 cm length, that is higher and faster than other related reports. Since on the stablishment stage of in vitro culture regnaration of the highest shoot number per explant with small size has considerable attention, this protocol suggestes an appropriate method for establishment of Aloe explants. Manuscript profile
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  4 - یک روش ساده و سریع برای ریزازدیادی گل اطلسی رقم مرلین مورن صورتی (Petunia × hybrida F1 )
  شهرام مهری محمد نبی ایلکایی فرزین سعیدزاده
  برای تکثیر مطلوب گل اطلسی رقم ’مرلین مورن صورتی‘، ما یک روش ساده ریزازدیادی آزمایشگاهی ابداع کردیم. برای این منظور جوانه‌های انتهایی 30 روزه حاصل از گیاهان رشد یافته در محیط آزمایشگاهی از بذرهای هیبرید، در محیط موراشیگ و اسکوگ (MS) تیمار شده با 36 ترکیب تیما More

  برای تکثیر مطلوب گل اطلسی رقم ’مرلین مورن صورتی‘، ما یک روش ساده ریزازدیادی آزمایشگاهی ابداع کردیم. برای این منظور جوانه‌های انتهایی 30 روزه حاصل از گیاهان رشد یافته در محیط آزمایشگاهی از بذرهای هیبرید، در محیط موراشیگ و اسکوگ (MS) تیمار شده با 36 ترکیب تیماری حاصل از سطوح 6- بنزیل‌آمینوپورین (0، 5/0، 1، 2، 3 و 5 میلی گرم BA در لیتر) در سطوح آلفا نفتالین استیک اسید (0، 1/0، 5/0، 1، 5/1 و 3 میلی گرم NAA در لیتر) کشت داده شدند. نتایج نشان داد که فقط BA در مقدار 5/0 یا 2 میلی‌گرم در لیتر برای طول ساقه، تعداد ساقه، تعداد گره و تعداد برگ در مقایسه با سایر غلظت‌های BA و BA در ترکیب با NAA، بهتر بود. بیشترین تعداد شاخه فرعی (44/8) و حداکثر طول ساقه (16/13سانتیمتر) در محیط MS حاوی 50/0 میلی‌گرم در لیتر BA بدون NAA ثبت شد. حداکثر طول ریشه (2/5 سانتی متر) و تعداد ریشه (77/8) در محیط حاوی 10/0 میلی‌گرم NAA لیتر مشاهده شد. گیاهان پرورش یافته در محیط آزمایشگاهی در داخل گلدان‌های حاوی پرلیت و کوکوپیت کاشته شدند و در طی 4 هفته با بقای 100٪ رشد کردند. گیاهان تکثیر شده از لحاظ مورفولوژیکی با گیاهان مادری مشابه بودند و هیچ گونه تنوع فنوتیپی قابل تشخیص مشاهده نشد. Manuscript profile
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  5 - Micro propagation of (Rosa hybrida L. cv. Maurossia) and Comparative anatomy of vegetative organs in vivo and in vitro plants
  parissa jonoubi
  Abstract. Rosa hybrid L. is one of the most important ornamental plants that one of the modern methods of micropropagation of Rosa hybrid Cv.Maurossia is tissue culture. In this research, after optimizing of sterilization of explants with sodium hypochlorite, MS culture More

  Abstract. Rosa hybrid L. is one of the most important ornamental plants that one of the modern methods of micropropagation of Rosa hybrid Cv.Maurossia is tissue culture. In this research, after optimizing of sterilization of explants with sodium hypochlorite, MS culture medium with various concentrations of BA was used for the establishment. The highest growth rate was observed in 0.2 mg l-1 BA. Then, explants were transferred to MS medium with different concentrations of BA, NAA and IAA. The results showed the optimal branch regenerated was obtained at 2 mg l-1 BA and 0.5 mg l-1 IAA. In indirect organogenesis, the part of the leaf with the petioles was used and the optimum percentage was observed in 2 mg l-1 BA with 1 mgl-1 NAA. Then, explants transferred to the rooting induction medium of MS and MS/2 with IBA and Phloroglucinol. The most rooting induction was observed in MS/2 medium with 1 mg l-1 BA and 0.2 mg l-1 Phl (Phloroglusinol). Also, due to the importance of the morphology of the Rosacea family, the anatomical studies of the vegetative organs were compared in two samples of in vivo and in vitro plants and the results showed that the general structures in both samples are similar. Manuscript profile
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  6 - Optical differences of ex vitro and in vitro plantlets of Boston fern, Nephrolepis exaltata Schott cv. Bostoniensis
  Marzieh Shafiee Hajiabad reza Fotohi Ghazvini
  In vitro formed plantlets perceive environmental stresses in transferring to in vivo condition due to their special morphological characteristics. Precise determination of differences between in vitro and acclimated plants can lead to successful acclimation of plantlets More

  In vitro formed plantlets perceive environmental stresses in transferring to in vivo condition due to their special morphological characteristics. Precise determination of differences between in vitro and acclimated plants can lead to successful acclimation of plantlets. Recently, optical studies are exploited as nondestructive and fast methods in exact determination of these differences. The current study was conducted to determine anatomical and morphological differences between ex vitro and in vivo plants by optical method. In this study, in vitro and acclimated plantlets of Boston fern, Nephrolepis exaltata Schott cv. Bostoniensis, which were cultured and rooted on 1/2 MS basal medium supplemented with 20 g.l-1 sucrose and 0.5 mg.l-1 benzyl adenine (BA) were used. Tri-dimensional pictures revealed that the surface of acclimated plant’s leaves were about 6-10 times smoother than in vitro ones. The comparison of reflective spectrum of in vitro and acclimated plantlets showed the most differences in the amplitude of the green wavelength (545-555 nm) and the red wavelength (670-685 nm). Manuscript profile