List of Articles موش آزمایشگاهی

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  1 - Effect of folic acid in cholestatic hepatic fibrosis induced by bile duct ligation Model in rat
  Z. Mohammadian, A. Eidi, P. Mortazavid, SM. Tavangar, A. Asghari,
  Cholestasis is a liver disease that, if untreated and not prevented, will cause hepatic fibrosis andcirrhosis, and eventually death. As a result of bile duct ligation (BDL), toxic bile acidsaccumulate in liver. Accumulation of these toxins and subsequent events, such as More

  Cholestasis is a liver disease that, if untreated and not prevented, will cause hepatic fibrosis andcirrhosis, and eventually death. As a result of bile duct ligation (BDL), toxic bile acidsaccumulate in liver. Accumulation of these toxins and subsequent events, such as oxidativestress and inflammatory response leads to cell death and hepatic fibrosis. The liver is the mainorgan for folate storage and metabolism and folate deficiency is a common occurrence in manyliver diseases. The present study investigated the protective effect of folic acid in experimentalhepatic fibrosis. 81 male Wistar rats were classified into the nine groups؛ Control, Shamoperatedcontrol, Folic acid, Bile duct-ligated (BDL) control, and BDL+ folic acid groups. Folicacid treated groups were given oral folic acid (1, 5 and 10 mg/kg b.w) for 28 days. The severityof hepatic injury was determined by measures biochemical indicators like’s activities of AST,ALT, ALP, concentrations of bilirubin and albumin in the serum and activities of superoxidedismutase in the hepatic samples. To assess histopathological features of cholestasis (bile ducthyperplasia, fibrosis, necrosis and inflammatory cell infiltration), Masson’s trichrome stainingwas performed. In BDL rats treated with folic acid, hepatic fibrosis was significantlyameliorated and the serum and hepatic biochemical variation induced by BDL were moderated.This study suggests that folic acid has a protective effect in liver. Folic acid reduced oxidativestress and inflammatory response and thus prevented liver fibrotic changes. Manuscript profile
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  2 - Effect Fenugreek seeds hydroethanolic extract on a full-thickness wound healing in Streptozotocin-induced diabetic mice
  Farahpour, M.R., Aghaei, M. .
  Disturbance in wound healing process is considered to be one of the most serious complications of diabetes, because it significantly increases the susceptibility of patient wounds to be infected. Fenugreek is an annual plant which its leaves and seeds used extensively i More

  Disturbance in wound healing process is considered to be one of the most serious complications of diabetes, because it significantly increases the susceptibility of patient wounds to be infected. Fenugreek is an annual plant which its leaves and seeds used extensively in foods and has advantages to human health. Due to prevalence rate and side effects of diabetes, Fenugreek seed extract is one of medicinal herb have been used more frequently. The present study was performed to investigate the effects of using fenugreek seed hydroethanolic extract on wound healing process in the 54 mice model of diabetes type 2. One single full-thickness excisional wound was created with 6-mm punch biopsy on back of each mice. All animal, accidentally, divided in three groups: Control group, 10% and 5% fenugreek seed hydroethanolic extract treatment groups. In terms of microscopic, the rate of tissue edema, neo-vascularization, fibroblast infiltration, collagen deposition and re- epithelialization were evaluated. Results revealed that the edema score significantly decreased (P<0.05) in both treatment groups compared to the control group, whereas the neo-vascularization, fibroblast infiltration, collagen deposition and re- epithelialization significantly increased (P<0.05) in both treatment groups compared to the control group. These results showed that topical administration of fenugreek seed hydroethanolic extract promote skin full-thickness excision wound healing process in diabetic animal model.  Manuscript profile
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  3 - Study of behavioral and Physiological effects due to contamination with Argas persicus larvae in mice
  maryam karimi dehkordi , F Rezaei , Sh Poornazari
  Argus persicus is found in tropical and subtropical regions of the world. Poultry, pigeons, ducks, geese, and other domestic and wild birds and even humans are its hosts. In addition to causing anemia, the mite can also transmit avian Spirochetosis and Pasteurellosis. T More

  Argus persicus is found in tropical and subtropical regions of the world. Poultry, pigeons, ducks, geese, and other domestic and wild birds and even humans are its hosts. In addition to causing anemia, the mite can also transmit avian Spirochetosis and Pasteurellosis. The aim of this study was to investigate the physiological and behavioral effects of feeding Argas persicus larvae on rats. After collecting Argas persicus larvae, rats were infected with the desired number of Argas persicus tick larvae in different treatments, and physiological symptoms were examined and recorded daily. The results of the present study indicated abnormalities and physiological changes in rats infected with the larvae of this tick. Early skin signs also indicated a slight inflammation of the skin at the junction of the infants and varied according to the number of ticks used to infect the rats. Also, weight changes in infection with different doses of larvae indicated the effect of the tick on weight. Considering the negative effects of this tick and the economic losses caused by this tick, further investigation of the effects of this tick in poultry seems necessary.  Manuscript profile
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  4 - Study of behavioral and Physiological effects due to contamination with Argas persicus larvae in mice
  maryam karimi dehkordi , F Rezaei , Sh Poornazari
  Argus persicus is found in tropical and subtropical regions of the world. Poultry, pigeons, ducks, geese, and other domestic and wild birds and even humans are its hosts. In addition to causing anemia, the mite can also transmit avian Spirochetosis and Pasteurellosis. T More

  Argus persicus is found in tropical and subtropical regions of the world. Poultry, pigeons, ducks, geese, and other domestic and wild birds and even humans are its hosts. In addition to causing anemia, the mite can also transmit avian Spirochetosis and Pasteurellosis. The aim of this study was to investigate the physiological and behavioral effects of feeding Argas persicus larvae on rats. After collecting Argas persicus larvae, rats were infected with the desired number of Argas persicus tick larvae in different treatments, and physiological symptoms were examined and recorded daily. The results of the present study indicated abnormalities and physiological changes in rats infected with the larvae of this tick. Early skin signs also indicated a slight inflammation of the skin at the junction of the infants and varied according to the number of ticks used to infect the rats. Also, weight changes in infection with different doses of larvae indicated the effect of the tick on weight. Considering the negative effects of this tick and the economic losses caused by this tick, further investigation of the effects of this tick in poultry seems necessary. Manuscript profile
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  5 - بررسی آلودگی موش‌های آزمایشگاهی یک مرکز پرورش حیوانات آزمایشگاهی به ویروس تیلر عامل انسفالومیلیت
  روزبه فلاحی فاطمه عابدینی همایون مهروانی
  10.30495/jvm.2020.676178
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  6 - Physiological and behavioral effects of feeding of Argas reflexus larvae in laboratory mice
  Farid Rezaei Maryam Maryam Karimi-Dehkord Forogh Khavaran
  Inroduction & Objective: Argas reflexus or pigeon tick is a soft tick of Argas genus. This tick is an external parasite of poultry that often feeds on pigeon blood and is one of the most important pigeon ectoparasites that can endanger human health. The aim of this More

  Inroduction & Objective: Argas reflexus or pigeon tick is a soft tick of Argas genus. This tick is an external parasite of poultry that often feeds on pigeon blood and is one of the most important pigeon ectoparasites that can endanger human health. The aim of this study was to investigate the physiological and behavioral effects of feeding Argas reflexus larvae on mice. Material and Method: After collecting the Argas reflexus ticks and in vitro production of their larvae, mice were infected with 30, 40 and 50 larvae in three treatments, respectively, and 5 mice were treated in each treatment. Then, Physiological symptoms such as changes in weight and temperature, behavioral changes and skin symptoms were monitored and recorded daily. Results: The results of this study show with increasing the number of larvae in infected mice, the severity of symptoms also increase. It also seems that in addition to the number of larvae, the longer feeding of larvae from the body of laboratory animals, the greater the severity of clinical and cutaneous symptoms and their stability. Conclusion: According to the results, it is necessary to pay attention to the dangers of the presence of this tick in birds as the main host of this tick and also in the habitat of humans and to develop control and preventive programs against this tick. Manuscript profile
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  7 - بررسی اثر عصاره آبی برگ توت سفید (.Morus alba L) بر میزان قند و چربی های خون در موش های صحرایی نر
  رحمت اله پرندین رضا خدارحمی شفیع امینی مقدم
  مقدمه و هدف: دیابت شیرین یکی از مهم ترین مشکلات پزشکی در اغلب کشورها و به ویژه ایران می باشد. این بیماری در واقع ناشی از اختلال در سوخت و ساز قندها، چربی ها و پروتئین های بدن می باشد. هدف از انجام این پژوهش ارزیابی اثر درمانی برگ های گیاه توت سفید (L. Morus alba) بر موش More

  مقدمه و هدف: دیابت شیرین یکی از مهم ترین مشکلات پزشکی در اغلب کشورها و به ویژه ایران می باشد. این بیماری در واقع ناشی از اختلال در سوخت و ساز قندها، چربی ها و پروتئین های بدن می باشد. هدف از انجام این پژوهش ارزیابی اثر درمانی برگ های گیاه توت سفید (L. Morus alba) بر موش های آزمایشگاهی دیابتی شده بود.روش تحقیق: در این مطالعه تجربی تعداد 30 موش بالغ نر نژاد ویستار با وزن بین 200 تا 250 گرم به طور تصادفی در 5 گروه (دو گروه موش سالم و سه گروه دیابتی) تقسیم شدند. در ابتدا سه گروه تجربی با استفاده از تزریق داخل صفاتی mg/kg 60 استرپتوزوتوسین دیابتی شده و به یک گروه از موش های دیابتی روزانه mg/kg600 عصاره آبی توت سفید برای مدت 35 روز خورانده شد. به یک گروه از موش های دیابتی نیز بدون انجام درمان خاص در نظر گرفته شد و گروه دیگر موش های دیابتی نیز روزانه انسولین NPH تزریق شد. یک گروه از موش های سالم روزانه  mg/kg600 عصاره آبی توت سفید و گروه دیگر موش های سالم نیز طی همین مدت فقط سرم فیزیولوژیکی دریافت می کردند. در پایان میزان قند و چربی های خون با روش های متداول اندازه گیری شد.نتایج و بحث: در این تحقیق مشخص گردید که عصاره آبی برگ توت سفید سبب کاهش معنی دار غلظت گلوکز (001/0< P)، کلسترول (05/0< P)، تری گلیسرید (01/0< P) و LDL (05/0< P) و افزایش معنی دار میزان HDL (05/0< P) سرم درموش های دیابتی در مقایسه با موش های دیابتی بدون درمان خاص شده است.توصیه کاربردی/صنعتی: نتایج این تحقیق نشان داد که عصاره آبی برگ توت سفید میزان قند و چربی های خون را در موش های سالم تغییر نمی دهد، ولی در موش های دیابتی سبب کاهش مقدار قند و چربی های خون می­شود و  در صورت کسب نتایج بالینی مطلوب و با توجه به اینکه برگ این گیاه به وفور یافت می شود ممکن است کمک شایانی به تولید صنعتی داروی کاهش قند خون نماید. Manuscript profile
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  8 - The Effects of Inhaling the Toxic Mixture of Bleach and Detergent Materials on Leukocytes of Mice
  غلامحسن واعظی فاطمه طوسی عبدالحسین شیروی مهسا پورکاظم فرشته علی آبادی
  Today,bleachandcleaningmaterialsarethemost commonproductsthatareused bythe public.Sometimes amixtureofbleachanddetergentsforcleaninguse someoftheChemistryof thefurtherrelease ofchlorine gaspoisoningandthesymptomsaresevere.Chlorinegashas been knownas a mucus membranes an More

  Today,bleachandcleaningmaterialsarethemost commonproductsthatareused bythe public.Sometimes amixtureofbleachanddetergentsforcleaninguse someoftheChemistryof thefurtherrelease ofchlorine gaspoisoningandthesymptomsaresevere.Chlorinegashas been knownas a mucus membranes and respiratory tract irritant .This gas can increase free radicals. Free radicalscancausecell damage.Theeffects ofinhalingthe gasanditsconsumptiononbody tissuesandbloodcells,complete informationis available The aimof this studythe effects ofinhalingtoxicmaterials,bleachanddetergentmixtureofbloodcellsinthemice.42adult male mice NMRIweighing35andplusmn;3 g and10 to 8weeksto6 groupswere divided intoexperimentalandcontrolgroups. Experimentalgroup1 - 2 -3 withthechamberaglass(chamber)to beinhaledfor 20 minutestosprayamixtureof1 ccof bleachanddetergent were usedby thenebulizer.Experimentalgroups4-5-6, 35minutestoinhalethe same amountofmaterialreceivedmiceat24 to 48-72 hoursafterinhalationofblood sampling, they werethenGiemsastainedslidespreparedandexaminedNewbury.Thestudywasdoneby increasing theexposuretimeincreasedsignificantly thenumber ofleukocytesanderythrocyteswere foundtohavebeentransformed.Increasedduration ofexposure toa mixture ofbleachanddetergent, and over time changesincreased thenumber ofleukocytes. Manuscript profile
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  9 - A Comparative Analysis of the Effects of Garlic, Elderberry, and Black Seed Extract on the Leukocyte in Mice
  مهرداد مدرسی
  The herbs Garlic, Elderberry, Black Seed have acknowledged therapeutic use in traditional medicine. With their immune-effective compounds, they are of much application in the traditional treatment of infections; the present study attempts a comparative investigation of More

  The herbs Garlic, Elderberry, Black Seed have acknowledged therapeutic use in traditional medicine. With their immune-effective compounds, they are of much application in the traditional treatment of infections; the present study attempts a comparative investigation of the roles of the aforementioned herbs in the immune system and blood proteins. In this paper, the mice of the Balb/C genus were divided into four groups: control, 1, 2, and 3. Each of these received 50, 100, 200 mg/kg of garlic, elderberry, and Black Seed extracts respectively. The extract was injected using the inner peritoneum (IP) method every other day for 20 days. At the end of the experiments, blood samples were taken to conduct a count of white blood cells and an Electrophoresis of blood proteins. The results were evaluated through SPSS (Duncan test) at a Pandlt;0.05 level. The analysis indicates that Black Seed and garlic extract were able to increase the number of white blood cells. While Black Seed gave rise to the amount of white blood cells and also caused the Albumin, alpha-1 and alpha-2 proteins and the A/G ratio to increase, garlic was effective in raising alpha-1 and gamma globulin levels. This is in contrast with elderberry, which plays a significant role in decreasing the gamma proteinandrsquo;s density.The two herbs, garlic and Black Seed, impressed the immune system through changing the density of such blood proteins Albumin, alpha-1, alpha-2 and altering the number of white blood cells; whereas the result suggest no significant effect in the case of elderberry. Manuscript profile
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  10 - The Neuroprotective Effect of Xanthone and 6-Hydroxyflavone in the Model of Parkinson's Disease Induced by 6-Hydroxydopamine in Laboratory Mice: Behavioral Evaluations
  Mahshid Attari Maryam Khosravi Ramin Hajikhani Maryam Bananaj Jalal Solati
  10.22034/ascij.2022.1928825.1252
  Oxidative stress and neuroinflammation play a role in Parkinson's disease. Antioxidants and anti-inflammatories such as polyphenol compounds and flavonoids inhibit neuronal death. The aim of the present study is the effect of xanthone and 6-hydroxyflavone in Parkinson's More

  Oxidative stress and neuroinflammation play a role in Parkinson's disease. Antioxidants and anti-inflammatories such as polyphenol compounds and flavonoids inhibit neuronal death. The aim of the present study is the effect of xanthone and 6-hydroxyflavone in Parkinson's disease in laboratory mice. Animals are cannulated by stereotaxic surgery and unilateral injection of 6-hydroxy-dopamine is performed in the dense area of ​​the substantia nigra (SNc) of the brain. Xanthone and 6-hydroxyflavone were injected intraperitoneally. Three weeks after surgery, movement evaluations and pseudo-anxiety and pseudo-depression behaviors were performed. Counting of all the neurons in the dense area of ​​the substantia nigra was done. Injection of 6-hydroxydopamine increased the number of apomorphine rotations. Catalysis time increased. Neurons in the substantia nigra decreased. 6-Hydroxyflavone (50 and 100 mg/kg) and xanthone (100 and 200 mg/kg) reduced vertigo and catalepsy. In the elevated plus shape maze test, 6-hydroxyflavone in doses of 25 and 50 mg/kg and xanthone in doses of 50 and 100 mg/kg increased motor activity. In the forced swimming test, xanthone in doses of 50, 100 and 200 mg/kg reduced immobility in parkinsonian rats. The number of substantia nigra neurons increased with the treatment of 6-hydroxyflavone in doses of 50 and 100 mg/kg and 200 mg/kg xanthone. Xanthone and 6-hydroxyflavone improved movement disorder and catalepsy and increased the number of nerve cells in the substantia nigra. Xanthon was able to reduce depression. Probably, part of these central protective effects are mediated by the antioxidant and anti-inflammatory effects of xanthone and 6-hydroxyflavone, which prevent cell death by reducing free radicals and inflammatory cytokines, and as a result, they improve cognitive and movement disorders. Manuscript profile