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      • Open Access Article

        1 - Effect of copper nitrate on some physiological and biochemical indices in ship sturgeon (Acipenser nudiventris)
        A. Mohamadrezayei S. Abdali A. Yousefi Jourdehi
        In order to evaluate copper nitrate (Cu (NO3)2) effects on some blood biochemical indices in Acipenser nudiventris, 120 fish were exposed to 0, 1, 5 and 10 mg/L of Cu (NO3)2  in 12 aquariums with  a volume 100 liters. Blood samples were collected from fish at More
        In order to evaluate copper nitrate (Cu (NO3)2) effects on some blood biochemical indices in Acipenser nudiventris, 120 fish were exposed to 0, 1, 5 and 10 mg/L of Cu (NO3)2  in 12 aquariums with  a volume 100 liters. Blood samples were collected from fish at 24, 48, 72 and 96 hours. The highest percentage of mortality of up to 24 hours after exposure to the toxin was related to concentrations of 10 (70 percent) and 5 (50%) and 1 (10%) mg/l, respectively. After 24 hours, fish died at 5 and 10 mg/l of copper. However, at concentration of 1 mg/L up to 96 hours almost 20 percent of fish were resistant. No mortality was observed in the control group. Triglyceride levels at concentrations of 10 mg/l Cu (NO3)2, was 479 ± 7.7 mg/dl after 24 h and decreased significantly in comparison with the control (709.3 ± 69.3 mg/dl) and at 1 mg/l concentration of Cu (NO3)2 in 48, 48, 72 and 96 hours it reached 237.9 ± 4.2, 23.5 ± 0.8 and 226 ± 0.6 mg/d, respectively (P < 0.05). Cholesterol level at concentrations of 5 and 10 mg/l Cu(NO3)2, was 206.6 ± 7.7 and 229.3 ± 0.6 mg/dl, respectively, after 24 hrs, which had increased significantly compared to the control and 1 mg/l Cu(NO3) concentration (P < 0.05). Glucose levels, at 1 mg/l concentration of Cu (NO3)2 , were 81.5 ± 1.4, 73 ± 1 and 69.3 ± 0.6 mg/dl in 48, 72 and 96 hours, respectively, that had increased significantly compared to the control (38.9 ± 1.3 mg/dl) (P < 0.05). Total protein levels were 2 ± 0.1, 1.9 ± 0.1 and 1.9 ± 0.1 mg/dl, at 1 mg/l concentration of Cu (NO3)2,in 48, 72 and 96 hours, which had decreased significantly compared to the control (2.1 ± 0.1 mg/dl). Based on the obtained results, copper nitrate affected biochemical indices in blood of ship sturgeon and showed severe toxicity at higher concentrations. Manuscript profile
      • Open Access Article

        2 - The effect of copper on some hematological indices in Ship sturgeon (Acipenser nudiventris)
        A. Mohamadrezayei S. Abdali A. Yousefi Jourdehi Z. Salari
        To study the effect of copper toxicity on some hematological indices of Acipenser nudiventris, a group of 120 fish were exposed to different concentrations of copper nitrate (Cu(NO3)2) (0, 1, 5, 10 mg/lit). The results showed that the level of hemoglobin (Hb) was increa More
        To study the effect of copper toxicity on some hematological indices of Acipenser nudiventris, a group of 120 fish were exposed to different concentrations of copper nitrate (Cu(NO3)2) (0, 1, 5, 10 mg/lit). The results showed that the level of hemoglobin (Hb) was increased with the increase in the concentration of copper and also time (P < 0.05). For the concentrations of 5 and 10 mg/lit, the numbers of red blood cells (RBC) were considerably reduced after 24 hours (P < 0.05). After 24 hours, the percent of hematocrit (Hct) was increased and the number of white cells (WBC) was decreased, and the volume of red blood cells (MCV) was considerably increased for all concentrations (P < 0.05). The average of hemoglobin concentration (MCHC) did not change and was the same in all concentrations after 24 and 48 hours (P ≥ 0.05) but was increased considerably after 72 and 96 hours (P < 0.05). The average of hemoglobin concentration (MCH) was increased considerably in 10 mg/lit concentration after 24, but was increased considerably in 1 mg/lit after 72 and 96 hours (P < 0.05). The results of differentiation counting of leucocytes showed that with increase in copper concentration and time, the number of lymphocytes in the 5 and 10 mg/lit concentrations were decreased considerably after 24 hours, but were increased in 1 mg/lit concentration after 48 hours (P < 0.05). The number of monocytes was considerably decreased after 24 and 48 hours in all concentrations, but was considerably increased in 1 mg/lit after 72 hours (P < 0.05). The number of eutrophil was considerably increased in 5 and 10 mg/lit after 24 hours, but was considerably decreased in 1 mg/lit after 48 and 72 hours (P < 0.05). The study showed that although the copper is an essential element for most of physiologic processes but it showed severe toxic effects on the Acipenser nudiventris, Manuscript profile