Effect of copper nitrate on some physiological and biochemical indices in ship sturgeon (Acipenser nudiventris)

Subject Areas : Marine Chemistry

A. Mohamadrezayei ¹ , S. Abdali ² , A. Yousefi Jourdehi ³

1 - Dept. of Marine biology, Faculty of Marine Science and Technology, Islamic Azad University Tehran North Branch
2 - Dept. of Marine biology, Faculty of Marine Science and Technology, Islamic Azad University Tehran North Branch
3 - International Sturgeon Research Institute, Agricultural Research, Education and Extension Organizaton (AREEO), Rasht, Iran.

Received: 2018-03-21 Accepted : 2018-03-21 Published : 2022-03-21

Keywords: Toxicity, Biochemical indices, Copper nitrate, Acipenser nudiventris,

Abstract :

In order to evaluate copper nitrate (Cu (NO3)2) effects on some blood biochemical indices in Acipenser nudiventris, 120 fish were exposed to 0, 1, 5 and 10 mg/L of Cu (NO3)2  in 12 aquariums with  a volume 100 liters. Blood samples were collected from fish at 24, 48, 72 and 96 hours. The highest percentage of mortality of up to 24 hours after exposure to the toxin was related to concentrations of 10 (70 percent) and 5 (50%) and 1 (10%) mg/l, respectively. After 24 hours, fish died at 5 and 10 mg/l of copper. However, at concentration of 1 mg/L up to 96 hours almost 20 percent of fish were resistant. No mortality was observed in the control group. Triglyceride levels at concentrations of 10 mg/l Cu (NO3)2, was 479 ± 7.7 mg/dl after 24 h and decreased significantly in comparison with the control (709.3 ± 69.3 mg/dl) and at 1 mg/l concentration of Cu (NO3)2 in 48, 48, 72 and 96 hours it reached 237.9 ± 4.2, 23.5 ± 0.8 and 226 ± 0.6 mg/d, respectively (P < 0.05). Cholesterol level at concentrations of 5 and 10 mg/l Cu(NO3)2, was 206.6 ± 7.7 and 229.3 ± 0.6 mg/dl, respectively, after 24 hrs, which had increased significantly compared to the control and 1 mg/l Cu(NO3) concentration (P < 0.05). Glucose levels, at 1 mg/l concentration of Cu (NO3)2 , were 81.5 ± 1.4, 73 ± 1 and 69.3 ± 0.6 mg/dl in 48, 72 and 96 hours, respectively, that had increased significantly compared to the control (38.9 ± 1.3 mg/dl) (P < 0.05). Total protein levels were 2 ± 0.1, 1.9 ± 0.1 and 1.9 ± 0.1 mg/dl, at 1 mg/l concentration of Cu (NO3)2,in 48, 72 and 96 hours, which had decreased significantly compared to the control (2.1 ± 0.1 mg/dl). Based on the obtained results, copper nitrate affected biochemical indices in blood of ship sturgeon and showed severe toxicity at higher concentrations.

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