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Open Access Article
1 - اثر ضد باکتریایی نانو کامپوزیت‌های نقره/لاتریت و نقره/لاتریت/کیتوزان بر روی باکتریهای ایجادکننده عفونت در زخم های پوستی
نازیلا فرهنگی قلعه جوقی محمد رضا فرهپور مجتبی محمدی سعید جعفری راد ساناز مهمازی -
Open Access Article
2 - CuFe2O4@MIL-101(Fe)/GO Nanostructure: Synthesis, Characterization and Investigation of its Drug Delivery Activity
F. Shateran M.A. Ghasemzadeh S.S. Aghaei -
Open Access Article
3 - Phytochemical examination of the hydroalcoholic extract of Polylophium involucratum (Pall.) Boiss. harvested from the heights of the Javaherdeh - Ramsar and determination of its cytotoxic effects on chronic myeloid leukemia
Shahab Ojani Naser Montazeri Masoud Mohammadi Zeydi Masoud GhaneIntroduction: Nowadays, the emergence of allergies, side effects of drugs, antibiotic-resistant strains, and the need of body to antioxidants emphasized the importance of herbal medicines.Aim: Therefore, this project aims to investigate the phytochemical extract of hydr MoreIntroduction: Nowadays, the emergence of allergies, side effects of drugs, antibiotic-resistant strains, and the need of body to antioxidants emphasized the importance of herbal medicines.Aim: Therefore, this project aims to investigate the phytochemical extract of hydroalcoholic of Polylophium involucratum harvested from the heights of the Javaherdeh - Ramsar and determine its cytotoxic effects on chronic myeloid leukemia.Material and methods: To this end, Polylophium involucratum seeds were collected from the heights of the Javaherdeh, Ramsar, and extracted using a microwave assisted extraction. Then, using quantitative and qualitative phytochemical tests, determining antibacterial activity by disk emission method and finally, assessing the effect of cytotoxicity on the category of K562 cancer cells using the MTT method was investigated.Results: Phytochemical screening of the hydroalcoholic extract of Polylophium involucratum confirmed the presence of secondary metabolites such as flavonoids, terpenoids, coumarins, cardiac glycosides, tannins, phenols, quinones, and saponins. The total amount of phenolic compounds and flavonoid compounds was calculated as 12.93±2 and 7.58±7 mg/ml respectively. The percentage of free radical inhibition was obtained at 57.70±0.5 and the IC50 value was 0.66 μg/ml. The aura diameter of non-growth was observed in Gram-negative Escherichia coli bacteria and Gram-positive Staphylococcus aureus and Bacillus cereus 25, 10, and 10 mm respectively. The results of cellular toxicity also showed that Polylophium involucratum hydroalcoholic extract is dose-dependent, with the highest toxicity effect at a concentration of 50 μg/ml, and the highest bioavailability effect at a concentration of 20 μg/ml in 24 hours, with an IC50 level equal to 50.35±0.03 μg/ml with a meaningful level of 5%.Conclusion: Based on the obtained results, in general, the use of effective plant compounds is considered one of the most effective strategies in the treatment of cancer, and the secondary metabolites in Polylophium involucratum extract can be a promising method in the treatment of cancer, which requires more studies and experiments are to be done in the future. Manuscript profile -
Open Access Article
4 - A comprehensive review on the structure, properties and application of phycocyanin pigment
R. Safari S. Reyhani Poul Sakineh YeganehThis review aims to evaluate the intrinsic properties and different applications of phycocyanin pigment in the food and pharmaceutical industries. In addition, in each section, a detailed history of the research conducted in the field of phycocyanin is presented. Phycoc MoreThis review aims to evaluate the intrinsic properties and different applications of phycocyanin pigment in the food and pharmaceutical industries. In addition, in each section, a detailed history of the research conducted in the field of phycocyanin is presented. Phycocyanin is a blue pigment, a light receiver with antioxidant and fluorescent properties in cyanobacteria, and two algae from the genus rhodophytes and cryptophytes. Phycocyanin pigment consists of two relatively similar subunits α and β. The alpha chain contains one phycocyanobilin attached to cysteine 89 and the beta chain contains two phycocyanobilins attached to cysteines 84 and 155. Phycocyanin is commercially produced from spirulina algae (spirulina platensis), in the form of photoautotrophic cultures, and open environments in large ponds or pools in tropical or subtropical areas at the edges of oceans. This pigment can be extracted from the mentioned algae by using different techniques such as enzymatic method, ultrasound, freezing-defrosting, mineral solvent, homogenization, stress and osmotic shock, high hydrostatic pressure, ultracentrifuge, and ultra homogenization. Depending on the extraction conditions, each method has its advantages and disadvantages, but the enzymatic and ultrasound methods are more efficient than other methods. Phycocyanin has many medicinal and therapeutic properties, including anti-cancer and anti-inflammatory; In addition, the positive effect of this pigment on nerve cells, kidneys, and the immune system has been confirmed. Phycocyanin, having three colorings, antioxidant and antimicrobial properties, has the potential to be used in various food formulations, such as yogurt, cheese, ice cream, etc., which have been proven in various research. Manuscript profile -
Open Access Article
5 - ترکیبات شیمیایی و اثر ضدباکتریایی اسانس گیاه بومادران جمع آوری شده از شهرستان خوی (Achillea wilhelmsii C. Koch)
علی امین خانی -
Open Access Article
6 - Antibacterial Effects of Methanolic Extract of Myristica fragrans against Klebsiella pneumoniae and Acinetobacter baumannii Producing broad-spectrum beta-lactamase
Elham Nikouie Ashraf KariminikThe rise of various strains of antibiotic-resistant bacteria has become [E1] one of the primary concerns. Therefore, efforts to utilize plant-derived drugs against drug-resistant bacteria have gained particular importance. This study aimed to investigate the antibacteri MoreThe rise of various strains of antibiotic-resistant bacteria has become [E1] one of the primary concerns. Therefore, efforts to utilize plant-derived drugs against drug-resistant bacteria have gained particular importance. This study aimed to investigate the antibacterial effects of the methanol extract of Myristica fragrans against Klebsiella pneumoniae and Acinetobacter baumannii isolates that produce broad-spectrum beta-lactamases. The plant extract was prepared using the maceration method. Then, the extract was filtered through Whatman filter paper, Grade 1, and concentrated and dried using a rotary evaporator system. Concentrations of 80, 40, 20, 10, 5, 2.5, 1.25, and 0.625 mg/mL of the extract were prepared in a 1:1 v/v mixture of dimethyl sulfoxide and methanol as solvents. Beta-lactamase-producing isolates were identified using the phenotypic method with the antibiotic's cefotaxime and the combination of cefotaxime/clavulanic acid. The agar well diffusion method assessed the antibacterial activity against the isolates. Based on the results, 33% of the Klebsiella isolates and 50% of the Acinetobacter isolates were found to produce beta-lactamase. All of the isolates were sensitive to the methanol extract of Myristica fragrans, with an average minimum inhibitory concentration of 10 and 5 mg/mL, respectively. According to the findings, it can be inferred that the Myristica fragrans extract can inhibit Klebsiella and Acinetobacter isolates in vitro. Therefore, with further research and identification of active compounds, it may be possible to utilize this extract as a potential alternative to antibiotics for treatment in the future. Manuscript profile -
Open Access Article
7 - مقایسه تاثیر آنتی باکتریال عصاره های Schrophularia striata Boiss و Stachys schtschegleevii Sosn روی پاتوژن های جدا شده از عفونت های دستگاه ادراری
سمیه اعظمی بیژن فهیمی مهدی باقری شبنم محسن زادهBackground & Aim: The present study was aimed to evaluate and compare the antibacterial activity of Schrophularia striata Boiss. andStachys schtschegleevii Sosn. Extracts against seven clinical isolates. The test isolates were Streptococcus agalactiae, Enterococcus MoreBackground & Aim: The present study was aimed to evaluate and compare the antibacterial activity of Schrophularia striata Boiss. andStachys schtschegleevii Sosn. Extracts against seven clinical isolates. The test isolates were Streptococcus agalactiae, Enterococcus faecalis, Staphylococcus saprophyticus, E. coli, Klebsiella pneumoniae, Proteus mirabilis and Enterobacter dissolvens. E. coli (ATCC 25922) and S.aureus (ATCC 29213) were used as quality control strains. Experimental: Clinical isolates were identified using standard microbiological methods.The methanol extracts from the aerial parts of these plants were assessed using agar well diffusion and broth microdilution methods. Considering the wide application of ciprofloxacin in treatment of Urinary Tract Infections (UTIs), susceptibility test was performed towards antibiotic. Results & Discussion: The methanol extracts of Schrophularia striata and Stachys schtschegleevii exhibit both high bacteriostatic and bactericidal activities. The Minimum Inhibitory and Bactericidal Concentration (MIC and MBC) values of Schrophularia striata extract ranged between 0.39-12.5 mg/ml and 0.78-25 mg/ml, respectively. The extract of Stachys schtschegleevii was effective in MIC and MBC ranges of 1.56-12.5 mg/ml and 3.12-50 mg/ml, respectively. On comparing the efficiency of the two extracts, Stachys schtschegleevii extract exhibited the highest activity against gram-negative bacteria, whereas, the extract of Schrophularia striata inhibited the growth of Streptococcus agalactiae and Staphylococcus spp. Industrial and practical recommendations: On considering antibacterial activity of both the extracts, these herbal extracts may treat urinary tract infections caused by some of the test isolates. Manuscript profile -
Open Access Article
8 - ارزیابی فعالیت ضد باکتریایی عصاره متانولی گیاه کک گریز در دو مرحله برداشت بر روی تعدادی از باکتریهای هوازی عامل عفونت های واژینال
لیلا فیروزیان لیلا امجد منیر دودیBackground & Aim:Vaginal disease has always been a great concern of women. Medicinal plants as natural resources with fewer side effects can effectively treat these infections given the diversity of these infections, resistance to antibiotics and side effects of exi MoreBackground & Aim:Vaginal disease has always been a great concern of women. Medicinal plants as natural resources with fewer side effects can effectively treat these infections given the diversity of these infections, resistance to antibiotics and side effects of existing drugs. The present study aimed to compare antimicrobial effects of methanol extract of Francoeuria Undulata on growth of several gram-positive and gram-negative bacteria causing vaginal infections. Experimental: Francoeuria Undulata as an indigenous Iranian plant was collected in two harvest times (T1 and T2). Methanol extract of aerial parts of the plant was prepared. The effects of various concentrations (62.5, 125, 250 and 500 mg/ml)of methanol extracts were studied onKlebsiella and Enterobacter as gram-negative pathogens. Minimum Inhibitory Concentration (MIC) of bacterial growth and Minimum Bactericidal Concentration (MBC) were determined using macro-dilution method. Data analysis was performed using ANOVA. Results: The results showed that methanol extracts of aerial parts of Francoeuria Undulata inhibit bacterial growth of Klebsiella pneumoniae and Enterobacter aerogenes. MICs varied from 125mg/ml to 250 mg/ml in the old and new harvest times. Therefore, the smallest diameter of inhibition zone belonged to Klebsiella pneumoniae at 62.5mg/ml concentration in the old harvest time and the largestdiameter of inhibitory zone belonged tothe second strains of Klebsiellapneumoniae at 500mg/ml concentration in the new harvest time. Recommended applications/industries: The findings of the study indicated that methanol extracts of Francoeuria Undulata at two old and new harvest times had strong antibacterial and inhibitory effects on gram-negative bacteria causing vaginal infections. Manuscript profile -
Open Access Article
9 - Synthesis analysis and antibacterial activity of selenium nanoparticles produced by Pseudomonas alcaligenes
morahem ashengroph Seyedeh Roya HosseiniBackground & Objectives: Selenium nanoparticles have a wide range application in industry, biomedical and environmental fields due to their unique physical, chemical and photoelectrical properties. This study was aimed to use aquatic bacteria in bioreduction of sele MoreBackground & Objectives: Selenium nanoparticles have a wide range application in industry, biomedical and environmental fields due to their unique physical, chemical and photoelectrical properties. This study was aimed to use aquatic bacteria in bioreduction of selenite oxyanioninto elemental nano-selenium. Materials & Methods: Synthesized selenium nanoparticles were characterized by spectroscopic analysis and electromicrographs prepared by scanning electron microscopy (SEM). The efficacy of the antimicrobial activity of the synthesized selenium nanoparticles against some Gram-positive and Gram-negative human pathogenic bacteria was also investigated by the agar well diffusion test. Results: Sixteen selenite-resistant bacterial strains were isolated based on selective enrichment techniques in Tryptic Soy Agar (TSA) medium containing 5 mM selenite ion. Our results showed that Pseudomonas alcaligenes SR5 coastal seawater isolate can reduce selenite oxyanion into selenium nanoparticles. Furthermore, the results showed that extracellular selenium nanoparticles with an average size of 36 nm were formed in an optimum selenite concentration of 3 mM and an optimum initial biomass concentration of 15 g/l, following 96 h incubation at 25ᵒ C at (200 rpm under resting cell condition. Conclusion: The current study is the first report on extracellular synthesis of selenium nanoparticles using P. alcaligenes. The produced bio-nanoparticles showed a growth inhibitory effect against four tested pathogenic bacterial strains. Manuscript profile -
Open Access Article
10 - Molecular identification of lantibiotic- producing Bacillus toyonensis isolated from soil and evaluation of its antibacterial activity
Dorna Karami Arekhloo Mohammad Mehdi MahmoodiBackground & Objectives: Lantibiotics are small peptide antibiotics that produced by a large number of gram positive bacteria to limit growth of other bacteria. This study was performed with the aim of isolation and molecular identification of lantibiotic producer B MoreBackground & Objectives: Lantibiotics are small peptide antibiotics that produced by a large number of gram positive bacteria to limit growth of other bacteria. This study was performed with the aim of isolation and molecular identification of lantibiotic producer Bacilli from soil, also to optimize the production conditions and antibacterial activity of the product. Materials & Methods: In this basic research, soil samples were collected from green bean fields located at Kazerun. The vegetative cells were killed by pasteurization method and the Gram positive spore former colonies were selected for further studies. These bacteria were grown in the broth media and their culture extracts were examined for lantibiotic production by well diffusion method. Appropriate colonies with high ability for lantibiotic production were selected and identified by biochemical and molecular methods. Also lantibiotic production at different temperatures and pH values was investigated. Results: The isolated strain was identified as Bacillus toyonensis based on DNA sequencing. Maximum lantibiotic production was determined after 24h incubation at 30°C and pH 7. These results showed that the addition of 1% glucose and pepton can improve lantibiotic production. The purified lantibiotic showed inhibitory effects on some gram positive bacteria. Conclusion: According to ability of this isolated strain in lantibiotic production, further studies is required for more accurate identification of produced lantibiotic in order to be employed in pharmaceutical applications. Manuscript profile -
Open Access Article
11 - Antibacterial potential of five lichen species from Arasbaran region against Dikerya chrysanthemi potatoes rot causal agent in the laboratory and storage condition
Seyedeh Maryam Shahidi Soleiman Jamshidi Mohammad TorabiPotato is one of the important vegetables which is tremendously getting damaged by Dikerya chrysanthemi causing soft rot during storage. In order to utilize lichens antibacterial potential to the disease management, five lichen species collected from Arasbaran region in MorePotato is one of the important vegetables which is tremendously getting damaged by Dikerya chrysanthemi causing soft rot during storage. In order to utilize lichens antibacterial potential to the disease management, five lichen species collected from Arasbaran region including Pleopsidium gobiensis, Ramalina sinensis, Parmelina tiliacea, Anaptychia setifera and Lecanora argopholis were extracted using ethanol, acetone and diethyl ether as solvents and bioassayed in laboratory condition by disc diffusion, minimal inhibitory and bactericidal concentration (MIC and MBC) methods. Pleopsidium gobiensis methanolic extract and Parmelina tiliace diethyletherextract were the most effective treatments against the bacterium with 7.9 and 6.6 mm of inhibition zone and 0.54 and 1.09 mg/ml of MIC and MBC, respectively. They were even more effective than Streptomycin which were used as positive controls. On the whole, P. tiliacea and P. gobiensis had the highest inhibitory effects on bacterial growth. In storage condition, spraying injured potato tubers with bacterial suspensionbefore or after selected lichens separate inoculations showed that all lichen treatments had protective and curative potential effects on bacteria, being able to save significant part of potato tubers from bacterial damage in comparison to the control. The most effective treatment was diethyl ether extract of R. sinensis applied after bacterial inoculation which could protect 91% of potato tubers from bacterial damage. Manuscript profile -
Open Access Article
12 - The effect of Cuminum cyminum, acidity, temperature and inoculums’ level on the growth of Xanthomonas campestris
Nazanin Miri Roya Rezaeian-Doloei Reza Sadrabadi HaghighThe Xanthomonas genus is one of the most important groups of plant pathogenic bacteria that cause post-harvest spoilage. Substantial crop losses may result from the rapid spread of the bacteria under favorable conditions, lack of seed germination, seedling death and vas MoreThe Xanthomonas genus is one of the most important groups of plant pathogenic bacteria that cause post-harvest spoilage. Substantial crop losses may result from the rapid spread of the bacteria under favorable conditions, lack of seed germination, seedling death and vascular obstruction of plant. The aim of this study was to investigate the combined effects of different concentrations of Cuminum cyminum essential oil (EO; including 0, 0.2, 0.4 and 0.8%), three levels of acidity (5, 6 and 7), two inoculums’ level (103 and 105 CFU/ml) and two incubation temperatures (26 and 28˚C) on the growth of X. campestris in the nutrient broth medium in a completely randomized design with three replications. Growth was monitored by visible turbidity during a 30-day period. The minimum inhibitory concentration and minimum bactericidal concentration of EO against X. campestris was 1 and 2% respectively. According to the results, P-cuminaldehyde was the main component, with a content of 30.5%. The statistical analysis of data showed that the maximum time to detection of bacteria (19 days) in the concentrations of 0.8% EO, pH of 5, the inoculum level of 103 CFU/ml and incubation temperature of 26˚C and the minimum time to detection (16 hours) in the inoculums level of 105 CFU/ml, pH of 7, 28 ˚C and no EO was observed. In addition to the concentration of Cuminum cyminum EO as an antimicrobial agent, acidity of medium is also considered the factors influencing the growth of X. campestris. By decreasing the pH, the time-to-detection of bacteria was increased. In conclusion, using a combination of different factors can inhibit the growth of bacteria, significantly. Manuscript profile
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