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Open Access Article
1 - The co-assessment of antioxidant capacity and flavonoids levels of alcoholic extract of some of current traditional medicine plants
فاطمه کریم نژاد فهیمه فیروزبخت صدیقه میرلطیفی شهرزاد قدیمی فاطمه فرجادمندBackground: In the last years, there has been growing interest in finding plants for treatment andprevention of disease. Borage officinalis, Camellia sinensis, Chamaemelum nobile, Morus albaand Rosmarinus officinals are usually used in traditional medicine. The aim of t MoreBackground: In the last years, there has been growing interest in finding plants for treatment andprevention of disease. Borage officinalis, Camellia sinensis, Chamaemelum nobile, Morus albaand Rosmarinus officinals are usually used in traditional medicine. The aim of this experimentalstudy was co assay of antioxidant and flavonoids levels of alcoholic extraction of these plants. Inthis study the antioxidant activity was surveyed by applying iron ion reducing assay (FRAPassay) and flavonoids levels with measuring the complex flavonoids with trivalent aluminumion. Results from the present study show,the alcoholic extraction of Rosmarinus officinals havea marked antioxidant activity, and the high levels of flavonoids was recorded by Chamaemelumnobile. Camellia sinensis had both effects; antioxidant activity, flavonoids levels. The using ofscreening tests such as the mentioned methods are useful for finding biological components withdifferent effects Manuscript profile -
Open Access Article
2 - Evaluation of the Antioxidant Activity of Some Rosaceae Plants as an Alternative to the Synthetic Antioxidants in Food Industry
فائزه Kamali A.R Sadeghi Mahonak زهرا Nasiri farIntroduction : The applications of natural antioxidants with medicinal values are growing in the preventive medicine and food industries. This study investigates the antioxidant ability of five species of Rosaceae family and compares the activities with the synthe MoreIntroduction : The applications of natural antioxidants with medicinal values are growing in the preventive medicine and food industries. This study investigates the antioxidant ability of five species of Rosaceae family and compares the activities with the synthetic antioxidants BHT, BHA as well as the natural ascorbic acid. Materials and Methods : In this study, the phenolic content of the methanolic extract of the samples were isolated and the antioxidant activities of five species of Rosaceae were determined by using DPPH free radical and nitric oxide scavenging , and the results were compared to the synthetic antioxidant Results : The results showed that the content of studied plant samples ranged from 0.51±0.41 – 35.53±0.89 mgGAEg -1. Concentraition of extract for scavening 50 percent of free radicals and nitric oxide radicals varied from 77.42 ± 0.91 to 1484.2 ± 96.44 and 233.24±3.39- 1572.46±203.76 Ug/ml. The highest phenolic amount was related to the Rosa damascena Mill leaf and next grade Cydonia oblonga Mill leaf. Extract of damask Rose, Quince leaves, damask Rose petals, Quine fruit and seed and Almond seed had high antioxidant activity and no significant difference was observed between them and the synthetic antioxidants (BHT, BHA and Vit C). Conclusion : The results of this study clearly suggested that there is a significant relationship between the antioxidant activity and the phenolic concentration and the extracts of damask Rose, Quince leaves, damask Rose petals, Quince fruit and seed and almond seed might be employed as appropriate alternatives to the synthetic antioxidants. Manuscript profile -
Open Access Article
3 - بررسی اثر مکمل‌های غذایی و باکتری سودوموناس پوتیدا (Pseudomonas putida) جهت بهبود برخی صفات کمی و کیفی قارچ دکمه‌ای (Agaricus bisporus)
فرشته ماکنالی عبدالکریم کاشی رضا صالحی محمدی احمد خلیقی -
Open Access Article
4 - Effect Of Exhaustive Exercise and Supplement N-Acetyl Cysteine on TAC, MDA and CRP in Wistar Rats after a Single bout Of Exhaustive Exercise
Ali Aalizadeh Najmeh MobseriInroduction and ObjectivePhysical activity exhausting the release of reactive oxygen species (ROS) and nitrogen species activated (RNS) is a well has a dual role as both deleterious and beneficial species is known. N - Acetylcysteine (NAC) acetylating of the amino-L-cys MoreInroduction and ObjectivePhysical activity exhausting the release of reactive oxygen species (ROS) and nitrogen species activated (RNS) is a well has a dual role as both deleterious and beneficial species is known. N - Acetylcysteine (NAC) acetylating of the amino-L-cysteine, which is converted in the body into metabolites that are capable of stimulating glutathione synthesis may be resulting in the elimination of free radicals. Effective properties of complementary N – acetylcysteine reduces fatigue and effects on sub maximal contractions.Material and Methods: This study used 16 female Wistar rats weighing 150 to 200 g and (8 to 10 weeks old) were divided into 2 groups. First group effervescent tablets were given (600 mg) of N-acetyl cysteine dissolved in water) four hours before the experiment by gavages and group was taken as the control. Blood samples were taken 1 hour before starting the exhaustive exercise - immediately after the exhaustive exercise and after one hour at rest.Results: Significant decrease was observed in the concentration of MDA immediately after the exhaustive exercise in the group consumed NAC and blood concentration of TAC one hour after exhaustive exercise as compared with the control (P≤0.05). Reactive protein concentrations - C (CRP) in the period before, immediately and after exhaustive exercise did not show any significant difference.Conclusion:The results of our study demonstrated that orall administration of N-acetyl cystein at least four hours before an exhaustive exercise can significantly reduce the harmful effects of oxidative stress in rats. Manuscript profile -
Open Access Article
5 - Determination of total antioxidant capacity with nanoparticles
Safieh MomeniTotal antioxidant capacity (TAC) as the cumulative activity of antioxidants in a sample is an important parameter in the analysis of biological or food matrices. Therefore, it is very important to evaluate the total antioxidant capacity of the substances in the diet and MoreTotal antioxidant capacity (TAC) as the cumulative activity of antioxidants in a sample is an important parameter in the analysis of biological or food matrices. Therefore, it is very important to evaluate the total antioxidant capacity of the substances in the diet and biological fluids. Based on this, many methods check their antioxidant capacity and effectiveness in different conditions. However, there is often no strong correlation between the capacities measured on the same materials with different methods, which is due to the variety of active materials, mechanisms and different characteristics such as different types of antioxidants, the presence of other interfering substances in the sample, lack of participation of antioxidants are used in the method reaction. In recent years, different analytical methods based on nanoparticles have been developed to determine the antioxidant capacity of foods and plant materials.In these measurement methods, nanoparticles such as gold, silver, iron oxide, manganese oxide, quantum dots and cerium oxide have been used. In this article, we review some of the researches conducted in the field of total antioxidant capacity measurement. Manuscript profile -
Open Access Article
6 - The effect of zinc-methionine supplementation on antioxidant status and expression of interleukin-4 and interleukin-6 genes in female rats under heat stress
Matin Jamei Ali Asghar Sadeghi Mohammad ChamaniIntroduction:During heat stress, the animal body requires more antioxidant compounds and activity of antioxidant enzymes. Zinc plays a role in the structure and activity of antioxidant enzymes. One of the ways to supply the zinc requirement, is to use organic zinc suppl MoreIntroduction:During heat stress, the animal body requires more antioxidant compounds and activity of antioxidant enzymes. Zinc plays a role in the structure and activity of antioxidant enzymes. One of the ways to supply the zinc requirement, is to use organic zinc supplements, which zinc combined with methionine, and have more intestinal absorption Aim: This study was done to evaluate the effects of different doses of zinc-methionine supplementation on the blood antioxidant status and the expression of interleukin 4 and 6 genes in rats exposed to heat stress.Materials and methods: In a completely random design, 20 female rats were divided into four treatment groups with five replicates. Rats were kept at a temperature of 31±2 °C for 20 hours and at a temperature of 38±2 °C for 4 hours per day (to create heat stress). The rats in the control group were fed standard pellets without additives, and the three experimental groups were fed standard pellets plus 15, 30 and 45 mg zinc-methionine supplement per kilogram of dry matter for 30 days. At the end of the experiment, a blood sample was collected from the inferior vena cava. Total antioxidant capacity, malondialdehyde concentration, antioxidant enzymes, liver enzymes in the serum and the expression of interleukin-4 and interleukin-6 genes were measured.Results: Serum zinc concentration increased linearly with increasing zinc dose in the diet (P<0.05). The serum activity of two enzymes, alanine aminotransferase and aspartate aminotransferase decreased linearly with increasing zinc-methionine dose in the diet (P<0.01). The highest activity of glutathione reductase and glutathione peroxidase was observed in the group receiving 30 mg/kg, and the lowest activity of these enzymes was observed in the control group. In general, the relative expression of the interleukin-4 gene was increased, and the relative expression of the interleukin-6 gene was decreased (P<0.05). The highest expression of interleukin-4 gene belonged to the group receiving 30 mg, and the highest relative expression of interleukin-6 gene belonged to the control group and the group receiving 15 mg/kg.Conclusion: The results of this study showed that the dose of up to 30 mg of zinc-methionine per kg of diet used in this study increases the activity of superoxide dismutase enzyme and glutathione peroxidase enzyme activity, decreases the activity of liver enzymes in the serum, increases the expression of the anti-inflammatory gene (Interleukin-4) and decreases the expression of inflammatory gene (Interleukin-6), which indicates the reduction of oxidative stress and the reduction of the effects of heat stress in the body of the rat. Manuscript profile -
Open Access Article
7 - The effects of piperine supplementation on colony population, body weight after emerging, viability and vitellogenin gene expression in honey bees under heat stress
Zahra Farhadi Ali Asghar Sadeghi Farahnaz Motamedi Sedeh Mohammad ChamaniAim: This study was done to evaluate the effects of piperine (phytogenic material in pepper) supplementation on antioxidant capacity, vitellogenin gene expression, the viability and body weight of honey bees at the time of emerging.Materials and methods: 32 bee hives we MoreAim: This study was done to evaluate the effects of piperine (phytogenic material in pepper) supplementation on antioxidant capacity, vitellogenin gene expression, the viability and body weight of honey bees at the time of emerging.Materials and methods: 32 bee hives were divided to 4 treatment groups and 8 replications in a completely random design. The environment temperature of region was 38 degrees Celsius for at least 4 hours per day. The control group received syrup without piperine, and the treatment groups received syrup containing 2000, 4000 and 6000 μg of piperine per liter, respectively. The level of malondialdehyde and antioxidant capacity of the body, weight and body composition of bees after emerging, colony population and relative expression of vitellogenin gene were determined.Results: Supplementation of 2000 and 4000 μg piperine per liter of syrup caused an increase in body weight, a decrease in the level of malondialdehyde and increase in antioxidant capacity. The addition of piperine in different doses caused a significant increase in vitellogenin gene expression compared to the control group. The spawning level and colony population that received 6000 μg/liter piperine was lower than the group that received 2000 and 4000 μg/liter P<0.05).Conclusion: Based on the results, supplementation of 2000 to 4000 μg/liter of piperine has a positive effect on increasing the antioxidant capacity, vitellogenin gene expression, body weight of new-emerged bees and colony population, and a higher dose causes a decrease in spawning rate and reduction of honey bee population. Manuscript profile -
Open Access Article
8 - Investigating relative water content, electrolyte leakage, phenolic compounds and activity of phenylalanine ammonia-lyase and tyrosine ammonia-lyase enzymes in triticale leaves treated with sodium chloride and calcium chloride
Malihe Jahani Mohammad Reza Hadi Mojtaba Jafarinia Sedighe JahaniIntroduction: Considering the increasing number of saline lands due to water scarcity, it seems necessary to investigate ways to increase tolerance to salinity stress. Also, in order to overcome the negative effect of salinity stress, the use of calcium ion supplement i MoreIntroduction: Considering the increasing number of saline lands due to water scarcity, it seems necessary to investigate ways to increase tolerance to salinity stress. Also, in order to overcome the negative effect of salinity stress, the use of calcium ion supplement in the growth environment is very useful and practical to improve the stress factor.Objective: The purpose of this research was to investigate relative water content, electrolyte leakage, amount of mineral elements, antioxidant capacity, phenolic compounds and activity of phenylalanine ammonia-lyase and tyrosine ammonia-lyase enzymes in leaves of triticale (x Triticosecale Wittmack) treated with sodium chloride and calcium chloride.Materials and methods: This experiment was conducted as a completely randomized design with 3 replications in controlled greenhouse conditions. One-week-old seedlings were exposed with sodium chloride doses (0, 50, 100 and 150 mmol L-1) and calcium chloride doses (0, 6 and 10 mmol L-1) for 5 weeks and then some physio-morphological and biochemical characteristics including leaf number and surface, relative water content, ion leakage, contents of phenolic compounds (anthocyanin, total phenol, total flavonoid, total flavonol), enzymes activity (phenylalanine ammonia-lyase, tyrosine ammonia-lyase, glutathione reductase, glutathione S-transferase), antioxidant capacity and amount of mineral elements (sodium, potassium, calcium) were measured in leaves.Results: The results showed that salt stress significantly decreased the number and surface of leaves, relative water content, activity of glutathione reductase enzyme, the amount of potassium and calcium in leaves, but increased ion leakage, the contents of phenolic compounds, activity of phenylalanine ammonia-lyase, tyrosine ammonia-lyase and glutathione S-transferase enzymes, antioxidant capacity and sodium content in leaves. While the addition of calcium to saline environment increased the number and surface area of leaves, relative water content, activity of glutathione reductase enzyme, the amount of potassium and calcium in leaves, but decreased ion leakage, the contents of phenolic compounds, activity of phenylalanine ammonia-lyase, tyrosine ammonia-lyase and glutathione S-transferase enzymes, antioxidant capacity and sodium content in leaves.Conclusion: Calcium reduced the harmful effects of salinity stress in leaves of triticale plant, so adding calcium to salty soils can be a simple, practical and economic solution to deal with salinity stress and increase soil productivity and a step towards to provide sustainable agriculture. Manuscript profile -
Open Access Article
9 - Evaluation of some quantitative and qualitative properties of cornelian cherry (Cornus mas L.) fruit during the last stages of maturation
mehdi panahi Jafar Hajilou Nader ChaparzadehIn recent years, increasing attention has been paid by consumers to cornelian cherry fruit, which is rich in antioxidants. This research was carried out based on a randomized complete block design for evaluation of the variations in some quantitative and qualitative pro MoreIn recent years, increasing attention has been paid by consumers to cornelian cherry fruit, which is rich in antioxidants. This research was carried out based on a randomized complete block design for evaluation of the variations in some quantitative and qualitative properties of cornelian cherry fruits during the last stages of maturation in 2015. Fruits were harvested at 4 different times and from 4 main geographical directions of the tree and were then immediately transferred to the laboratory. Several parameters such as dimension (length and width), weight, firmness, pH, total soluble solids (TSS), total titratable acidity (TA), TSS/TA ratio, ascorbic acid (vitamin C), total phenolic compounds, total flavonoids, total anthocyanins, and total antioxidant capacity of the fruits were investigated. The results showed that the effect of different harvest dates on all traits was significant (P<0.05) while no significant differences were observed between replications. During fruit ripening, weight, dimension, pH, TSS, TSS/TA ratio, total flavonoids, and total anthocyanins increased while firmness, TA, total phenol, and total antioxidant capacity decreased. The content of ascorbic acid increased during fruits ripening, but it reduced again in the last harvest. The results indicate that the third harvest time (16.63 Brix level) can be the best harvest date for this genotype. Manuscript profile -
Open Access Article
10 - Variation on biochemical, phytochemical and genetic diversity of fig (ficus carica) from East Azerbaijan province
akbar ghorbani hamid Hasanpour sezai ErcisliAbstract. Fig (Ficus cariaca) is native to western and eastern mediterranean regions such as western and northwestern of Iran. Iran is one of the important fig producers in the world. In this study, biochemical, phycochemistry characteristics and genetic diversity of 38 MoreAbstract. Fig (Ficus cariaca) is native to western and eastern mediterranean regions such as western and northwestern of Iran. Iran is one of the important fig producers in the world. In this study, biochemical, phycochemistry characteristics and genetic diversity of 38 fig genotypes from Arsbaran in East Azerbaijan province in the Horticulture Laboratory of Urmia University in 2015 were investigated. Biochemical results showed that, TSS, pH, TSS/TA, vitamin c, antioxidant capacity, total anthocyanin, total flavonoid and total phenol were significant (p ≤ 0.01). Most of the studied biochemical traits indicated high variation. The results of the molecular data showed that a total of 131 bands were scored, of which 121 bands (92%) were polymorphic. The amount of polymorphic (PIC) information for the primers used varied from 0.22 to 0.47 in ISSR7 and ISSR20 respectively. Also, marker index were between 0.15 to 2.5 in ISSR7 and ISSR20 respectively. Based on the results of cluster analysis, the studied fig genotypes were classified into two main groups, so that the most of collected genotypes from Kalibar belonged to second group. So it could be concluded that geographic location of the studied fig genotypes is the main reason for their separation by this marker. Based on obtained results, the genotypes Kh9 and H17 were suggested for breeding programs. Manuscript profile -
Open Access Article
11 - Effects of different concentrations of chitosan bio-stimulant on some physiological and biochemical traits of Andrographis paniculate L.
Mozhdeh Jafari Lafoot Leila Pishkar Daryush TaleiChitosan is a nitrogen containing polysaccharide synthesized naturally by deacetylation reaction of chitin, which is confirmed as an efficient bio-stimulant to improve production of secondary metabolites in medicinal plants. In the present study, the effects of chitosan MoreChitosan is a nitrogen containing polysaccharide synthesized naturally by deacetylation reaction of chitin, which is confirmed as an efficient bio-stimulant to improve production of secondary metabolites in medicinal plants. In the present study, the effects of chitosan (0, 100, 200, 300, and 400 mg/L) on growth, photosynthetic pigments, secondary metabolites, antioxidant capacity, activity of antioxidant enzymes, quantity and quality of protein, and the expression of HMGS and HMGR genes were examined in Andrographis paniculata L. The results showed that application of chitosan significantly increased the chlorophyll and carotenoids and, as a result, increased plant growth and biomass. Chitosan treatments (300 and 400 mg/L) increased the expression of HMGS and HMGR genes in plant leaves, which was associated with a significant increase in the accumulation of total phenols and flavonoids. Also, chitosan improved the activity of superoxide dismutase and peroxidase enzymes and increased the antioxidant capacity of A. paniculata leaves. Examination of the quantity and quality of proteins showed that the intensity of protein bands with the molecular weight of 60 kD under 300 and 400 mg/L chitosan treatment and also the intensity of protein bands with the molecular weights of 35 and 20 kD under 300 mg/L chitosan treatment increased compared to the other concentrations. Therefore, these results revealed that application of chitosan can increase accumulation of secondary metabolites and the antioxidant capacity in the medicinal plant A. paniculata. Manuscript profile -
Open Access Article
12 - بررسی مواد موثره اسانس در سرشاخه‌های گلدار و عملکرد آنتی اکسیدانی گیاه دارویی پونه Mentha longifolia (L.) Huds.در شرایط طبیعی و زراعی در دو مرحله رویشی و زایشی
آرزو مستعد آرین ساطعی معصومه مازندرانی -
Open Access Article
13 - بررسی اثر آنتی اکسیدانی عصاره سنجد (Elaeagnus angustifolia L.) بر ماندگاری آب پرتقال
مریم سروریان افشین جعفرپور -
Open Access Article
14 - The Effect of High-intensity Interval Training and High-Protein Diet on the Total Oxidant Status and Total Antioxidant Capacity in Cardiac Tissue of Obese Male Rats
Azin Ghane Hossein Abednatanzi Marziyeh Saghebjoo Mehdi HedayatiObesity causes oxidative stress and is a risk factor for cardiovascular diseases. Exercises and diet modification can affect oxidant and antioxidant factors. The aim of this study was to investigate the effect of high-intensity interval training (HIIT) and high-protein MoreObesity causes oxidative stress and is a risk factor for cardiovascular diseases. Exercises and diet modification can affect oxidant and antioxidant factors. The aim of this study was to investigate the effect of high-intensity interval training (HIIT) and high-protein diet (HPD) on total oxidant status (TOS), total antioxidant capacity (TAC) and the ratio of TAC to TOS in the heart tissue of obese male rats. Forty 12-week-old obese male rats (Lee index above 310) were divided into five groups of eight including HIIT (5-12 intervals of 30 seconds with an intensity of 90% VO2max, 5 sessions per week, 10 weeks), HPD (about 57.5% of total calories from protein), HIIT+HPD, obese control-1 (OC-1) and obese control-2 (OC-2) and eight non-obese mice were also divided into the non-obese control (NC) group. became After 48 hours from the last intervention session, heart tissue was removed to measure TAC and TOS levels. Data were analyzed using one-way analysis of variance, post hoc Tukey, Kruskal-Wallis and Mann-Whitney tests at a significance level of p ≤ 0.05. The results showed that the TAC level of heart tissue in the HIIT, HPD, HIIT+HPD groups was significantly higher than in the OC-1 group. On the other hand, the TOS level of heart tissue was significantly lower in the HIIT and HIIT+HPD groups than in the HPD and OC-2 groups. Also, the ratio of TAC to TOS was significantly higher in the HIIT and HIIT+HPD groups compared to the OC-2 group. According to the results, a combination of HIIT and HPD improves TAC and TOS of heart tissue. It seems that HIIT together with HPD strengthens the antioxidant system and reduces oxidative stress in obesity conditions. Manuscript profile
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