Background & Objectives: Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen, and one of the mortality causes of nosocomial infections, specifically in patients with severe burns. One of the drug resistance mechanisms in P. aeruginosa is mutation or dow More
Background & Objectives: Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen, and one of the mortality causes of nosocomial infections, specifically in patients with severe burns. One of the drug resistance mechanisms in P. aeruginosa is mutation or down-regulation of oprD gene. In this study, we investigated oprD mutations in imipenem-resistant P. aeruginosa isolates in Gilan province.
Materials & Methods: In this sectional study, 45 P. aeruginosa strains were isolated from different clinical samples of Rasht and Lahijan hospitals and laboratories. The antibiotic resistance and susceptibility of the strains were determined by Kirby Bauer and minimum inhibitory concentration (MIC) methods. Then, PCR and sequencing were performed to evaluate oprD mutation in imipenem-resistant isolates.
Results: 44.4% isolates were imipenem- resistant. The highest resistance was shown to 512 µg/ml of imipenem. Sequencing analysis showed that 5 isolates have T103S and K115T missense mutations in the oprD gene. Furthermore, silencing mutations including L92L were detected in 7 samples, S100S in 7 samples, P116P in 5 samples, and G151G in 3 samples.
Conclusion: Since oprD plays, a major role in imipenem entry to the cell, oprD mutation can be the cause of imipenem resistance in P. aeruginosa isolates. Furthermore, the oprD mutation might have changed the affinity to imipenem in these isolates.
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Background & Objectives: Pseudomonas aeruginosa is a common opportunist pathogen in hospitals and the etiologic agent of the majority of infections in human beings. This study aimed to genotyping of P. aeruginosa isolated from hospital More
Background & Objectives: Pseudomonas aeruginosa is a common opportunist pathogen in hospitals and the etiologic agent of the majority of infections in human beings. This study aimed to genotyping of P. aeruginosa isolated from hospital infections. Materials & Methods: This cross-sectional study was performed on 18 isolates of P. aeruginosa isolated from hospitalized patients in Shahrekord hospitals. We applied three different methods, RAPD-PCR, Rep-PCR and ERIC-PCR for genotyping of the isolates. Results: Based on RAPD-PCR method, overall 86 different bands of DNA with a range of 300 to 1000 bps were obtained from the under study isolates and among them, 74 bands were polymorphic. Analysis of P. aeruginosa isolates based on ERIC-PCR produced 98 bands with a range of 150-8000 bps. Overall 16 genomic profile with 30 to 86% and for a few strains, 100% similarities were produced based on Rep-PCR. Conclusion: Overall, all isolates showed polymorphic band patterns and no monochromic band was observed for the isolates. The presence of polymorphic band patterns in these techniques shows high rates of polymorphism in the genome of P. aeruginosa. Furthermore, the techniques used in this study are reliable approaches for genotyping of P. aeruginosa.
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Background & Objectives: Identifying the bacteria causing the infection and interacting with the immune system is too essential. RAGE is a receptor, which recognizes several endogenous and exogenous molecules and CXCL11 participates in the induction of appropriate i More
Background & Objectives: Identifying the bacteria causing the infection and interacting with the immune system is too essential. RAGE is a receptor, which recognizes several endogenous and exogenous molecules and CXCL11 participates in the induction of appropriate immune responses against microbes. The aim of the study was expression of the molecules in the patients suffering from septicemia versus healthy controls. Materials and Methods: In this case-control study, the expression levels of RAGE and CXCL11 in 40 patients with septicemia and 40 healthy subjects, were evaluated using Real time-PCR technique. Diagnosis of septicemia was performed by blood culture and bacterial biochemical tests. Results: RAGE mRNA levels were significantly increased in patients infected with Pseudomonas aeruginosa compared with Escherichia coli, Staphylococcus aureus and Acinetobacter bamanii. However, no significant increase in CXCL11 expression level was observed in patients and healthy controls and also in comparison with bacteria causing infection. Conclusion: Results showed that RAGE is a critical receptor against Pseudomonas aeruginosa during septicemia, Therefore, methods to reduce the expression of RAGE molecules can play a practical role in cleansing the blood from this bacterium.
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