List of Articles ساختار ژنتیکی

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  1 - Investigation of polymorphism of Markhoz goat microsatellite markers
  Sajad Badbarin Reza Seyedsharifi Hassan Khamis Abadi Javad Ahmadpanah
  The use of molecular markers, especially microsatellite markers provides very valuable information about the existing genetic structure under study. This information can be used to protect an endangered species or to investigate the location of genes affecting quantitat More

  The use of molecular markers, especially microsatellite markers provides very valuable information about the existing genetic structure under study. This information can be used to protect an endangered species or to investigate the location of genes affecting quantitative traits (QTL). The purpose of this study was to emphasized the importance of microsatellite markers for the study of genetic diversity in Markhoz goats and their use in conservation strategies.In this study, blood samples were taken from 240 Markhz goats in Kurdistan province randomly. DNA extraction from whole blood samples was performed by salting out method. Then, the genetic diversity of 30 microsatellite markers was investigated using polymerase chain reaction (PCR). Alleles of each individual were identified using silver staining method. Genetic parameters related to the genetic structure of Markhoz goat were calculated using POPGENE software.Except for INRA040 marker, all markers used were well amplified. Among the amplified markers, MCM136 showed low polymorphism but other markers had high polymorphism. ILSTS030 markers with 6 alleles and MCM136 markers with 3 alleles produced the highest and lowest number of alleles, respectively. ILSTS030 marker showed the highest (0.7504) and MCM136 marker showed the lowest (0.0158) expected heterozygosity.Microsatellite markers seem to be a useful and reliable tool for identifying goat breeds and their use can be a solution to protect endangered breeds. Manuscript profile
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  2 - Investigation of genetic structure of sanjabi sheep using microsatellite markers
  reza sayed sharifi sajad Badbarin nemat hedayat evrigh jamal seif davati sima savar sofla
  Sanjabi sheep is one of the valuable breeds of sheep in the west of the country, which is very important in terms of meat and wool production. Considering the importance of native sheep breeds and their breeding in order to achieve production with more quantity and qual More

  Sanjabi sheep is one of the valuable breeds of sheep in the west of the country, which is very important in terms of meat and wool production. Considering the importance of native sheep breeds and their breeding in order to achieve production with more quantity and quality, identification of genetic structure and estimation of relevant parameters (number of observed and effective alleles, heterozygosity, Shannon index, etc.) The present study was performed. . The study population consisted of 100 sanjabi rams and ewes located in Mehregan station of Kermanshah province that were randomly selected. DNA extraction was performed by salt method. PCR reaction was performed using 10 microsatellite markers. Amplified DNA fragments were stained on acrylamide gel and detected by silver nitrate method. Alleles were scored according to their size and compared to the standard index PUC8 of Fermentase Company. The results of this study showed that all markers were polymorphic. The mean number of observed and effective alleles for all markers was calculated to be 4.5 and 2.9012, respectively. The highest and lowest expected heterozygosity were obtained in OarFCB11 and BMS2721 markers equal to 0.7548 and 0.5619, respectively. The expected heterozygosity for all markers (genetic diversity) was 0.6487. According to the obtained results, it can be said that the studied sheep have a desirable level of diversity and can be achieved by high breeding by breeding and selection of superior livestock. Manuscript profile
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  3 - Molecular Phylogeny of King soldier bream (Argyrops spinifer) in the Persian Gulf and Oman Sea (Bandar Boushehr, Bandar Abbas , Bandar Chabahar)
  shirin valipour Pargol Ghavam Mostafavi Mohammadhassan Shahhosseiny Farhad Kaymaram
  Inroduction & Objective: King soldier bream is one of the species of family Sparidae, which is commercially and ecologically very important in the Iranian waters of the Persian Gulf and Oman Sea. The species of this family are morphologically very similar to each ot More

  Inroduction & Objective: King soldier bream is one of the species of family Sparidae, which is commercially and ecologically very important in the Iranian waters of the Persian Gulf and Oman Sea. The species of this family are morphologically very similar to each other and they may be mistakenly known as another species. Therefore, molecular identification of them has to be considered. Materials and Methods: In this study, the phylogenetic relationship of king soldier bream fish based on mitochondrial region(CO1) was investigated using DNA sequencing method. For this purpose, 90 samples were collected from the soft tissue of the caudal fin using Ammonium acetate, and its quantity and quality were determined by Spectrophotometry and Electrophoresis. Optimal DNA samples for Polymerase Chain Reaction (PCR) and sequencing were examined. After gene amplification and sequencing, Bio Edit version 7.0.1, BLAST, MEGA 7.0.2 and DnaSP 5.10.01 software were used to draw phylogenetic trees. Results: Based on the phylogenetic tree obtained from the sequences, king soldier bream was divided into two main branches in Bandar Abbas, Bandar Bushehr and Bandar Chabahar areas. All samples of the first branch showed a sister relationship with the second branch and all the samples of branches one and two showed sisterhood with samples KJ012292 and kJ012291 from the Gene Bank in Italy with 82, 67 and 86% accuracy. Conclusion: The results can provide useful information on the conservation and management programs, for the preservation, revitalization, and resource of the population this valuable species. Manuscript profile
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  4 - The genetic structure of geographic populations of Chilo suppressalis (Walker) (Lepidoptera: Crambidae) in northern Iran based on mitochondrial COII gene
  Abbas Heydari Alireza Nazari Mohammad Ali Oshaghi Elham Sanatgar
  10.30495/sarj.2023.1977771.1115
  Rice is one of the most important and oldest crop and is one of the three leading crops in the world. Chilo suppressalis (Walker), rice striped stem borer, is a key insect pest damaging the rice crop up to 33 percent. Understanding genetic structure of the pest is essen More

  Rice is one of the most important and oldest crop and is one of the three leading crops in the world. Chilo suppressalis (Walker), rice striped stem borer, is a key insect pest damaging the rice crop up to 33 percent. Understanding genetic structure of the pest is essential for an effective pest management program and can corporate with control program. Aim of this stud was to determine the genetic structure of mitochondrial COII (mtDNA-COII) gene of the pest in north of Iran. Rice stem borer specimens were collected from rice fields in Guilan and Mazanderan provinces, at Caspian Sea coast at seasonal activity (spring) in 2016-2017. Genetic structure of 18 populations of the species was invesigated using polymerase chain reaction (PCR) followed by direct-sequencing of mtDNA-COII gene. The obtained sequences were compared with each other and the available data in GenBank database. In total, 312 and 676 specimens were collected from Guilan and Mazanderan provinces respectively. Results showed that all specimens from two provinces are identical are like specimen from South Korea with GenBank ID MK207057. Manuscript profile
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  5 - The Study of Molecular and Population Diversity of Rutilus frisiikutum in Anzali Lagoon and Shirud River by Using of Molecular Genetic Method
  فریدون چکمه دوز قاسمی شهرام بهمنش مهتاب یارمحمدی محمد حسن زاده صابر
  Anzali Lagoon and Shirud River are two important spawning areas in south Caspian Sea for kutum. Due to the economically valuable of this fish, molecular and population genetic structure of this fish was investigated using microsatellite markers.For this purpose, 100 sam More

  Anzali Lagoon and Shirud River are two important spawning areas in south Caspian Sea for kutum. Due to the economically valuable of this fish, molecular and population genetic structure of this fish was investigated using microsatellite markers.For this purpose, 100 samples from two regions were collected and DNA was extracted. Ten pairs microsatellite primer was used for PCR which all made polymorphic patterns. Data resulted: 191 alleles were observed. The maximum numbers of alleles (18) were found in two loci (Ca1 and Ca3) and the minimum number of alleles (2) was found in MFW1 locus. The differences between samples of two regions were not statistically significant (P>0.05) neither for average number of alleles per locus nor for observed heterozygosity. The calculated Fst was 0.056 and significant (P Manuscript profile