-
Open Access Article
1 - A Case Report of Mixed Infection in Holstein cattle with Theileria annulata and Babesia bigemina in one of the Tabriz Traditional dairies
امیررضا جوادی ممقانی عباس شهبازی اسماعیل فلاح مجید خانمحمدی احمد نعمت اللهی جواد اشرفی هلان مجید فرتاش وند محمود محامی اسکوئیBabesia bigemina and Theileria Anulata infection is common in tropical and sub tropical areasof Iran. Simultaneous infection with Theileria annulata and Anaplasma marginale in cattle hasbeen reported from Kerman, but there is not any published report on Simultaneous inf MoreBabesia bigemina and Theileria Anulata infection is common in tropical and sub tropical areasof Iran. Simultaneous infection with Theileria annulata and Anaplasma marginale in cattle hasbeen reported from Kerman, but there is not any published report on Simultaneous infection ofcattle with Theileria annulata and Babsia bigemina in Iran. The present report implies a case ofconcurrent infection of cattle with Theileria annulata and Babesia bigemina in one of thetraditional dairies of Tabriz. Clinical signs include swelling of the superficial lymph nodes,fever (40-41°C), anorexia, and increased hearing area of the heart sound, tachycardia, severeanemia, Haemoglobinuria, and Cachexia. Blood smears prepared from the ear vein and stainedwith Geimsa stain and observation of the morphologic characteristics by light microscope andresults of PCR method, showed simultaneous infection with Theileria annulata and Babsiabegemina. Manuscript profile -
Open Access Article
2 - Search of Tash-like nucleotide sequence(s) in Iranian ovine theileria genomes
حمیدرضا موسویان حمیدرضا حدادزاده پروانه خضرائی نیا بهرام کاظمی مژگان بنده پور مهدی نام آوری آمنه کوچکیTheileria lestoquardi and Theileria ovis are the causative agents of ovine theileriosis in Iran. Thedisease severity and the outcomes of these two species are completely different. Theilerialestoquardi unlike Theileria ovis, causes a severe disease with a high morbidity MoreTheileria lestoquardi and Theileria ovis are the causative agents of ovine theileriosis in Iran. Thedisease severity and the outcomes of these two species are completely different. Theilerialestoquardi unlike Theileria ovis, causes a severe disease with a high morbidity and mortalityrate called ovine and caprine malignant theileriosis. The pathogenicity aspects of T.lestoquardiare poorly understood. Many studies have investigated the pathogen molecules of Theileriaannulata and Theileria parva involved in both host cell transformation and proliferation. Tash(Theileria annulata schizont) pathogens proteins in theileria annulata are secrotome proteins,synthesized in schizont stage, located to parasite and host cell nucleus and are related to parasitepathogenicity. In the present study, multiple primers were designed based on Tash genes and thepresence of Tash-like sequence in Theileria lestoquardi and Theileria ovis genomes wereassessed. A 420 bp nucleotide sequene in T.Lestoquardi which was very similar to TashHN intheileria annulata was identified. This is the first report of a Theilerial lestoquardi sequence thatwould appear related to pathogenecity of malignant theileriosis in sheep. Manuscript profile -
Open Access Article
3 - Geographic Determination of Theileria ovis in goat in Tehran Province using molecular method
Khodaveisi, M., Rahbari, S., Shayan, P., Hoghooghi Rad, N. .Theileria ovis is one of the benign hemoprotozoal agents in sheep and goat in tropical and subtropical regions which is transmitted by ixodid ticks. This study was carried out on 400 randomly selected healthy goats for determining the distribution of T. ovis. After prep MoreTheileria ovis is one of the benign hemoprotozoal agents in sheep and goat in tropical and subtropical regions which is transmitted by ixodid ticks. This study was carried out on 400 randomly selected healthy goats for determining the distribution of T. ovis. After preparing the blood smears, they were examined staining by Giemsa method for microscopic studies. In order to molecular diagnosis, DNA was extracted from blood samples. Subsequently, the genomic DNA from piroplasms was first extracted and then amplified with the primer pair using hyper variable region V4 of 18S rRNA for simultaneous differentiation of Theileria and Babesia. Differential diagnosis of Theileria ovis from T. annulata and T. lestoquardi was performed using Semi-nested PCR technique on the PCR products with primers specific derived from hyper variable region V4 of 18S rRNA, P2, P3 for T. ovis and with primers specific derived from ms1-2 gene for T. lestoquardi (P4, P6) and T. annulata (P5, P6). The results of microscopic examinations showed that 1.7 % (7/400) were positive for the presence of T. ovis in comparison to T. ovis which were 6% (24/400) in Semi-nested PCR. The results of present study revealed that T. ovis is detectable in goats without any clinical manifestation. Therefore, the goats can be served as reservoirs of the infection for ticks and transmission agents of this parasite to the sheep. Manuscript profile -
Open Access Article
4 - Molecular detection of Theileria annulata and Theileria orientalis in cattle of Shahreza city, the south region of Isfahan province, Iran
, MR. Soltani , V. Noaman , Y. Pirali , HR Azizi,Theileria are important hemoprotozoan parasites of cattle, transmitted by ixodid ticks leading to diseases which range from mild in apparent reactions to highly fatal diseases. This study aimed to determine the variety of Theileria species among cattle of Shahreza city, MoreTheileria are important hemoprotozoan parasites of cattle, transmitted by ixodid ticks leading to diseases which range from mild in apparent reactions to highly fatal diseases. This study aimed to determine the variety of Theileria species among cattle of Shahreza city, the south part of Isfahan Province. A total of 253 blood samples were randomly collected via the ear vein and jugular vein from healthy cattle for microscopy and molecular examination, respectively. The extracted DNA from blood cells was amplified by Theileria-all primers, which amplify an approximately 400bp DNA fragment from the region of the 18S rRNA gene from various members of the genus Theileria. All cattle positive samples were further analysed for the presence of Theileria annulata and Theileria orientalis by specific semi-nested PCR. T. annulata and T. orientalis were identified by specific semi-nested PCR in 54.9% and 47% of cattle blood samples, respectively. Chi-square (x2) tests were used to compare molecular prevalence values relative to Season, Farm, Type, Hygiene, Vectors, Age, and Milk Yield. T. orientalis prevalence was significantly higher in cattle in summer as compared to those in other seasons (p < 0.05). The prevalence rates of T. orientalis in different seasons, hygiene levels, and vectors were significantly different (p < 0.05). T. orientalis prevalence was higher in spring, farms with tick vectors and normal level of Hygiene. The examination of 50 microscopic fields showed 14.39% sensitivity and 99.12% specificity compared to molecular examination. The Kappa coefficient between molecular and microscopy (50 fields) techniques indicated a poor level of agreement (Kappa= 0.124). This study is the first molecular detection of Theileria species from cattle in the south of Isfahan province, Iran. Manuscript profile -
Open Access Article
5 - Study the frequency of Theileria, Babesia and Annapalsma infections from cattle in Alborz province using microscopic examination and PCR
Mohammad Abdoli , L Abdolmohammadi KhiavAnaplasmosis, babesiosis and theileriosis is parasitic diseases that can cause significant damage to the livestock industry. Despite the importance of the pathogenicity of theileriosis, babesiosis and anaplasmosis in large animal populations, in Iran, studies have been MoreAnaplasmosis, babesiosis and theileriosis is parasitic diseases that can cause significant damage to the livestock industry. Despite the importance of the pathogenicity of theileriosis, babesiosis and anaplasmosis in large animal populations, in Iran, studies have been performed mainly on small ruminants. So, the aim of this study was to the diagnosis of the frequency of Theileria, Babesia and Annapalsma infections from cattle in Alborz province using microscopic examination and PCR (Polymerase Chain Reaction). For this purpose, out of 130 dairy cattle were taken blood samples and stained with Giemsa dye. Positive smear samples were incorporated for molecular detection. DNA was extracted from blood samples with phenol-chloroform and performed PCR using 18S rRNA, 16S rRNA and TamS primers to identify of the genus and species. The results of this study showed that frequency of piroplasmic form was observed in 9.23% of cases. The 18S rRNA PCR findings revealed 5.38%, were confirmed infection with Theileria and Babesia. Also, Tams1 PCR results were indicated Theileria anulata infection in all cases. 16S rRNA PCR was detected in 3.85% for Anaplasma. No case of Babesia was observed. Considering the cases of infection with T. anulata and Babesia and the importance of some species of Babesia, the importance of further studies to evaluate these diseases in order to improve the livestock industry seems necessary. Manuscript profile -
Open Access Article
6 - مطالعه وقوع آلودگی به گونههای مختلف تک یاخته تیلریا با روش Semi-Nested PCR در دامداریهای سنتی دو منطقه اکولوژیکی استان گلستان
پیمان قائمی ناصر حقوقی راد پرویز شایان بریگیته اکرت -
Open Access Article
7 - مقایسه حضور تک یاختههای خونی در گاوهای به ظاهر سالم مناطق مختلف جغرافیایی کشور با استفاده از روش PCR
بهاره چشتی غلام رضا رزمی ابوالقاسم نقیبی -
Open Access Article
8 - Detection of Theileria orientalis infection in indigenous buffaloes of West Azarbaijan province using molecular and microscopic tests
Babak Narimani Nasser Hoghooghirad Parviz Shayan Sadegh Rahbari Kasra Esmaeil-NiaThe causative agent of bovine theileriosis is obligatory intracellular protozoa called T. annulata which results in severe anemia, severe pulmonary edema and rapid death in non-native animals. The present study was aimed to detect the current state of Theileria infectio MoreThe causative agent of bovine theileriosis is obligatory intracellular protozoa called T. annulata which results in severe anemia, severe pulmonary edema and rapid death in non-native animals. The present study was aimed to detect the current state of Theileria infection in indigenous buffaloes and its association with environmental factors in four cities of West Azarbaijan province using microscopic tests (blood smear evaluation) and molecular assays. Blood samples were randomly collected from 291 buffaloes in the region, during March to July 2014. Direct and semi-nested PCR techniques were used to detect DNA of Theileria spp. using specific pair primer 18SrRNA. In the microscopic assay method, presence of this parasite in four (1.37%) buffaloes was approved. The molecular assays revealed that 4 out of 291 animals (1.37%) were infected by T. annulata. Also the present study showed that two female adult buffaloes (0.68%) were simultaneously infected by T. annulata and T. orientalis. Apparent and real prevalence of infection of buffaloes to T. annulata in the molecular method were 1.37 and 1 percent respectively. The chance of infection by T. annulata and T. orientalis were equal. Molecular prevalence of T. annulata in Urmia and Salmas were 2.2 and 2.3 percent respectively (p < /em><0.05). Molecular prevalence of T. orientalisin Urmia was 2.2%. This is the first study to indicate the presence of infection with T. orientalis in indigenous buffaloes of Urmia city in Iran. Manuscript profile -
Open Access Article
9 - Determination of infestation rate of Ixodidae ticks to Theileria annulata in Urmia region by polymerase chain reaction (PCR)
Ahmad Nematoallahi Iraj PanahiTheileriosis is one of the most important protozoan parasitic diseases in Iran that causes economic losses and mortality in livestock. The disease is transmitted by Ixodid ticks and is characterized by fever, anemia, anoxia and eventually death. Diagnosis of the disease MoreTheileriosis is one of the most important protozoan parasitic diseases in Iran that causes economic losses and mortality in livestock. The disease is transmitted by Ixodid ticks and is characterized by fever, anemia, anoxia and eventually death. Diagnosis of the disease was previously based on staining of blood and lymph node samples by Giemsa method. The use of molecular methods is preferred over previous methods due to their high sensitivity and specificity. Due to the fact that Ixodid ticks that can carry the disease are found in many traditional Iranian farms, it is very important to identify and introduce these ticks. The aim of this study was molecular identification of Theileria annulata infection in ticks isolated from cows in Urmia region. A total of 100 ticks from herds with a history of Theileria annulata infection in the area were collected and transferred to the laboratory. Ticks were identified using diagnostic keys. Salivary glands of ticks were separated and analyzed by PCR after isolation using Theileria annulata specific primer (N516, N517). Out of 100 ticks, ten genera and species including Hyalomma anatolicum anatolicum (33%), Hy. detritum (16%), Boophylus anulatus (16%), Dermacentor marginatum (14%), Rhipicephalus bursa (9%), Hy. anantolicum excavatum (7%), D. niveus (4%) and Haemaphysalis punctate (1%) were identified. The predominant tick was identified as Hyalomma anatolicum anatolicum. PCR test showed the presence of 721 bp specific band on agarose gel in 62% of tick samples. The high rate of infection in vector mites in the area indicates their high potential for infection of cows with the agent of Theileria annulata. Manuscript profile -
Open Access Article
10 - Detection of Theileriaovis in vector ticks by Polymerase Chain Reaction method (PCR) in Lorestan province
naser hoghoughirad saeed hashemi mohammad abdigoudarzi Theileriosis is caused by an intracellular protozoan that causes great economical losses to animal husbandry in different regions of Iran . The present study was done in order to identify of Theileriaovis in hard ticks in Lorestanprovince More Theileriosis is caused by an intracellular protozoan that causes great economical losses to animal husbandry in different regions of Iran . The present study was done in order to identify of Theileriaovis in hard ticks in Lorestanprovince . Amongst five different regions in Lorestan, and during the April-to-july period of the year 2012, 265 cases of hard ticks were collected from the ear and the body surface and 100 blood smears from ear veins of anemic and feverish sheep. DNA extraction was done from the salivary glands of collected ticks and PCR test was performed using a pair of 520 bp specific primer of SSurRNA gene of T. ovis . The microscopic examinations of blood smears showed that 12 samples of blood smears ( 12 %) contained the piroplasmic forms of Theileriaspcies. The PCR revealed that 37 out of 152 Rhipicephalussanguineus (24/34 % ) were positive for T. ovisgenum including 21 female (13/81%) and 16 male (10/52%) ticks while the other ticks were not infected by this parasite. Out of total of 265 tick samples, R.sanguineus was highest (38.35%) and Haemaphysalispunctatahad the lowest (3/01%) frequencies. Regarding the vast distribution of R.sanguineus in the area, it seems that this tick may be the main vector of T.ovis in Lorestan province, Iran. Manuscript profile -
Open Access Article
11 - Comparative study between microscopic and PCR analysis in a population of piroplasm carrier sheep in five region of khoramabad, Iran
Sh Yavari P. Shayan S. Bokai N. Amini niaIn the present study the rate of Theileria and Babesia spp. infection of sheep housing in Khoramabad, Iran was determined by using PCR technique and traditional giemsa staining method. DNA was extracted from blood samples that collected from 100 sheep from five regions MoreIn the present study the rate of Theileria and Babesia spp. infection of sheep housing in Khoramabad, Iran was determined by using PCR technique and traditional giemsa staining method. DNA was extracted from blood samples that collected from 100 sheep from five regions of Khoramabad and amplified by using specific primers derived from 18S rRNA gene of the mentioned piroplasms by PCR technique and subsequently analyzed on agarose gel using ethidium bromide under UV condition. Additionally, the prepared blood smears of each sheep were analyzed by Giemsa staining method. The results showed that structures assembling the mentioned piroplasms were detectable in 15 blood smears. Twelve of them were determined as Theileria spp. and the remaining three as Babesia spp. The PCR results showed that 28 samples were positive. The PCR product specific for Theileria spp. amplified in 22 samples (22%). Babesia spp. were detected in 6 blood samples (6%). The apparent agreement between two tests was 81%. When PCR technique is considered as golden standard method, the sensitivity and specificity of the other method were measured as 42% and 95.8%, respectively. Manuscript profile -
Open Access Article
12 - The Survey of Bovine Theileriosis in Borujerd Township
H. Vosughi R. GoodarziThis study was performed during the one year period, from March 21st 2011 to 2012, for detection v infection rate of Theileriosis in Borujerd township, and 860 cows were tested (220 samples in each season). First, the data related to every cow was recorded in a questio MoreThis study was performed during the one year period, from March 21st 2011 to 2012, for detection v infection rate of Theileriosis in Borujerd township, and 860 cows were tested (220 samples in each season). First, the data related to every cow was recorded in a questionnaire. Second, all cows were clinically examined and the positive cases which had Lymphadenitis, icterus in the vaginal mucosa and eye, fever, constipation, hyperpnea, diarrhea, coughing, watery eyes and mouth were distinguished and depleted from ear and forthwith were fixed by ethyl alcohol. Last, the blood smears samples were stained by Giemsa method and tested from view point of presence of piroplasmosis figures. To sum up, 536 of the 860 samples (62.32%) were positive and there were significant differences between male and female (P≤0.05). The most amount of infection was in spring (52.23%) and the least was in winter (0.93%) (P≤0.05).The most of infection rate was in over three years old cows (71.26%) and the least was in under one years old cows (3.54%) (P≤0.05). Manuscript profile -
Open Access Article
13 - Suspension culture of Neospora caninum using lymphocyte transformed by Theileria lestoquardi
Shamsi Ezhdahakosh pour Mohammad Mehdi Namavari Nahid Naghshgar Abdollah Rahimian Maryam Mansoorian Masoume HayatiBackground and Objectives: Neospora caninum is one of main etiologic factors of abortion in in cattle. The mass production of protozoa in laboratory conditions is performed based on growing in different cell lines. Since using suspension cell lines are very cost e MoreBackground and Objectives: Neospora caninum is one of main etiologic factors of abortion in in cattle. The mass production of protozoa in laboratory conditions is performed based on growing in different cell lines. Since using suspension cell lines are very cost effective for mass production of biological products, this study aimed to evaluate the suspension cell culturing for production of this protozoan. Materials and Methods: This study was experimentally performed bases on growth of N. caninum tachyzoites on Ka6 cell line (a cell line obtained from cattle infected with Theileria lestoquardi, Razi Institute, Shiraz, Iran. Next, based on MTT assay, ability of this cell culture for production of N. caninum was compared with Vero cell as the best current cell line for this purpose. Results: The results showed that N. caninum tachyzoites could enter into K6a cell lines and after replication released from the cells successfully. Replication of the tachyzoites was significant in both Vero and K6a cell lines in compare to the control. Conclusion: To our knowledge, this is the first and successful report of suspension culture of N. caninum tachyzoites. Although, the rate of N. caninum proliferation on Ka6 cell line did not show any significant difference in compare to Vero cell line, since Ka6 cell line is a transformed lymphocytic cell and it is possible to grow massively this cell line as suspension bioreactors, it is preferred to Vero cell line. Manuscript profile -
Open Access Article
14 - Isolation of Theileria lestoquardi Sporozoite in order to Produce a New Cell Line Buket
Nahid Naghshgar Mohammad Mehdi Namavari Shamsi Ezhdahakosh pour Mohammad Abdi Goodarzi Mohammad Hosein Hoseini Kasra Esmail nia Masoume Hayati Omid Reza Amr abadiIntroduction and Objectives: Malignant sheep and goats theileriosis is one of the most important protozoan diseases of sheeps in Iran. Theileria lestoquardi is the only intracellular eukaryotic pathogen that is able to transform its host cells reversibly. Therefore, des MoreIntroduction and Objectives: Malignant sheep and goats theileriosis is one of the most important protozoan diseases of sheeps in Iran. Theileria lestoquardi is the only intracellular eukaryotic pathogen that is able to transform its host cells reversibly. Therefore, design of a cell line of ovine lymphocyte that is transformed by the T. lestoquardi is very valuable. Materials and Methods: The breeding ticks was infected by feeding on the sheeps that were carrier of Theileria lestoquardi sporozoite in order to obtain homogeneous infected ticks. Then, ovine lymphocytes were infected with Theileria lestoquardi sporozoite stabilate to produce lymphocytic cell line containing T. lestoquardi. Result: This research resulted in the development of a new ovine lymphocytic cell line called as Booket. Molecular study confirmed that the cell lines were transformed by T. lestoquardi. The gene sequence of this isolate has been submitted to GenBank with accession no. GU233776. Conclusion: In this study the second lymphocytic cell line containing T. lestoquardi was established in Iran. Manuscript profile
Sanad
Sanad is a platform for managing Azad University publications
Links
Related Centers
Technical Support
Official pages
The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024