Background & Objectives: Toxoplasma gondii is a parasite which definitive hosts, Felid and cats, play a critical role in transmission and distribution of the parasite and their immunization is a proper way to control the infection. The aim of the present study is to More
Background & Objectives: Toxoplasma gondii is a parasite which definitive hosts, Felid and cats, play a critical role in transmission and distribution of the parasite and their immunization is a proper way to control the infection. The aim of the present study is to evaluate the immunogenicity of Toxoplasma gondii attenuated isolate produced in Razi Institute branch of Shiraz in cats
Materials & Methods: Twelve male DSH (Domestic short hair) kittens aged 3-6 months were randomly divided into three groups; each contains four cats. The First group received tachyzoites of Toxoplasma gondii attenuated isolate at a dose of 10×106 by injection, the second group received a dose of 25×106 orally and the last group received culture medium as a control. The humoral immune responses and Cellular immunity evaluated by modified agglutination and skin test. One month after immunization, all cats were challenged by Toxoplasma's PRU strain, after which the cats' feces were collected and molecular tests were performed for five days.
Results: The results of modified agglutination and skin test showed that administration of oral and injection vaccine had a significant increase in comparison with control. Also, Molecular testing after challenge revealed that immunization of cats by oral and injection way of Toxoplasma gondii attenuated isolate could successfully prohibit oocysts shedding in cats.
Conclusion: In order to the significant immune responses and prevention of oocysts shedding, it is suggested that, using this isolation of Toxoplasma gondii as a possible candidate vaccine for cats in future studies.
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The protozoan Neospora caninum is known worldwide as one of the most important causes of abortion storms in cattles, especially when dogs living in the cattle farms. Currently, the most consideration is on live attenuated vaccines. The main goal of this research is to e More
The protozoan Neospora caninum is known worldwide as one of the most important causes of abortion storms in cattles, especially when dogs living in the cattle farms. Currently, the most consideration is on live attenuated vaccines. The main goal of this research is to evaluated live-attenuated Neuspora caninum (NC-a) strain as an effective vaccine to prevent oocyst shedding in dogs. Sixteen dogs were randomly divided into four equal groups including live attenuated strain without adjuvant, acute strains, live attenuated strain with Montanide adjuvant and control group. All the groups were immunized twice, two weeks apart. One month after the second injection, all dogs were challenged orally by acute NC-1 strain. Immune responses were assay by agglutination test and molecular test was used to assess oocyte shedding. The results showed that the immunized group with live attenuated strain with adjuvant produces the highest immune response. Molecular test also showed that the immune response in immunized dogs prevented oocyst shedding after challenge and the best result is in the group of dogs immunized by live attenuated strain with adjuvant. Overall, the results of this study showed that research on the live attenuated Neospora caninum vaccine with oily adjuvants in dogs is secure and effective in creating an immune response and controlling oocyte shedding. In conclusion, this experimental vaccine can be used as a promosing vaccine in towards researches to obtain a succesful vaccine to control neosprosis in dogs.
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For the purpose of identifying the most probable type of yeast which manifests the milk claws,15 industrials farms, which held holstein cattle for milk production,were selected randomly.samples were taken from the internal surface of the liners by sterile swabs More
For the purpose of identifying the most probable type of yeast which manifests the milk claws,15 industrials farms, which held holstein cattle for milk production,were selected randomly.samples were taken from the internal surface of the liners by sterile swabs and then the samples were taken to the mycology laboratory.the samples were cultured in sabourauds dextrose agar and kept in room temperature.the final and definite recognition was done by the yeasts recognition kit(rapid yeast plus system).the type of yeasts which detected were include:C.albicansl C.krusei C.rugosal Cryptococcus.neoformanse,Cryptococcus.humiculus,Crytococcus.albicus,Rodotrola.rubra,Geotrichum.candidum
,Trichosporon.beigelly,Sporodiobolus.salmonicolor.in this study,relationship between number of milk claws washing per day and type of disinfected solution with detection of saprophyte yeasts were also considered.
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