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      • Open Access Article

        1 - Evaluatoin of the resistance of some citrus rootstocks to the citrus nematode, Tylenchulus semipenetrans, in Fars province, Iran
        Kavous Ayazpour Abdolhosein Aboutalebi Majid Pakniyat
        The citrus nematode, Tylenchulus semipenetrans, is widely distributed in the citrus orchards of Fars province, Iran. The nematode  causes slow decline and serious crop losses. Host resistance is the most effective method for controlling the disease. Seedlings of so More
        The citrus nematode, Tylenchulus semipenetrans, is widely distributed in the citrus orchards of Fars province, Iran. The nematode  causes slow decline and serious crop losses. Host resistance is the most effective method for controlling the disease. Seedlings of some rootstocks including Minneola (C. paradise × C. reticulata), Citrus ourantium, C. lmettioides, C. aurantifolia var. Ahvazi (a natural hybrid), C. maxima, Fortunella margarita, C. medica,  C. medica var. Otroj, C. lemonia var. Lisbon, C. taivanica, C. volkameriana, C. lemonia var. Eureka, C. aurantifolia, C. grandis, C. lemonia var. Rough Lemon, C. macrophylla (Alemow), Poncirus trifoliata, Swingle Citromelo(C. paradise × P. trifoliata) were evaluated for their resistance to the citrus nematode. The seedlings were planted in the pots and citrus orchard. In case of pot experiments, the three months old rootstock seedlings were transplanted to the pots with 40 J2/g of soil. The roots of seedlings were scored for nematode infestation after six months. For orchard experiments, the five months old rootstock seedlings were transplanted to naturally infested soil in a citrus orchard with 25 J2/g of soil. The roots of these seedlings were evaluated for nematode infestation 4.5 and 15 months after transplanting. The susceptibility of rootstocks to citrus nematode was evaluated on the base of the number of penetrated females per gram of feeder roots. In both experiments P. trifoliata, Swingle citromelo and Citrus ourantium were resistant to citrus nematode, whereas the other rootstocks supported a high population of the nematode.  Manuscript profile
      • Open Access Article

        2 - Differences pathogenicity of the fungus Cercospora beticola Sacc. caused cercospora leaf spot disease in sugarbeets and evaluation of sugarbeet cultivars resistance to the disease
        Yashar Riazi Mojdeh Maleki Dariush Shahriari
        Cercospora leaf spot disease caused by Cercospora beticola Sacc. is considered to be the most destructive foliar pathogen of sugarbeet which result in highest loss of yield and quality of sugarbeet in warm and humid environments. Control of these fungi often has been un More
        Cercospora leaf spot disease caused by Cercospora beticola Sacc. is considered to be the most destructive foliar pathogen of sugarbeet which result in highest loss of yield and quality of sugarbeet in warm and humid environments. Control of these fungi often has been unsuccessful by the use of pesticides application; hence, the best method for disease control is the production and implementation of resistant likely cultivars. For this purpose, fungal isolates collected from south and north areas of Iran, Purification and Pathogenicity of isolate the genotype (191), susceptible to disease with spore suspension was implemented on leaves in greenhouse Then for characterizing isolates virulence, like pathogenicity method was performed inoculation of the fungus C. beticola on susceptible (191). The disease severity index after the appearance of disease symptoms, 35 days after sporulating was done by KSW method. Cultivars response assessment, 30 cultivars of sugarbeet to leaf spots in Gharakheyl research station under field conditions was conducted on completely random block design with three replications. Statistics from the infection severity were taken in June and July with an interval of one month. Also for evaluation of genotypes resistance of detached leaf disks in laboratory conditions in sterile environment, 1.8 cm diameter discs were prepared from the leaves of mentioned genotypes and laid on water agar rectangular plates and with spore suspension were sporulated . A total of sampling were obtained 18 isolates of pure C. beticola from different areas that all of them were high virulence, Although in terms of the disease severity were different, but the differences placed in the quantity range between 77.7-98.9 percent. In response Study of 30 genotypes of sugarbeet to causal agent in field conditions, the best time recording of disease severity is done from Gharakhey Ghaemshahr in favorable climatic mid June to late July. In this study, only 5 genotype: 32333, 32335, 32295, 32304 and 32319 showed response moderately susceptible "MS" and the rest of the group were susceptible "S" or very susceptible "VS". It was also determined a high correlation (91%) between assessment of detached leaf disks resistance in laboratory and field conditions as well as these two methods can replace each other Manuscript profile
      • Open Access Article

        3 - Identification of Pathotypes in Ascochyta rabiei (Pass.) Labr., the agent of Ascochyta blight in chickpea in provinces of Kermanshah and Ilam and resistance evaluation of chickpea cultivars and lines against disease
        Shahrzad Khalatbari Dariush Shahriari Mojdeh Maleki
        Ascochyta blight caused by Ascochyta rabiei Pass. (Labr.)is one of the greatest disease in chickpeathat cause total yield loss if the environmental conditions are favorable. The use of resistant cultivars is the most effective management strategy for ascochyta blight in More
        Ascochyta blight caused by Ascochyta rabiei Pass. (Labr.)is one of the greatest disease in chickpeathat cause total yield loss if the environmental conditions are favorable. The use of resistant cultivars is the most effective management strategy for ascochyta blight in chickpea. In our study, 24 different isolates of A. rabiei from plants infected by this disease were obtained in provinces of Kermanshah and Ilam and then isolated and purified. For evaluation of the morphological characteristics, discs of colonies of fungal isolates were sown in PDA, CDA, CSA and WA media. For pathotypes identification, the isolates were sporulated on differential chickpea cultivars by concentrations with 106 spores per ml. 41 genotypes and chickpea cultivars inoculated by three A. rabiei pathotype like pathogenicity method in greenhouse. The severity of the disease index is noted from 1 to 9, according to the scale of Jan and Wiese and 12 days later when the intensity of symptoms reached to 90 percent on susceptible cultivar. The results of our study revealed morphological and genetic diversity of the fungus. In this survey, three pathotypes identified in different districts of Kermanshah and Ilam, respectively. Pathotypes 1 of 13 of total isolates was the most widely distributed then, pathotype II with 6 isolates and pathotype III with 5 isolates were the least ones. The response of 41 cultivars against three pathotype of A. rabiei, Adel, Azad cultivar that have just been released by the Agricultural Research Institute of Dryland over the past few years together with advanced line of Flip 03-109C against the pathotype III showed moderately resistant response while other lines and cultivars were susceptible or highly susceptible. In total, lines of Flip51-87C, Flip03-119C, Flip04-13C and Flip04-10C showed resistant or moderately resistant response for pathotypes. Manuscript profile