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      • Open Access Article

        1 - Ovicidal, larvicidal and oviposition deterrency effects of essential oil from Thymus vulgaris L. (Lamiaceae) on Callosobruchus maculatus (F.) (Col., Bruchidae)
        E. Dezfouli S. Moharramipour Sh. Goldasteh
        Extensive uses of synthetic pesticides caused development of resistant strains of insects to     pesticides and also environmental pollution. Therefore, the use of essential oils extracted from plants as pest control agents of stored product insects More
        Extensive uses of synthetic pesticides caused development of resistant strains of insects to     pesticides and also environmental pollution. Therefore, the use of essential oils extracted from plants as pest control agents of stored product insects has been received much attention in recent years. Essential oils contain various effects such as toxicity, repellency, oviposition and feeding deterrency on different species of insects. In this research, effects of essential oil of                    Thymus vulgaris L. (Lamiaceae) were studied on Callosobruchus maculatus (F.) (Col., Bruchidae) at 27±1º C and 65±5 % RH in dark condition. The essential oil was obtained via hydro distillation. Ovicidal and larvicidal effects of the essential oil were investigated on C. maculatus Results showed that old eggs were more sensitive than the young ones. However old larvae were less       sensitive than the young ones. The highest oviposition deterrency of C. maculates was observed at highest concentration of 1500 ppm. Manuscript profile
      • Open Access Article

        2 - Effect of thyme (Thymus vulgaria) extract on chemical, microbiological and sensory properties of sausage during storage
        کلارا Pirouti افشین Javadi فریبرز Nahidi
           Due to the side effects of synthetic food preservatives on human health, more studies have been recently focused on natural preservatives. This research aimed to investigate the effect of Thymus vulgaria extract on some of chemical (TVb-N), microbiological More
           Due to the side effects of synthetic food preservatives on human health, more studies have been recently focused on natural preservatives. This research aimed to investigate the effect of Thymus vulgaria extract on some of chemical (TVb-N), microbiological (total count and psychrophilic count) and sensory (texture, taste, flavor and color) properties of susage during storage. Different concentrations (0, 1%, 2% and 3%) of thyme extract were added to the sausage farsh. After the production of sausages, the samples were stored in the refrigerator. During the storage period (0, 14, 28 and 42 days) the samples were subjected to chemical, microbialogical and sensory examinations. Results of total microbial count and psychrophilic count revealed a significant (p < 0.05) difference between the control group and the samples containg 2% thyme extract. Moreover, TVB-N and peroxide indices were highest among control sample which indicates the significance (p < 0.05) impact of thyme extract on the shelf-life of sausage samples. However, no significant (p > 0.05) difference was observed among treatment groups (1, 2 and 3% thyme). Regarding the results of sensory assays, no significant difference was observed between the samples containing 1% thyme extract and control group. On the other hand, the difference between the samples containing 1% and 3% thyme extract was not statistically significant (p < 0.05). Considering all above it was concluded that thyme extract at the concentration of 2% could be used as a preservative in sausage.  Manuscript profile
      • Open Access Article

        3 - Changes in growth characteristics and secondary metabolites in Thymus vulgaris L. under moderate salinity and drought shocks
        Sedegeh Fabriki ourang Behnam davoodnia
        In order to evaluation of  phytochemical and growth changes from Thymus vulgaris L. under moderate abiotic stresses, an experiment was carried out at Imam Khomeini International University in a completely randomized design with three replications during 2016. The t More
        In order to evaluation of  phytochemical and growth changes from Thymus vulgaris L. under moderate abiotic stresses, an experiment was carried out at Imam Khomeini International University in a completely randomized design with three replications during 2016. The treatments consisted of drought (50% FC), salinity (100 mM NaCl) and non-stress control (100% FC). Root fresh weight, root dry weight, root length, main branch diameter, sub-branch diameter, sub-branch number, shoot dry weight, shoot fresh weight, plant height, relative water content and chlorophyll content were measured. Also, total phenols, flavonoids and anthocyanins were assayed in leaves, roots and stems by using spectrophotometer. Statistical analysis showed that drought and salinity had significant effects on morphological traits, except for shoot dry weight and number of lateral branches. Generally, morphological traits affected by drought stress in compared with non-stress condition; so that, plant height, main branch diameter, lateral branch diameter, root dry weight, root fresh weight and shoot fresh weight decreased under drought stress compared to non-stressed plants. Analysis of variance showed that stresses had significant effects on leaf and stem flavonoids and phenol contents. By contrast, there was no significantly difference between drought and saline stresses in terms of total flavonoids and phenol of roots. Mean comparisons showed that the amount of leaf phenols and flavonoids increased under drought stress in compared with non-stress condition. In conclusion, this study showed that drought and salinity were increased secondary metabolites in thyme, but the effect of salinity in increasing of secondary metabolites was lower than drought stress.   Manuscript profile
      • Open Access Article

        4 - Effect of gamma ray irradiation on the morphological and phytochemical characteristics of Thymus vulgaris L.
        Fahimeh Salehi Azizollah Kheiri Ali Eskandari Mohammadbagher Rezaeei
      • Open Access Article

        5 - The effect of organic and chemical fertilizers on quantitative and qualitative characters of essential oil in Thyme (Thymus vulgaris L.)
        Mehrab Yadegari
        Organic manuares have important effectiveness on quantity and quality of medicinal plants and this plants have important role in food industries, then this research was done to evaluate the effects of organic and chemical fertilizers on essential oil content and composi More
        Organic manuares have important effectiveness on quantity and quality of medicinal plants and this plants have important role in food industries, then this research was done to evaluate the effects of organic and chemical fertilizers on essential oil content and composition of Thyme. This research was conducted in a randomized complete block design with 4 replications in Research Farm of Islamic Azad University Branch of Shahrekord at 2020 and 2021. Treatments were: control (no fertilizer), cow manure, sheep manure, hen manure, horse manure, vermi-compost and complete chemical fertilizers. Each of animal manuares and vermi-compost in 20 t.ha-1 in planting time and complete chemical fertilizer in 1.5 g.l-1 concentration after V10 stage in two step by 3 weeks interval, were used. Shoot of plant samples beginning of flowering stage in end of vegetative stage (252-270 leaf) prepared from treatments separately in the end of august. For measurement of the content and yield of the volatile oil, 1000g of the fresh shoots harvested and mixed with water and placed in a flask connected to the condenser of a Clevenger apparatus and the GC analysis was done to detect the essential oil composition. The most weight of dry matter (352±17.1 g.m-2 in first year and 359.04±18.9 g.m-2 in second year) and essential oil content (0.91±0.1% in first year and 1.1±0.01% in second year) were observed in chemical fertilizer was the same group with vermi-compost and hen manure. Four components of P-cymene, Gamma terpinen, Carvacrol and Thymyl acetate are classified as cyclic monoterpene made more than 70% of essential oil content in different treatments. 29 components in essential oil of treatments were detected. The greatest amount of major components were Carvacrol (35.2-36.8%) in chemical fertilizer and vermicompost, P-cymene (8.3-7.8%) in chemical fertilizer; Gamma terpinene (18.6-18.7%) in vermi-compost and Thymyl acetate (4.6-4.8%) in hen manure. Manuscript profile
      • Open Access Article

        6 - Study the antimicrobial effects of ethanolic extract of Thymus vulgaris on foodborne bacteria in vitro
        Meysam Alimoradi Mehrdad Ataie Kachoie Fariborz Moattar
        Thymus vulgaris is a plant of family Lamiaceae that has high antimicrobial properties. The present study was performed to evaluate the antimicrobial effects of the ethanolic extract of Thymus vulgaris on food-borne bacteria. Aerial part of Thymus vulgaris was used to pr More
        Thymus vulgaris is a plant of family Lamiaceae that has high antimicrobial properties. The present study was performed to evaluate the antimicrobial effects of the ethanolic extract of Thymus vulgaris on food-borne bacteria. Aerial part of Thymus vulgaris was used to prepare methanolic extract. The diameter of the growth inhibition zone of bacteria was assessed using disk diffusion. Minimum inhibitory concentration (MIC) and Minimum Bacterial Concentration (MBC) of ethanolic extract of Thymus vulgaris were evaluated on the target bacteria using ELISA plate. The diameter of the growth inhibition zone of bacteria against the ethanolic extract of Thymus vulgaris ranged from 7.87±0.61 to 16.99±0.37 mm. Application of 50 mg/ml concentration of ethanolic extract of Thymus vulgaris caused the highest diameter of the growth inhibition zone of Pseudomonas aeruginosa (11.38±0.43 mm), Escherichia coli (12.00±0.35 mm), Staphylococcus aureus (16.33±0.69 mm) and Listeria monocytogenes (16.99±0.37 mm). The antimicrobial effects of ethanolic extract of Thymus vulgaris were dose-dependent (P <0.05). The diameter of growth inhibition zone of 50 mg/ml of ethanolic extract of Thymus vulgaris on Pseudomonas aeruginosa, Staphylococcus aureus and Listeria monocytogenes was higher than all antibiotics (P <0.05). The lowest MIC and MBC values were obtained for Staphylococcus aureus (4 and 6 mg/ml, respectively) and Listeria monocytogenes (4 and 6 mg/ml, respectively). Thymus vulgaris ethanolic extract can be used as a combination with antimicrobial properties in food. Manuscript profile
      • Open Access Article

        7 - تعیین بهترین زمان برداشت برای دستیابی به بیشترین بازدهی اسانس و تیمول در آویشن باغی Thymus vulgaris L در شرایط اصفهان
        امین هادی پناه احمدرضا گل پرور عبدالله قاسمی پیربلوطی حسین زینلی
        مقدمه و هدف: آویشن باغی با نام علمی (‏L.‎‏ ‏Thymus vulgaris‏) متعلق به خانواده  نعناعیان (‏Lamiaceae‏) ‏می باشد. آویشن یکی از مهمترین گیاهان دارویی مورد استفاده در صنایع داروسازی، غذایی، ‏آرایشی و بهداشتی بیشتر کشورهای توسعه یافت More
        مقدمه و هدف: آویشن باغی با نام علمی (‏L.‎‏ ‏Thymus vulgaris‏) متعلق به خانواده  نعناعیان (‏Lamiaceae‏) ‏می باشد. آویشن یکی از مهمترین گیاهان دارویی مورد استفاده در صنایع داروسازی، غذایی، ‏آرایشی و بهداشتی بیشتر کشورهای توسعه یافته است. آویشن یک ضدعفونی کننده قوی با ‏خاصیت آنتی اکسیدانی مناسب است. تحقیق حاضر با هدف تعیین بهترین زمان برداشت بر عملکرد تر ‏و خشک اندام‌های هوائی، درصد و عملکرد اسانس، درصد و عملکرد تیمول در گیاه دارویی ‏آویشن باغی به مرحله اجرا درآمد.‏روش تحقیق: به منظور تعیین بهترین زمان برداشت روی صفات مورد نظر آویشن باغی آزمایشی در قالب طرح بلوک­های کامل تصادفی با سه تکرار در مزرعه دانشگاه آزاد اسلامی واحد خوراسگان (اصفهان) در سال 1388 و 1389 انجام شد. تیمارهای مورد بررسی، دوره‌ های فنولوژیکی (زمان­های برداشت) بود که عبارت از دوره رویشی، ظهور آغازه‌های گل، ظهور50 درصد گل ‌آذین‌ها، گلدهی کامل و زمان تشکیل بذر بودند. میزان تیمول اسانس به وسیله دستگاه کروماتوگرافی گازی متصل به طیف سنج جرمی (GC/MS) مورد تجزیه و شناسایی قرار گرفت.نتایج و بحث: در این آزمایش مشخص شد که تأثیر دوره های فنولوژیک بر بازده و عملکرد اسانس، درصد و عملکرد تیمول گیاه آویشن باغی در سطح یک درصد معنی دار بود. مقایسه میانگین تیمارها نشان داد که بیشترین بازده اسانس مربوط به آغاز گلدهی بود که معادل 42/2% شد و نسبت به سایرین اختلاف معنی دار داشت. تجزیه و شناسایی ترکیب­های تشکیل دهنده اسانس­ها نشان داد که در تمام مراحل برداشت تیمول ترکیب اصلی اسانس بود. توصیه کاربردی/صنعتی: به طور کلی با توجه به نتایج این تحقیق، می توان مرحله 50% گلدهی را به عنوان مناسب ترین زمان برداشت برای عملکرد اسانس و تیمول آویشن باغی معرفی نمود. Manuscript profile
      • Open Access Article

        8 - Effect of pepper mint and thymus vulagaris extracts with and without flaxseed on eggs quality of layhng hens.
        Faramarz Keshavarz Moetamedi Reza Taherkhani Timur TanhaGhezli
        In order to survey the effects of extracts of garden thyme and pepper mint and their mixed extracts on yield quality characters of egg, an experiment were done in 2016 in the region of varamin on 80 old laying lineage hen lineage of lohmen light (LSL) in age of 60 to 68 More
        In order to survey the effects of extracts of garden thyme and pepper mint and their mixed extracts on yield quality characters of egg, an experiment were done in 2016 in the region of varamin on 80 old laying lineage hen lineage of lohmen light (LSL) in age of 60 to 68 week as a completely randomized block design with four treatment and five replication. Treatments were consists of control, extracts of pepper mint (0.5 percent), garden thyme (0.5 percent), pepper mint + garden thyme (0.25 % + 0.25%). All extracts of medicinal were used in the form of water dilution. Results of this experiment showed that using simultaneous of pepper mint extracts and garden thyme had significant effect on increasing yolk weight. Using each of medicinal plant extracts by- self had no significant effect on above parameters (p<0.01). Using extracts of garden thyme caused significant effects on Hough unit, cholesterol and density of yolk but had on significant effect on thickness of egg shell. Although mixed application of pepper mint and garden thyme caused increasing of shell thickness but this results were not significant (p<0.01). Manuscript profile
      • Open Access Article

        9 - Effect of Pepper mint and Thymus vulgaris extracts with and without flaxseed on performance of laying hens.
        Faramarz Keshavarz moetamedi Reza Taherkhani Timur Tanhaghezeli
        In order to survey the effects of extracts of garden thyme and pepper mint and their mixed extracts on yield and quantity characters of egg, an experiment were done in 2016 in the region of varamin on 80 old laying lineage hen lineage of lohmen light( LSL) in age of 60 More
        In order to survey the effects of extracts of garden thyme and pepper mint and their mixed extracts on yield and quantity characters of egg, an experiment were done in 2016 in the region of varamin on 80 old laying lineage hen lineage of lohmen light( LSL) in age of 60 to 68 week as a completely randomized block design with four treatment and five replication. Treatments were consists of control, extracts of pepper mint (0.5 percent), garden thyme (0.5 percent), pepper mint + garden thyme (0.25 % +0. 25%). All extracts of medicinal were used in the form of water dilution.Results of this experiment showed that using simultaneous of pepper mint extracts and garden thyme had significant effect on increasing egg weight, and final percent of yield, egg mass, and decreased feed conversion ratio. Using each of medicinal plant extracts by- self had no significant effect on above parameters (p<0.01). Manuscript profile