The effect of combining mord (Myrtus communis) leaf extract and olive oil (Olea europaea) in comparison with 1% silver sulfadiazine cream on skin wound healing in Balb/c mice

Subject Areas : biology

Farkhondeh Nemati ¹ , Ramin Ataee ² , Fatemeh Gorji ³ , Seyed Taleb Houseini ⁴ , Amin Lotfvarzi ⁵ , Ali Bagheri hashem abad ⁶

1 - Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Mazandaran,Iran
2 - Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran Medical Sciences, Sari, Iran
3 - Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Mazandaran,Iran
4 - Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Mazandaran,Iran
5 - Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Mazandaran,Iran
6 - Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Mazandaran,Iran

Received: 2022-04-21 Accepted : 2022-06-19 Published : 2022-02-20

Keywords: Wound healing, Olive oil, mord, silver sulfadiazine cream 1%,

Abstract :

Mord (Myrtus communis) is a plant with antibacterial and anti-inflammatory properties. Ointment of ethanolic extract of the leaves of the mord was prepared in olive oil in a ratio of 1: 1. Wounds developed in the full thickness of the skin in the dorsal region of the mice. The mice were then divided into control and experimental groups. The control group did not receive any treatment and the experimental group, which included the group receiving the combination of mord leaf extract and olive oil and the group receiving 1% silver sulfadiazine cream, were treated once a day. Finally, the percentage of recovery, average skin thickness, diameter of hair follicles, collagen formation, and angiogenesis were examined on the fifth, tenth, and fifteenth days. Data were analyzed using a one-way ANOVA test using SPSS software version 22. The results showed that the treatment with the combination of ethanolic extract of mord leaf and olive oil led to a significant increase in wound healing percentage, skin thickness, hair follicle diameter, compared to 1% silver sulfadiazine cream. There was also an increase in collagen formation and angiogenesis. These results have been a reason for accelerating the wound healing process in the samples treated with a combination of mord extract and olive oil. The results of this study showed that the use of mord leaf extract and olive oil accelerates the transition from the inflammatory stage to the stage of fibroblast hyperplasia due to the increase in collagen synthesis and blood vessels in this stage.