Investigation of association between rs11639084 and rs4774388 polymorphisms of RORA gene and Behçet’s disease in Iranian population

Subject Areas : biology

Parisa Zaree ¹ , Shohreh Zare karizi ² , Maryam Eidi ³

1 - Department of Biology, Pishva Unit, Islamic Azad University, Varamin, Iran.
2 - Department of Biology, Pishva Unit, Islamic Azad University, Varamin, Iran.
3 - Department of Biology, Pishva Unit, Islamic Azad University, Varamin, Iran.

Received: 2021-09-17 Accepted : 2021-09-17 Published : 2021-02-19

Keywords: RORA gene, 4ARMS-PCR, Behcet disease, autoimmune diseas,

Abstract :

Behcet's disease is a chronic autoimmune disease that can affect many body systems. Various reasons have been known for Behcet's disease, including genetic and environmental factors. However, approximately of Behcet disease have still unknown causes. Gene polymorphisms may effect on the incidence of Behcet disease. present study was aimed to the association of two SNPs in RORA genes in patients with Behcet disease.In this study, 100 people with Behcet disease were selected as patients Also 100 normal people were chosen as controls. Genomic DNA was extracted . The polymorphism of rs11639084 and rs4774388 of RORA gene were studied by using 4ARMS-PCR technique. Differences between allele and genotype frequency in two grouped were calculated by chi square test with p value>0.05. Logistic regression test were used for estimating odd ration and 95% CI by using SPSS ver.16 software.The frequency of three genotypes of CC, CT, and TT polymorphism rs11639084 in the patient group was 67, 30 and 3, respectively, and in the control group, 61, 28 and 11, respectively. The frequency of three genotypes of CC, CT and TT polymorphism rs4774388 in the patient group was 7, 40 and 53 and in the control group 21, 60 and 19. Finally, statistical analysis showed that there is a significant difference between the allele frequency of rs11639084 and rs4774388001 in patient samples and control samples.It was concluded that there is a significant relationship between some polymorphisms in RORA gene and Behcet's disease, which requires further studies in this regard.