Evaluation of CCND1 and ITGB1 genes expression in patients with breast cancer

Subject Areas : biology

Farnam Gholipour Maralan ¹ (Department of Biology, Pishva Unit, Islamic Azad University, Varamin, Iran.)
shohreh Zare Karizi ² (Department of Biology, Pishva Unit, Islamic Azad University, Varamin, Iran.)
Mohammad Ebrahim zadeh ³ (Department of Biology, Pishva Unit, Islamic Azad University, Varamin, Iran.)

Received: 2021-09-27 Accepted : 2021-10-21 Published : 2021-05-22

Keywords: breast cancer, Gene expression, CCND1, ITGB1, quantitative real-time PCR method,

Abstract :

Breast cancer is the most common type of invasive cancer and the second cause of cancer-related death in women. Therefore, evaluating the molecular mechanisms is of crucial importance in this respect. the CCND1 gene encodes the Cyclin D1 protein and Cyclin D1 overexpression has been shown to correlate with early cancer onset and tumor progression. Cyclin D1 overexpression can also down-regulate Fas expression, leading to increased chemotherapeutic resistance and protection from apoptosis. In addition, Integrin beta-1 (ITGB1), is a cell surface receptor that in humans is encoded by the ITGB1 gene. Abnormal levels of integrin beta-1D have been found in limb girdle muscular dystrophy and polyneuropathy.Sample from tissues of 30 patients with breast cancer and the normal margin of tissues were bought from Imam Khomeini hospital. Afterwards, genomic RNA extraction and cDNA synthesis using quantitative real-time PCR method was implemented and after primer design for CCND1 and ITGB1 genes, gene expression was analyzed. The CCND1 and ITGB1 genes demonstrated a significant expression increase in breast cancer tumor samples compared to adjacent normal samples (P <0.0001). The increased expression of CCND1 and ITGB1 genes may enhance the process of cell cycle and this condition can develop tumorigenesis; however more research is needed to confirm these findings.