Clinical and microscopic study of the effect of different concentrations of cardamom extract (Elettaria cardamomum) on the healing of skin wounds in diabetic rabbits with alloxan

Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)

Fatemeh Tavakoli Rad ¹ , loghman Akradi ² , Mahdieh Raeeszadeh ³

1 - دانش آموخته دکتری Graduated of veterinary medicine, Sanandaj Branch, Islamic Azad University , Sanandaj, Iranدامپزشکی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران
2 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Sanandaj Branch, Iran
3 - Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

Received: 2021-09-21 Accepted : 2021-11-30 Published : 2021-11-22

Keywords: Rabbit, Wound healing, Alloxan, Cardamom extract,

Abstract :

In this study, 30 male rabbits weighing 900 - 1000 g were randomly divided into 6 groups (positive control, negative control and test). After general anesthesia using a combination injection of ketamine 50 mg / kg and xylazine 5 mg / kg intraperitoneally to create ulcers in the rabbit skin a 10 mm circular punch was made. The wounds was measured with the aid of millimeter papers on the first, third, sixth, ninth, twelfth, fifteenth, eighteenth, and twenty-first days after surgery. This measurement is made at a specific time of day by a specific person. Complete recovery time was measured in negative, positive control and test samples. Then, on day 21, the wound was sampled. The specimens were subjected to histopathological studies after fixation of dewatering and staining.According to the results, there was no significant difference between control group and T1 group (P <0.05), but there was a significant difference between control group and T2, T3, T4, T5 group (P <0.05). The highest difference was between T5 and control group. It can be said that methanolic extract of cardamom at a dose of 400 mg / kg topical and feeding on cardamom powder pellets was more effective than all groups. Also, migration of epithelial tissue and reduction of wound openings in group T5 on days 15 to 21 were higher than other groups. Also, cardamom powder as the last plate of food significantly decreased glucose in diabetic groups (P <0.05). Cardamom lowers blood sugar by antioxidants such as flanoids. According to the results of this study, cardamom extract has a high ability to heal full-thickness wounds compared to chemical drugs.

References:

 

 

Arianfar,a. Sardarodiyan,m.  (2018) Investigation of the synergistic and antagonistic properties of green tea and Elettaria cardamomum extracts . Food Science and Technology, Volume:14 Issue: 11,25 - 36

Choucair, M. M., & Phillips, T. J. (1997). What is new in clinical research in wound healing. Dermatologic clinics, 15(1), 45-58.‏

Davari, S.A. Hajinezhad, M.R. Samadi, Eftekhari,  K.S. (2018) The Effect of Calendula, Aloe and Caster on Cutaneous Wounds Healing Process in Mature Male Rat. Journal of Rafsanjan University Of Medical Sciences, Volume:17 Issue: 2,93 - 104

Devi, P. S., & Shyamala, D. C. (1999). Protective effect of quercetin in cisplatin-induced cell injury in the rat kidney. Indian journal of pharmacology, 31(6), 422.‏

Duke JA. (1985), CRC handbook of medicinal herbs. Boca Raton: CRC Press: 274-6

Ghane, Z. Vazini, H. Pirestani, M. (2016) Protective Effect of Hydroalcoholic Extract of Elettaria cardamomum L. Fruits on Serum Levels of Liver Enzymes and Morphpological Changes in Lead Induced Male Rats . Journal of Mazandaran University of Medical Sciences, Volume:26 Issue: 142, 1 - 13

Hemmati, A. A., Arzi, A., & Amin, M. (2002). Effect of Achillea millefolium extract in wound healing of rabbit. Journal of natural remedies, 2(2), 164-167.‏

Hojjati, H . Kazemi, K. Tanideh, N. Sivani, E. Roshan, N. (2003) Evaluation of the effect of ointment obtained from the combination of egg yolk and tragacanth gum in wound healing in animal model. Journal of Medical Research, 2 , 33-39

Kwon, M. J., Song, Y. S., Choi, M. S., Park, S. J., Jeong, K. S., & Song, Y. O. (2003). Cholesteryl ester transfer protein activity and atherogenic parameters in rabbits supplemented with cholesterol and garlic powder. Life sciences, 72(26), 2953-2964.‏

 

Malekmohammadi, M. Tehrani A.H. Aghdami Nasser (2012) Skin structure and wound healing phases . Journal of Dermatology and Cosmetics, Volume:2 Issue: 4, 229

Mehvish, S. & Barkat, M. Q. (2018). Phytochemical And Antioxidant Screening Of Amomum Subulatum, Elettaria Cardamomum, Emblica Officinalis, Rosa Damascene, Santalum Album And Valeriana Officinalis And Their Effect On Stomach, Liver And Heart. Matrix Science Medica (MSM), 2(2), 28-33.‏

Moghbel, A., Hemmati, A. A., Agheli, H., Amraee, K. H., & Rashidi, I. (2005). The effect of tragacanth mucilage on the healing of full-thickness wound in rabbit.‏ Archives of Iranian Medicine, Volume:8 Issue: 4, 257 -262

Miravliaei, M. Arefiar, R. Talebi, A. Amin Jafari, A. Zahmatkesh, M. (2018) Histopathological examination of deep skin wound healing with hydroalcoholic extract of Mamitha (Glaucium corniculatum curt). Journal of Molecular Cell Research (Iranian Biology), 31 No. 1, 1-15

Mirazi, N. Taheri, S. (2016) Study of Elettaria cardamomum L. fruit's hydroethanolic extract on thyroids hormones serum level in hypothyroid male mice. Armaghane-danesh, Volume:21 Issue: 1, 27 - 39

Nabiuni, M. Azarnia, M. , Mousavi, R. Ramezani, T. (2014) The effect of Myrtus Communism leave extract cream on wound healing process in Wistar rats. Complementary Medicine Journal, Volume:4 Issue: 3, 854 - 864

Park, J. K., Lee, S. O., Cui, W. S., Kim, S. Z., Koh, G. Y., & Cho, K. W. (2005). Activity of angiotensin peptides in clitoral cavernosum of alloxan induced diabetic rabbit. European urology, 48(6), 1042-1050.‏

Patiño, O., Grana, D., Bolgiani, A., Prezzavento, G., Miño, J., Merlo, A., & Benaim, F. (1996). Pulsed electromagnetic fields in experimental cutaneous wound healing in rats. The Journal of burn care & rehabilitation, 17(6), 528-531.‏

Poozesh, R. Vaezi, Gh. H. Khajeh Dalavi, Gh. (2012) Comparison of the effect of intraventricular injection of aqueous and methanolic extracts of cardamom seed on PTZ-induced fear behavior in adult male rats. Journal of Animal Biology, Volume:4 Issue: 2, 13-21

Rezaei, E. Vazini, H. Pirastani, M. (2016) The effect of hydroethanolic extract of Elettaria cardamomum L. on serum levels of gonadotropins and testosterone in adult Wistar rats induced with lead acetate. Journal of Jorjani Biomedicine, 4 No. 2.20-32