Nephrotoxic effects of nanosilver in broiler chickens
Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)
Keywords: broilers, kidney, Histopathology, Silver, Uric acid,
Abstract :
Nanoparticle of Silver, with creating free radicals, can destroy many bacteria, viruses and fungi; therefore they have been recommended as a disinfectant in livestock’s production system. In this study 240 Ross 308 strain chicks in a completely randomized design (CRD) in four treatments at 0(control), 4, 8 and 12 ppm levels of silver nanoparticles in drinking water with four replicates within 16 separate floor pens and 15 birds in each pen were reared. At the end of growing period from each pen two broilers with the average weight were selected and after taking blood sample, sacrificed and their kidney were examined with light microscope by H&E staining. Histopathology showed dose depended changes such as congestion, presence of inflammatory cells including heterophile and lymphocytes, necrosis in tubular cells, proliferative glomerulonephritis and fibrosis. Lesions in the treatments 3(8ppm) and 4(12ppm) were more sever than the group 2(4ppm). Also, increased serum uricacid in treatment 4 (12ppm) compared to control group was significant (p <0.05). It can be concluded that using of nanosilver higher than 8ppm in drinking water can induce sever lesions in kidney of chickens.
1- نبینژاد، ع. عسکرانی، ن. (1387): کاربرد نانوسیلور در پرورش مرغ گوشتی، امیدها و نگرانیها. مجموعه مقالات پانزدهمین کنگره دامپزشکی ایران
2- نظیفی س. (1376): هماتولوژی و بیوشیمی بالینی پرندگان. انتشارات دانشگاه شیراز.چاپ اول. صفحه206-209
3- Ahari, H., Dastmalchi, F., Ghezelloo, Y., Paykan, R., Fotovat, M., Rahmannya, J., (2008):The application of silver nano-particles to the reduction of bacterial contamination in poultry and animal production. Food Manufacturing Efficiency. 2, 1: 49-53
4- Braydich-Stolle, L., Hussain, S., Schlager, J., Hofmann, M.C., (2005): In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicology Sciences. 88:412-419
5- Chang, A.L., Khosravi, V., Egbert, B., (2006): A case of argyria after colloidal silver ingestion. Journal of Cutaneous Pathology. 33:809_811.
6- Chen, D., Xi, T., Bai, J., (2007): Biological effects induced by nanosilver particles: in vivo study Biomedical Material. 2 :126–128
7- Chen, X., Schluesener, H.J., (2007): Nanosilver: A nanoproduct in medical application. Toxicology Letter. 176:1-12
8- Djeddi,A. N., Akrami Mogaddam, M. A., Ahmadi, F., Akradi, L., Ashrafi Helan, J.,(2008): Evaluation of the effectiveness of different levels of silver in (Nano scale) on weight gain and Bursa Fabricius development and on its histopathological lesions in broiler chicks, First International Veterinary Poultry Congress Tehran- Iran:p,127
9- El-Ansary, A., Al-Daihan, S., (2008): On the Toxicity of Therapeutically Used Nanoparticles:An Overview. Journal of Toxicology. 2009, 9 pages
10- Fisher,N.M., Marsh, E., Lazova, R., (2003): Scar-localized argyria secondary to silver sulfadiazine cream. Journal of American Academical Dermatology. 49:730-732
11- Hsin, Y.H., Chen, C.F., Huang, S., Shih, T.S., Lai, P.S., Chueh, P.J., (2008):The apoptotic effect of nanosilver is mediated by a ROS- and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells. Toxicology Letter. 10; 179(3):130-9.
12- Hussain, S.M., Hess, K.L., Gearhart, J.M., Geiss, K.T., Schlager, J.J., (2005): In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicology in Vitro 19:975-983,
13- Hussain, S.M., Javorina, M.K., Schrand, A.M., Duhart, H.M., Ali, S.F., Schlager, J.J., (2006): The interaction of manganese nanoparticles with PC-12 cells induces dopamine depletion. Toxicology Science 92:456-463
14- Ji, J.H., Jung, J.H., Kim, S.S., Yoon, J.U., Park, J.D., Choi, B.S., Chung, Y.H.,Kwon, I.H., Jeong, J., Han, B.S., Shin, J.H., Sung, J.H., Song, K.S., Yu, I.J.,(2007): Twenty-eight-day inhalation toxicity study of silver nanoparticles in Sprague-Dawley rats. Inhalation Toxicology 19:857_871
15- Kim, J.S., Kuk, E., Yu, K.N., Kim, J.H., Park, S.J., Lee, H.J., Kim, S.H., Park, YK, Park, Y.H, Hwang, C.Y., Kim, Y.K, Lee, Y.S., Jeong, D.H., Cho, M.H., (2007): Antimicrobial effects of silver nanoparticles.Nanomedicine 3:95_101
16- Kim, Y.S., Kim, J.S., Cho, H.S, Rha, D.S, Kim, J.M., Park, J.D., Choi, B.S.,Lim, R., Chang, H.K, Chung, Y.H, Kwon, I.H, Jeong, J., Han, B.S., YuI,J., (2008): Twenty-eight-day oral toxicity, genotoxicity, and gender-related issue distribution of silver nanoparticles in Sprague-Dawley rats. Inhalation Toxicology. 20:575_583.
17- Kim, W.Y., Kim, J., Park, J.D., Ryu, H.Y., Yu, I.J. (2009): Histological study of gender differences in accumulation of silver nanoparticles in kidneys of Fischer 344 rats. Toxicology Environmental Health;72(21-22)
18- Lam, P.K., Chan, E.S.Y., Ho,W.S., Liew, C.T., (2004):In vitro cytotoxicity testing of a nanocrystalline silver dressing (Acticoat) on cultured keratinocytes. British Journal of Biomedical Science 61(3):125_127.
19- Nagender R P.,Eladia M.P.-M., Josef H. (2008), silver or silver nanoparticles:a hazardous threat to the environment and human health? Journal of Applied Biomedicine:6(3):117-129
20- Park, S., Lee, Y.K., Jung, M., Kim, K.H, Eun-Kyung, Ahn, N.C., Lim, Y., Lee, K.H., (2007): Cellular toxicity of various inhalable metal nanoparticles on human alveolar epithelial cells. Inhalation Toxicology. 19(1):59_65.
21- Poon, V.K., Burd, A., (2004): In vitro cytotoxity of silver: Implication for clinical wound care. Burns 30:140_147.
22- Senjen, R., (2007): Nanosilver – a threat to soil, water and human health? Friends of the Earth Australia,
23- Shin,SH.,Ye,M.K.,Kim,H.S.,Kang,H.S., (2007):The effects of nano-silver on the proliferation and cytokine expression by peripheral blood mononuclear cells. International immunopharmacology.7(13):1813-1817
24- Soto, K., Garza, K.M., Murr, L.E., (2007): Cytotoxic effects of aggregated nanomaterials. Acta Biomater. 3:351_358
25- Sung, J.H., Ji, J.H., Yoon, J.U., Kim, D.S., Song, MY, Jeong, J., Han, BS., Han, J.H., Chung, Y.H., Kim, J., Kim, TS, Chang, HK., Lee, E.J., Lee, J.H., Yu, I.J., (2008): Lung function changes in Sprague-Dawley rats after prolonged inhalation exposure to silver nanoparticles. Inhalation Toxicology. 20:567_574
26- Susan, W.P., Wijnhoven, Willie, J.G.M., Peijnenburg,C. A., Herberts, Werner I. Hagens, Agnes G. Oomen,Evelyn H.W. Heugens, Boris R, Julia Bisschops, Ilse Gosens,Dik Van De Meent, Susan Dekkers, Wim H. De Jong,Maaike Van Zijverden, Adrie¨Nne J.A.M. Sips, & Robert E. Geertsma.,( 2009): Nanosilver a review of available data and knowledge gaps in human and environmental risk assessment Nanotoxicology.3(2): 109_138
27- Trop, M., Novak, M., Rodl, S., Hellbom, B., Kroell, W., Goessler, W., (2006): Silver coated dressing acticoat caused raised liver enzymes and argyria-like symptoms in burn patient. Journal of Trauma Injury Infect Critical Care 60:648_652
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