Identification of volatile compounds and investigation of antioxidant, antimicrobial and anticancer effects of Tribulus longipetalus Viv

Subject Areas : phytochemistry

Aliakbar Imani ¹

1 - Master of Chemistry, Kashan University, Kashan, Iran

Received: 2022-07-03 Accepted : 2022-09-15 Published : 2022-09-23

Keywords: Brine shrimp, cancer, Polystyrene divinylbenzene, Chromatography, Tribulus longipetalus Viv, Cellular toxicity, Ethyl acetate,

Abstract :

Objectives: The objective of the present study was to identify volatile compounds and investigate the antioxidant, antimicrobial and anticancer effects of Tribulus longipetalus Viv.Materials and methods: In this research, the Tribulus longipetalus Viv, which is an annual herbaceous plant, has many branches, lies on the ground, and has stems with soft hairs. The geographical spread of this plant is in the long sand dunes of Aran and Bidgol deserts. Biological properties, including free radical scavenging activity, total amount of phenolic compounds, antimicrobial properties and cytotoxic properties were investigated. Since the whole plant extract showed a high level of inhibition in the saltwater shrimp cell toxicity test (nearly 70 μg/liter), in order to obtain the effective components, solvent extraction using a decanter funnel was on the agenda. The three obtained components of hexane, ethyl acetate and water were analyzed separately. Also, by using a polystyrene divinylbenzene chromatography column, the effective component with higher inhibition ability was isolated. Among the effective components, except ethyl acetate, it had a relatively high inhibitory power, which was identified with the help of Hplc device.Findings: Comparing the antimicrobial power of Tribulus longipetalusViv with antibiotics, showed that this plant has good antimicrobial properties. Also, considering that LC50 was determined as 70 μg/ml. This value indicates the good cytotoxic power of Tribulus longipetalus Viv.

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