Investigation of Antioxidant Activity in Seed, Peel and Juice Extracts of Some Samples Iranian Pomegranate
Subject Areas : phytochemistry
1 - Qom Branch, Islamic azad University
Keywords: tannin, Saveh pomegranate, Yazd pomegranate, flavonoid, antioxidant activity, Qom pomegranate, Khorasan pomegranate, DPPH test,
Abstract :
Objective: In this research, a study was performed on several pomegranate populations from different cities of Iran, including: Qom, Saveh, Khorasan and Yazd (Qajagh poand black seed of Qom, Yazd Mikhosh and Khorasan glass pomegranate). For this purpose, characteristics such as the amount and type of compounds in the juice, antioxidant activity and evaluation of the active ingredients of tannins and flavonoids were considered.Material and Methods: In order to extract the juice, water and dried peel of the plant were drenched separately in water and methanol. The solvent was separated using a rotary and after drying the extract by vacuum oven, the samples were used in the antioxidant test of free radical reduction methods (DPPH).Results: The results of antioxidant test showed that sweet-and-sour Saveh pomegranate peel extract has the highest antioxidant effect in methanol solvent. Tannin test was performed on black seeds of Qom and Yazd. So, it was found that black seed pomegranate of Qom has the highest amount of tannin compounds. Based on the results of flavonoid test, black seed pomegranate had the highest amount. Finally, the chemical composition of black seed juice in Qom province was identified.Conclusion: These results showed that Qom black seed pomegranate has large amounts of natural sugars. Also, by comparing the amount of tannin in the samples, it was observed that the total amount in the sample of Yazd pomegranate is more than Qom black seed, but the amount of flavonoids in Qom black seed is more than Yazd pomegranate.
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