Effect of gibberellic acid and kinetin on some morphological features in two lentil cultivars under salinity stress
Subject Areas : biology
Fariba Shohani
1
(PhD. Student in Biology (Plant Physiology), Urmia University, Urmia, Iran.)
Keywords: germination, Lentils, Gibberellic acid, kinetine, salinity stress,
Abstract :
Objective: An experiment was conducted to study the effect of two plant growth regulators (gibberellic acid and quinine) on seed germination and initial seedling growth and salinity tolerance in both Kimia and Shirvan local cultivars.Materials and Methods: This experiment was performed as a factorial experiment with a completely randomized design. Two separate experiments were performed to investigate the response of different lentil genotypes to salinity stress applied with salt (Nacl) under different treatments of gibberellic acid (GA3) and kinetin growth regulators. In each of these experiments, 32 treatments were performed in three replications. The first experiment was performed as a factorial experiment in a completely randomized design with three factors: genotype (local and chemical), salinity (0,60, 120 and180 mmol) and gibberellic acid (0,2, 4 and 6 mg/l) The second experiment was performed in the same way with three factors: genotype (local and chemical), salinity (0,60, 120 and 180 mmol) and kinetin (0,2, 4 and 6 mg/l)Findings: The results of the study of the main and interaction effects of genotype, salinity and growth regulator of gibberellic acid on the evaluated traits of lentil showed that the alchemy genotype had the highest mean in the study of traits (dry weight, root to shoot, root length). What is the total seedling length and germination percentage and the best genotype in terms of maximum mean in the study of traits (relative water content, tissue water content, stem length, number of roots and germination percentage) It was related to Ilam genotype.Conclusion: Statistical analysis of the studied traits at germination and seedling growth stage showed that salinity reduced the rate of germination and seedling growth in both cultivars. The results showed that the inhibitory effects of salt on seedling growth of Shirvan local cultivar were significantly higher than Kimia cultivar, which in fact indicates the similarity of salinity tolerance at germination and full plant stages in these two cultivars.Gibberellic acid increased germination rate in both cultivars, but Kimia genotype had the highest mean in the study of traits (root length, total seedling length, root-to-stem ratio). However, Shirvan local genotype had the highest mean in the study of traits such as (relative water content, tissue water content, stem length, number of roots). The trend of reduced root and stem growth in comparison with the control due to gibberellic acid treatment showed that the local cultivar was more sensitive to this growth regulator. Significant interaction was observed between different levels of salinity and gibberellic acid, meaning that the growth response of seedlings was different under the combined effects of salinity and gibberellicacid.
Abrahimzade H. Plant physiology. Tehran:Tehran university Press, 1991. [In Persian].
Asadian NW & Miyamoto S. Salt effects on alfalfa seedling emergenc. Agronomy Journal. 1987; 76: 710-714.
Baharvandi M. Investigation of the effect of supplementary irrigation and phosphorus, chemical and biological fertilizers on the quantitative and qualitative characteristics of lentil cultivars in rain-fed conditions as autumn crops. Master thesis. Agriculture field, 2014. [In Persian]
kafi C & Salehi M. Agricultural Agriculture. Mashhad: Mashhad University Press, 2000. [In Persian]
Koochaki A & Banayan M. Beans cuitivation. Mashhad: Mashhad university Jihad press, 2009. [In Persian]
Lacher, W. Physiological plant ecology. Springer Publishing, 1995: 390.
Lessani H & Mojtahedi M. Green plant life. (Galston-Davis-Sotter). Tehran: Tehran University, 2011: 578. [In Persian]
Mehrabi, AA, Yazdi-Samadi. B, Naghavi MR, Omidi M & Tavakol-Afshari R. Abscisic Acid and Kinetin Effects on Seed Germination and Seedling Early Growth of Wheat under Salinity Stress. Pajouhesh & Sazandegi. 2007; 77: 83-93. [In Persian]
Parsa M & Bagheri AR. Bean. Mashhad: Mashhad University, 2008: 528. [In Persian].
Raou IM. Effect of IAA on wheat yield in saline soils. Plant Physiology. 1961; 62: 814-819.
Rashidi V. Germination reaction and seedling traits of native lentil genotypes to drought and salinity stress and causal analysis of seedling weight in vitro conditious. Journal of crop breeding. 2018; 10. [In Persian].
Shiroei, H. Evaluation of the effect of supplementary irrigation and plant density on growth characteristics and yield of lentil cultivars in Ilam climate. Master Thesis. Field of Agriculture, Ilam Faculty of Agriculture, 2012. [In Persian].
Tahmas Vand M. The effect of salinity overgrowth on growth and germination improvement of Medik Lens Culinaria lentils under salinity stress conditions, 2013. [In Persian].
Tuna, AK. The combined effects of gibberellic acid and salinity on some antioxidant enzyme activities, plant growth parameters and nutritional status in maize plant. Environmental and Experimental Botany. 2019; 62(1): 1-9
Zakeri, M & Parsons LR. Comparative Effects of NaCI and Polyethylene Glycol on Root Distribution, Growth and Stomata conductance of Sour Orange Seedlings. Plant and Soil. 1990; 129: 137-143.
_||_Abrahimzade H. Plant physiology. Tehran:Tehran university Press, 1991. [In Persian].
Asadian NW & Miyamoto S. Salt effects on alfalfa seedling emergenc. Agronomy Journal. 1987; 76: 710-714.
Baharvandi M. Investigation of the effect of supplementary irrigation and phosphorus, chemical and biological fertilizers on the quantitative and qualitative characteristics of lentil cultivars in rain-fed conditions as autumn crops. Master thesis. Agriculture field, 2014. [In Persian]
kafi C & Salehi M. Agricultural Agriculture. Mashhad: Mashhad University Press, 2000. [In Persian]
Koochaki A & Banayan M. Beans cuitivation. Mashhad: Mashhad university Jihad press, 2009. [In Persian]
Lacher, W. Physiological plant ecology. Springer Publishing, 1995: 390.
Lessani H & Mojtahedi M. Green plant life. (Galston-Davis-Sotter). Tehran: Tehran University, 2011: 578. [In Persian]
Mehrabi, AA, Yazdi-Samadi. B, Naghavi MR, Omidi M & Tavakol-Afshari R. Abscisic Acid and Kinetin Effects on Seed Germination and Seedling Early Growth of Wheat under Salinity Stress. Pajouhesh & Sazandegi. 2007; 77: 83-93. [In Persian]
Parsa M & Bagheri AR. Bean. Mashhad: Mashhad University, 2008: 528. [In Persian].
Raou IM. Effect of IAA on wheat yield in saline soils. Plant Physiology. 1961; 62: 814-819.
Rashidi V. Germination reaction and seedling traits of native lentil genotypes to drought and salinity stress and causal analysis of seedling weight in vitro conditious. Journal of crop breeding. 2018; 10. [In Persian].
Shiroei, H. Evaluation of the effect of supplementary irrigation and plant density on growth characteristics and yield of lentil cultivars in Ilam climate. Master Thesis. Field of Agriculture, Ilam Faculty of Agriculture, 2012. [In Persian].
Tahmas Vand M. The effect of salinity overgrowth on growth and germination improvement of Medik Lens Culinaria lentils under salinity stress conditions, 2013. [In Persian].
Tuna, AK. The combined effects of gibberellic acid and salinity on some antioxidant enzyme activities, plant growth parameters and nutritional status in maize plant. Environmental and Experimental Botany. 2019; 62(1): 1-9
Zakeri, M & Parsons LR. Comparative Effects of NaCI and Polyethylene Glycol on Root Distribution, Growth and Stomata conductance of Sour Orange Seedlings. Plant and Soil. 1990; 129: 137-143.