Comparative anatomical study of leaf and petiole in three species of pear (Pyrus L.) in Guilan Province

Subject Areas : botany

Nasibeh Dehghan ¹ , Siavash Hosseini Sarghein ² , Rashid Jamei ³

1 - Masters of Plant Physiology, Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran
2 - Associate Professor, Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran
3 - Associate Professor, Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran.

Received: 2020-09-29 Accepted : 2020-12-19 Published : 2020-09-22

Keywords: Guilan Province, Pyrus, leaf, Petiole,

Abstract :

Objectives: The aim of this study was comparing the anatomical characters of leaves and petioles of 3 species of pear (Pyrus boissieriana Buhse, Pyrus communis L, Pyrus hyrcana Fedor) in Guilan Province. Materials and Methods: In this study, for anatomical study of species, at first, plant samples were collected. Then the manual section was performed in the laboratory. Carmine staining was used in order to stain the samples. After staining, slides were prepared from the cross-sectional area. For observing the transverse sections of leaves and petiole, optical microscope equipped with a scaled lens was used. Then, the data obtained from anatomical characters were statistically analyzed. Results: Leaf and petiole thickness were different among three tested species. The highest midrib thickness belonged to p < /em>. communis (682±1.11μm). The lowest midrib thickness was observed in p < /em>. hyrcana (431.66±1.05μm). Anatomical characters are effective and important in species segregation. Therefore, anatomical characteristics in different plant species will be useful in terms of taxonomic classification.    

References:

1. Aydin ZU & Donmez AA. Taxonomic and biogeographic notes on the genus Pyrus L. (Rosaceae): A new record and a new synonym, with data on seed morphology. Plant & Fungal Research. 2019; 2(1): 2-8.


2. Fahn A & Arlee T. Vascularization of articulated Chenopodiaceae and nature of their fleshy cortex. American Journal of Botany. 1950; 46: 330-338.


3. Hosseini Sarghein S, Carapetian J & Khara J.The effects of UV radiation on some structural and ultrastructural parameters in Pepper (Capsium longum A. DC). Turkish Journal of Biology. 2011; 35: 69-77.


4. Jafari A. Plant Anatomy. Third Edition. Mashhad: University Jihad Publications; 2008.
   [In Persian]


5. Kasalkheh R, Jorjani E, Sabouri H, Habibi M & Sattarian A. Anatomical study of Rubus subgenus Rubus in Iran. Taxonomy and Biosystematics. 2016; 8(27): 19-38. [In Persian]


6. Khatamsaz M. Flora of Iran no. 6: Rosaceae. Tehran: The Research Institute of Forests and Rangelands Press; 1992. [In Persian]


7. Mozaffarian V. Trees and shurbs of Iran. Tehran: Farhang moaser Press;2006.[In Persian]


8. Murti RH, Kim HY & Yeoung YR. Morphological and anatomical characters of ploidy mutants of strawberry. International Journal of Agriculture and Biology. 2012; 14(2): 204-210.


9. Raei Niaki N, Attar F & Maroofi H. Anatomical studies on fourteen species of the genus cotoneaster L. (Rosaceae). Iranian Journal of Botany.2009; 15(1): 96-104.


10. Rahimi Nejad M. Plant Systematics. Isfahan: Isfahan University Press; 2005: 61-71.
    [In Persian].


11. Saeedi H. Plant Systematics. Isfahan: Jahad Daneshgahi, Industrial Branch; 2003: 311-345. [In Persian]


12. Vafadar M, Attar F, Maroofi H & Aghabeigi F. Leaf anatomical study of the genus Amygdalus L. (Rosaceae) in Iran and its taxonomical implication. Iranian Journal of Botany. 2008;14(2): 143-155.


13. Zamani A, Attar F, Ghahreman A & Maroofi H. Anatomical studies of the Genus Pyrus L. (Rosaceae) in Iran its taxonomical implications. Iranian Journal of Botany. 2008; 14(2): 132-142.


14. Zargari A. Medicinal plants. (ed.³) Tehran: Tehran University Publications; 1996.