Study of antioxidan and antimicrobial effects of aqueous and alcoholic extract of plant ( Lawsonia inermis, punica granatum, walnut, Myrtus) on gram positive and gram negative bacteria

Subject Areas : microbiology

simin khosravi ¹ , zahra rezayatmand ² , mozhgan ghiasian ³

1 - Department of Microbiology,Falavarjan beranch, Islamic Azad University,Isfahan, Iran
2 - Department of Biology,Falavarjan branch,Islamic Azad University,Isfahan, Iran
3 - Department of Microbiology,Falavarjan beranch, Islamic Azad University,Isfahan, Iran

Received: 2019-07-07 Accepted : 2020-04-26 Published : 2020-02-20

Keywords: Antimicrobial agents, Pathogenic bacteria, water extract, Alcoholic extract,

Abstract :

Recently , due to the resistance of bacterial pathogens to the detection of new antimicrobials, much attention has been paid. In the present study, the antimicrobial properties of four extracts of leafy leafy leaves, henna leaves, walnut shells and pomegranate peel on three gram-positive bacteria, Staphylococcus aureus, Staphylococcus epidermidis and Bacillus subtilis, and two gram-negative bacteria of Escherichia coli and Pseudomonas aeruginosa were studied. it placed. Antimicrobial effect of the alcoholic and aqueous extract of these plants was studied by diffusion and well discarding method by measuring the non-growth aura's diameter and the minimum inhibitory concentration was investigated by ELISA method. The results show that in two methods, diffusion discs and wells in alcoholic and aqueous extracts have the highest effect on Staphylococcus epidermidis bacterium and have the least effect on E. coli bacteria. The minimum amount of MIC for alcoholic extract is 31.25 and MBC 54.25 mg / ml, which is related to Escherichia coli bacteria. The results measurements of total phenolic compounds, flavonoids and tannins also indicate that the highest amount of this product is in the concentration of 500 mg / ml of the extract of plants. Also, the amount of antioxidant activity in the alcoholic extract is about twice as much as the aqueous extract. The results of this study indicate that the combination of extracts of plants, especially alcoholic extract, has a more antibacterial effect than the aqueous extract, which can be due to the presence of more antibacterial compounds in the extract.