Molecular Identification of the TEM and CTX Genes in Klebsiella pneumoniae Strains Isolated From Clinical Samples of Hospitals in Iranshahr City
Subject Areas : microbiology
Mahnaz
Thaghimosleh
1
(Microbiology Dept., Sirjan Branch, Islamic Azad University, Sirjan. Iran)
laleh
khajehkarimaldini
2
(Assistant Professor, Department of Microbiology, Sirjan branch, Islamic Azad University, Sirjan, Iran)
Keywords: TEM, CTX, Antibiotic resistance, Klebsiella pneumoniae, PCR,
Abstract :
Background: Klebsiella pneumoniae is a gram-negative pathogen and common cause of nosocomial infections. Increasing the emergence of multi-drug resistance in Klebsiella pneumonia has limited therapeutic options for this bacterium. The aim of this study was to molecular identification of TEM and CTX genes in Klebsiella pneumoniae isolated from clinical samples of hospitals in Iranshahr city. Materials and Methods: 50 strains of Klebsiella pneumoniae within 6 months, were collected from patients admitted to the intensive care unit of hospitals in Iranshahr. The isolates were identified as Klebsiella pneumoniae based on biochemical tests. Antibiotic susceptibility test was performed by disc diffusion method. To molecular identification of TEM and CTX genes, PCR was performed. Results: Based on the results of PCR, 43 (86%) of the samples had phenotypic ESBL enzymes, 20 (47%) had CTX gene, 8 (18%) had TEM gene, and 15 (34%) had Both the CTX and TEM genes. Conclusion: The results of this study showed a high prevalence (P <0.05) of multi-drug resistant Klebsiella pneumoniae and ESBL-resistant isolates in ICU patients, which emphasizes the appropriate policies for controlling infection.
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