Biosynthesis of Silver Nanoparticles by a native Halotolerant Actinomycete strain Isolated from Soils Qom, Iran

Subject Areas : Nanobiotchnology

sheida nadernia ¹ , soheil Aghaei ² , Mohammad Ali Gasemzadeh ³

1 - master of Microbiology /Islamic Azad University of Qom (IAU Qom) /Basic Sciences/ Qom / Iran
2 - Faculty member
3 - 3Department of Applied Chemistry, Faculty of Chemistry, Islamic Azad University, Qom, Iran.

Received: 2018-12-18 Accepted : 2019-03-01 Published : 2019-06-20

Keywords: halotolerant actinomycetes, biosynthesis, Keywords: silver nanoparticles,

Abstract :

Abstract Background:In recent years, ability of microorganisms to synthesize nanoparticles of various sizes, shapes and morphologies, has gained extreme attentions. the point of this study is Biosynthesis of Silver Nanoparticles by a native Halotolerant Actinomycete strain Isolated from Soils of Saline Lake Qom Materials & methods: In the present study, synthesis and characterization, of silver nanoparticles from native isolate of halotolerant Actinomycete strain has been reported. Silver nanoparticles were synthesized by adding the dried biomass of halotolerant actinomycete isolate with 10-3M of nitrate solution adjusted pH 7.2 and in M9 liquid medium. Silver nanoparticles were characterized using ultraviolet and visible absorption spectroscopy, infrared spectroscopy (UV-VIS) Fourier Transform Infrared Spectroscopy(FT-IR), beam X Ray Diffraction(XRD), Dynamic light scattering (DLS), and imaging with Transmission Electron Microscope (TEM). Results:The results obtained from ultraviolet and visible absorption spectroscopy, infrared spectroscopy (UV-VIS) Fourier Transform Infrared Spectroscopy(FT-IR) and beam X Ray Diffraction (XRD), Of the halotolerant actinomycetes strains, the isolate NO.3 was able produce silver nanoparticles under different environmental conditions. Dynamic light scattering (DLS), and imaging with Transmission Electron Microscope (TEM) showed that the average size of silver nanoparticles obtained 28 to100 nm. The results indicated that production of silver nanoparticles was extracellular. Conclusion:The results showed that the native halotolerant actinomycete strains can synthesize silver nanoparticles under different environmental conditions . Keywords: silver nanoparticles, Biosynthesis, halotolerant actinomycetes.