study the effect of silver nanoparticles against to biofilm in listeria monocytogenes

Subject Areas : microbiology

razieh  ahmadinasab ¹ (microbiologi, university of qom, iran)
soheil  Aghaei ² (گروه میکروب شناسی، دانشکده علوم پایه، واحد قم، دانشگاه آزاد اسلامی، قم، ایران)
Mohammad Ali  Gasemzadeh ³ (3Department of Applied Chemistry, Faculty of Chemistry, Islamic Azad University, Qom, Iran.)

Received: 2018-10-02 Accepted : 2018-11-30 Published : 2019-02-20

Keywords: Antimicrobial Activity, Listeria monocytogenes, Antibiofilm, silver nanoparticles,

Abstract :

Listeria monocytogenes has been isolated from many foods and may cause meningitis, septicemia and abortion in pregnant women. L. monocytogenes forms biofilm on many food contact surface materials and medical devices. Biofilm formation by this bacterium on many surfaces, is one of the most important problems in the food industry. Metal nanoparticles are effective antimicrobial compounds for controlling and removing of bacterial biofilms from non-biomaterial surfaces. The main purpose of this investigation was synthesis of silver iodide nanoparticles in-vitro condition and evaluation of their antimicrobial properties for inhibition of biofilm formation and biofilm eradication of Listeria monocytogenes(ATCC1298). Method: In this study nano-particles of silver iodide were synthesized respectively sonochemically and were confirmed using UV-Vis ,FTIR, EDX and Scanning Electron Microscopic procedures. Antimicrobial and antibiofilm properties of synthesized nanoparticles were determined using agar well diffusion and 96 well microplate dilution methods respectively. Results: Synthesized Silver nanoparticles had circular structure with 30 nm size. Antibiofilm activity and biofilm eradication of Iodide silver nanoparticles were obtained at 5 and 100 µg/ml respectively. Discussion: Based on obtained results, silver iodide nanoparticles can be used as the effective antimicrobial agents for inhibiting of biofilm formation of Listeria monocytogenes in the food industry.