Synthesis of zinc oxide nanoparticles with antimicrobial and antibiofilm activity against Klebsiella pneumonia
Subject Areas : microbiologyrazieh ahmadinasab 1 , soheil Aghaei 2 , Mohammad Ali Gasemzadeh 3
1 - microbiologi, university of qom, iran
2 - Faculty member
3 - 3Department of Applied Chemistry, Faculty of Chemistry, Islamic Azad University, Qom, Iran.
Keywords: Antimicrobial Activity, Klebsiella peneumoniae, Zinc Oxide Nanoparticles, Antibiofilm,
Abstract :
Klebsiella peneumoniae bacterium is part of the natural microfrola of the human body and are found in water, sewage, soil and part of the mouth, pharynx and intestines. Klebsiella can also cause infections in the urinary tract, lower biliary tract, and surgical wound sites. It is also an opportunistic pathogen for patients with chronic pulmonary disease, enteric pathogenicity, nasal mucosa atrophy, and rhinoscleroma. This organism can causes food poisoning and gastritis. Biofilm formation by this bacterium on many surfaces, is one of the most important problems in the food industry. Metal nanoparticles are effective antimicrobial compounds for controlling and removing of bacterial biofilms from non-biomaterial surfaces. The main purpose of this investigation was synthesis of zinc oxide nanoparticles in-vitro condition and evaluation of their antimicrobial properties for inhibiting of biofilm formation and biofilm eradication of Klebsiella peneumoniae (ATCC700603). Method: In this study nano-particles of zinc oxide were synthesized mechanochemically and were confirmed using UV-vis, FTIR, EDX and Scanning Electron Microscopic procedures. Antimicrobial and antibiofilm properties of synthesized nanoparticles were determined using agar well diffusion and 96 well microplate dilution methods respectively. Results: Synthesized zinc oxide nanoparticles had circular structure with 30 nm size. Biofilm formation of kiebsiella pneumonia was performed using micrtiter plate assay. Antibiofilm activity and biofilm eradication of zinc oxide nanoparticles were obtained at 50 and 500 µg/ml respectively. Conclusions: Our study showed that zinc oxide nanoparticles can be used as the effective antimicrobial agents for inhibiting of biofilm formation of Klebsiella peneumoniae in the food industry.
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