Molecular identification of adhesion, necrosis and hemolysin genes in UPEC and APEC strains isolated from clinical specimens and poultry

Subject Areas : microbiology

kumarss amini ¹ , Gholamaliye Moradli ² , Sepideh Shamsgoli ³

1 - Assistant Professor, Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran.
2 - Assistant Professor, Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran.
3 - Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran

Received: 2017-04-07 Accepted : 2017-05-26 Published : 2017-08-23

Keywords: Escherichia coli (UPEC), Multiplex-PCR, Virulence genes,

Abstract :

Background and Aim: Uropathogenic Escherichia coli (UPEC) and Avian pathogenic Escherichia coli (APEC) is produce a wide range of human and animal virulence factors. These factors create colonization, invasion and the subsequent decline in the host immune response. These factors are include pap, sfa, afa, hly and cnf genes. The aim of this study was to molecular identification of adhesion, necrosis and hemolysin genes in UPEC an APEC isolated from poultry and humans samples. Materials and Methods: A total of 36 isolates from clinical samples from patients with urinary tract infections in hospitals in Kerman and 36 poultry isolates were obtained from the veterinary faculty of Kerman province. Isolates were identified by standard biochemical tests. In order to identify, in vitro amlification Multiplex-PCR were performed for pap, sfa, afa, hly and cnf genes. Results: Genes analysis showed that in the poultry samples, 10 (27.7%) of the isolates contained acute genes and 22 (61.1%) of the isolates were in the human specimens in terms of presence of the gene. Cfa gene and cnf were not detected poultry and humans in any of the samples. The highest frequency of pap gene was reported in 33.33% in poultry samples and 13.8% in human samples. Conclusion: The PCR method is a way in early detection of virulence factors in Escherichia coli isolates from animal and human but Epidemiological studies and rapid struggle with the disease can be used to determine the distribution and source of contamination.