ESBL prevalence and molecular characterization of beta-lactamase gene blaTEM in urinary isolates of Klebsiella pneumoniae in Qom

Subject Areas : biology

منصوره نرگسیان ¹ , Mohsen Zargar ² , Mahmoud Saffari ³

1 - دانشجوی کارشناسی ارشد
2 - عضو هیات علمی- دانشگاه آزاد اسلامی واحد قم
3 - عضو هیات علمی داشگاه علوم پزشکس دانشگاه کاشان

Received: 2015-10-02 Accepted : 2015-12-01 Published : 2014-04-21

Keywords: Antibiotic resistance, Urinary Tract Infection, Klebsiella pneumoniae, ESBL,

Abstract :

Klebsiella is opportunistic pathogen and one of the most common causes of nosocomial infections this pathogen cause a variety of diseases including urinary tract infections.Today, the prevalence of ESBL-producing pathogens (ESBLS) is important, so these infections are associated with prevalence of diseases and increase healthcare costs associated. The aim of this study was to determine the prevalence of genes blaTEM isolates of Klebsiella pneumoniae isolated from urinary tract infections in Qom.After identification of isolates using culture and biochemical methods, 140 isolates of Klebsiella pneumoniae was identified. The sensitivity of the isolates to different antibiotics was performed by disk diffusion method based on the standard (CLSI2013). The phenotypic confirmatory test ESBL strains have done followed by PCR method to detection of blaTEM gene. Out of 300 samples, 140 isolate identified as Klebsiella pneumoniae that 52 (%37.14) isolates were ESBL. The most antibiotic resistance was related to ceftazidime (%50.72). Out of 52 ESBL positive strains, 32 (61.53%) strains were shown to have blaTEM gene.Considering the high prevalence of ESBL-producing isolates in hospitals, early detection and follow-up to prevent the spread of resistant isolates it all the more essential. It must also change in the pattern of antibiotics, hospital infection control measures are highly recommended.