Evaluation of antimicrobial and presence of phytochemical compounds of collected lichens from Haft Chemshme in Tabriz Province and their effect on some enteric pathogenic bacteria
Subject Areas : biology
Keywords: antimicrobial effect, extracts, Tabriz native lichen,
Abstract :
Introduction: Lichens are a set of algae and fungi, form long times lichens are used in traditional medicine, therefore, identifying the lichens and their ability to produce antimicrobial product is important.Methods and Materials: In the present study effect of four lichen types, which has been collected from around Tabriz and identified by Botanical Center of Iran, Tehran were evaluated. Then effect of watery, alcholoic and acetonic extract of each lichen were evaluated on 3 pathogenic bacteria belong to: Escherichia coli ( PTCC 1395), Shigella dysenetriae (PTCC 1188) and Salmonella typhi ( PTCC 1609) using well diffusion agar method and their MIC ( 0.5- 0.0156 g/ml) were determined. In addition, the presence of phytochemical compound such as: flavonoid, terpenoid and tannin were evaluatedResults: The results obtained from this study indicated that out of all extracts, watery extract had no effect on bacteria but ethanolic and specially acetonic had more effect on bacteria such as: E. coli and S. typhi. On the other hand, results showed that lowest MIC belong to acetonic extract of Staurothele on E.colli . Additionally, presence of flavonoid and trepenoid confirmed in all of lichens and presence of tannin was confirmed only by Staurothele..