Isolation and Identification of Pathogenic and non Pathogenic Vibrio from Persian gulf and the evalutation of their chemotactic behavior
Subject Areas : biology
Keywords: vibrio mimicus, vibrio species, Vibrio alginolyticus, BM device, chemotaxis behavior,
Abstract :
Genus Vibrio is a gram negative, curved, motile and non spore former bacteria. Natural habitat of these bacteria is environments such as sea and rivers .However, some of Vibrios are pathogen ,but most of them are non pathogen for human being. However recently chemotaxis behavior of these bacteria has been considered as invasive factor.This behavior helps the bacteria to move toward of suitable conditions to increase their survive in the environment .The present study conducted to evaluate the chemotaxis behavior of Vibrio spp. isolated from Persian gulf to perform the study 50 samples were collected and 2 Vibrio spp. were isolated and phenotypic and genoty identified. Chemotaxis behavior of the isolates was evaluated using diffusion agar method and (BM) device. the chemotaxis behavior of the isolate was evaluated against Phenylalanine, Cysteine , lysine ,Glycine, Galactose, Maltose, Rhamnose and Trehalose .The result optained indicated that 2 species of Vibrio alginolyticus and Vibrio mimicus were isolated. Vibrio alginolyticus exhibited positive chemotaxis behavior against lysine ,Glycine, Maltose, Trehalose, Galactose and negative chemotaxis behavior to Phenylalanine, Cysteine and Rhamnose. Vibrio mimicus showed positive chemotaxis against Phenylalanine, Cysteine ,Maltose, Rhamnose and Trehalose and negative chemotaxis behavior to lysine ,Glycine and Galactose.