Bacillus phytase production optimization in Pantatnykvs

Subject Areas : biology

Received: 2013-09-30 Accepted : 2013-11-29 Published : 2013-12-22

Keywords: phytase, Optimization, Bacillus,

Abstract :

Phytase on a particular set of alkaline hydrolysis step can be done phosphate from phytate molecule of the day, the main source of phytate phosphorus in grain storage plants. This enzyme categories among plants, microorganisms and animals extent of their distribution. The aim of this study is to optimize the production of phytase in Bacillus Pantatnykvs. The optimum conditions of temperature, pH, the shakers, the source of carbon, nitrogen, phosphorus, different salt concentrations, different concentrations of heavy metals was determined that Bacillus Pantatnykvs Nacl at 37 ° C, 5/7 = pH, Shaker away rpm150, glucose carbon source, nitrogen source ammonium nitrate, calcium phytate phosphorus, sodium chloride, 10% = Nacl in the presence of metal cadmium chloride had the highest enzyme production. Optimize enzyme activity in terms of temperature, pH, substrate concentration, the salt concentration was carried out at 80 ° C, pH = 9, 1050 microliter of substrate concentration and concentration of 0% sodium chloride highest enzyme activity was observed.