Molecular screening and identification of indigenous bacteria producing carotenoid pigments and comparing their biological effects with pigments extracted from plants (pomegranate, beetroot, and barberry)
Subject Areas : biology
Neda Chitsaz
1
,
مهرداد معماریان
2
*
,
علی جوادی
3
1 - Department of Microbiology, Faculty of Basic Sciences, Qo.C., Islamic Azad University, Qom, Iran.
2 - Department of Microbiology, Qo.C., Islamic Azad University, Qom, Iran
3 - Department of Microbiology, Qo.C., Islamic Azad University, Qom, Iran.
Keywords: Pigment, Bacteria, Actinomycetes, Isolation, Molecular Identification,
Abstract :
The use of natural pigments as alternatives to chemical drugs in controlling bacterial growth has attracted much attention. This study aimed to identify indigenous bacteria producing carotenoid pigments and compare the effects of these pigments with those extracted from pomegranate, beet, and barberry plants. Fifty soil samples were collected from different areas of Qom farms. Actinomycetes isolates were selected using SCA-specific culture medium and biochemical methods. Pigment-producing isolates were identified using ISP2 culture medium, and methanol extracts of their plant and bacterial pigments were extracted. The type of bacterial pigment was determined using Fourier Transform Infrared spectroscopy (FT-IR). The antibacterial and antioxidant effects of bacterial and plant pigments were evaluated. The best isolate was molecularly identified using the 16s rRNA gene identification. The obtained data revealed that the extracted bacterial and plant pigments have significant antioxidant effects, and the bacterial pigment also showed a growth inhibitory effect against pathogens Staphylococcus aureus and Escherichia coli, with a halo diameter of more than 30 mm Sequencing of the selected bacteria showed that the obtained isolate has 100% similarity to Streptomyces gramineus. This study shows that the carotenoid pigments produced by the native bacteria and evaluated plants have strong antioxidant effects and can be used in therapeutic processes or as food additives. It is suggested that further studies be conducted to identify the active compounds of these pigments and investigate the precise mechanisms of their action.
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